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Identification and Cloning of jipA Encoding a Polypeptide That Interacts with a Homolog of Yeast Rad6, UVSJ in Aspergillus nidulans  

Cho, Jae-Han (Research Center for Biomedicinal Resources)
Yun, Seok-Soong (Research Center for Biomedicinal Resources)
Jang, Young-Kug (Korea Research Institute of Bioscience and Biotechnology)
Cha, Mee-Jeong (Research Center for Biomedicinal Resources)
Kwon, Nak-Jung (Research Center for Biomedicinal Resources)
Chae, Suhn-Kee (Division of Life Sciences, Paichai University)
Publication Information
Journal of Microbiology / v.41, no.1, 2003 , pp. 46-51 More about this Journal
Abstract
RAD6 in yeast mediates postreplication DNA repair and is responsible for DNA-damage induced mutations. RAD6 encodes ubiquitin-conjugating enzyme that is well conserved among eukaryotic organisms. However, the molecular targets and consequences of their ubiquitination by Rad6 have remained elusive. In Aspergillus nidulans, a RAD6 homolog has been isolated and shown to be an allele of uvs). We screened a CDNA library to isolate UVSJ-interacting proteins by the yeast two-hybrid system. JIPA was identified as an interactor of UVSJ. Their interaction was confirmed in vitro by a GST-pull down assay. JIPA was also able to interact with mutant UVSJ proteins, UVSJl and the active site cysteine mutant UVSJ-C88A. The N- and the C-terminal regions of UVSJ required for the interaction with UVSH, a RAD18 homolog of yeast which physically interacts with Rad6, were not necessary for the JIPA and UVSJ interactions. About 1.4 kb jipA transcript was detected in Northern analysis and its amount was not significantly increased in response to DNA-damaging agents. A genomic DNA clone of the jipA gene was isolated from a chromosome I specific genomic library by PCR-sib selection. Sequence determination of genomic and cDNA of jipA revealed an ORF of 893 bp interrupted by 2 introns, encoding a putative polypeptide of 262 amino acids. JIPA has 33% amino acid sequence identity to TIP41 of Saccharomyces cerevisiae which negatively regulates the TOR signaling pathway.
Keywords
Aspergillus nidulans; RAD6; TIP41; uvsJ; DNA repair; TOR pathway;
Citations & Related Records

Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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