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Effect of pH on production of gellan by Pseudomnas eldoea ATCC 31461

  • Im, Seong-Mi;Lee, Ji-Hyeon;Kim, Seong-Gu
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.201-204
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    • 2002
  • The gellan was extracellular polysaccharide produced by Pseudomonas elodea A TCC 31461 at aerobic condition. Gellan provides various functionalities such as gelling, suspending, stabilizing, emulsifying and binding properties in aqueous systems. In this study, the effect of pH on the cell growth and the gellan production were evaluated in shake- flasks and in 5 ${\ell}$ batch fermentor. In the shake-flasks culture, maximum gellan production was obtained with 1.66g/ ${\ell}$ when initial pH was 7.0. The batch fermentation was performed in the medium pH control ranged pH 5.5-8.5. The maximum gellan production of 1.97g/l was obtained with constant pH 6.0.

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Effects of Basal Media, Auxin, Sucrose and Agar Concentrations and pH Value on Asymbiotic Germination and Subsequent Growth of Dendrobium kingiurmm (Dendrobium kingianum의 발아(發芽)와 유묘생장(幼苗生長)에 미치는 기본배지(基本培地) 및 auxin, sucrose 한천농도(寒天濃度)와 pH의 영향(影響))

  • Chung, Jae Dong;Chun, Chae Ki;Suh, Young Kyo;Choi, Soo Ok
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.1-7
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    • 1985
  • The study was conducted in order to get basic information on seed germination and seedling growth through the experiments such as basal medium, concns of auxin, sucrose agar and pH values. Seed germination was very excellent in $3g/{\ell}$ hyponex and $5g/{\ell}$ peptone containing medium. Multiple shoots were formed in $1-3g/{\ell}$ hyponex and $5g/{\ell}$ pepton containg medium supplemented with $1.0g/{\ell}$ IBA, $30g/{\ell}$ sucrose and $8g/{\ell}$ agar. Growth of seedlings was more stimulated in the same amount of hyponex and peptone as above medium substituted with $40g/{\ell}$ sucrose and $6g/{\ell}$ agar without IBA.

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형질전환 식물세포배양을 이용한 Human Granulocyte Colony Stimulating Factor의 생산

  • Hong, Sin-Yeong;Gwon, Tae-Ho;Jang, Yong-Seok;Yang, Mun-Sik
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.447-448
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    • 2001
  • Human garnulocyte-colony stimulating factor(hG-CSF), a hematopoietic growth factor$^{1)}$, was produced and secreted from tobacco cell suspension. hG-CSF produced from tobacco cell suspension culture is biologically active form. The produced amount of hG-CSF is about 100${\mu}g/L$ in 9 days after inoculation.

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HARMONIC TRANSFORMATIONS OF THE HYPERBOLIC PLANE

  • Park, Joon-Sik
    • Journal of the Chungcheong Mathematical Society
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    • v.22 no.4
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    • pp.771-776
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    • 2009
  • Let (H, g) denote the upper half plane in $R^2$ with the Riemannian metric g := ($(dx)^2$ + $(dy)^2$)$/y^2$. First of all we get a necessary and sufficient condition for a diffeomorphism $\phi$ of (H, g) to be a harmonic map. And, we obtain the fact that if a diffeomorphism $\phi$ of (H, g) is a harmonic function, then the following facts are equivalent: (1) $\phi$ is a harmonic map; (2) $\phi$ is an affine transformation; (3) $\phi$ is an isometry (motion).

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The Technical Trends for Intercell Interference Control including Small Cells (소형셀 간섭제어 기술동향)

  • Jwa, H.K.;Kim, S.H.;Na, J.H.
    • Electronics and Telecommunications Trends
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    • v.31 no.5
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    • pp.51-58
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    • 2016
  • 모바일 기기와 데이터 이용량의 폭발적인 증가에 따라 제한된 자원으로 좀 더 빠르고 많은 용량을 처리하기 위한 다양한 기술 중 단위 면적당 용량 증대가 가능한 소형 셀 기술이 3G/4G 이동통신 시스템뿐만 아니라 5G 이동통신에서도 더욱 밀집한 셀 구성과 셀 소형화 형태로 연구 중에 있다. 이에 따라 셀 간 간섭을 제어하는 기술에 대한 연구도 꾸준히 진행되고 있다. 본고에서는 셀 간 간섭제어 기술에 대한 표준화 동향과 5G 이동통신에서의 간섭제어 기술에 대한 동향 및 방향에 대해 소개하고자 한다.

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Soil Microbial Community Analysis using Soil Enzyme Activities in Red Pepper Field Treated Microbial Agents (토양효소활성을 이용한 미생물제제 처리 고추경작지의 토양미생물군집 분석)

  • Kim, Yo-Hwan;Lim, Jong-Hui;An, Chang-Hwan;Jung, Byung-Kwon;Kim, Sang-Dal
    • Journal of Applied Biological Chemistry
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    • v.55 no.1
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    • pp.47-53
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    • 2012
  • Increasing concerns over green farming technology, plant growth promoting rhizobacterium (PGRP) having growth promoting as well as plant disease suppressing properties was recently preferred to use for biological control of plant pathogens infecting plant. We measured the influence of the selected microbial consortium agents-a mixture of PGPR strains-, commercial bio-fungicide, and chemical pesticides on soil microbial community in red pepper field. The activities of soil enzyme such as dehydrogenase, urease, phosphatase, ${\beta}$-glucosidase, and cellulase were analyzed to investigate that of soil microbial community. We also measured plant length, main stem, stem diameter, number of branches and yields of red-pepper in order to observe the red pepper growth promotion. The results of measuring enzyme activities were dehydrogenase 3.5584 ${\mu}g$ TPF $g^{-1}h^{-1}$, urease 15.8689 ${\mu}g$ $NH_4{^-}N$ $g^{-1}h^{-1}$, phosphatase 0.5692 ${\mu}g$ PNP $g^{-1}h^{-1}$, ${\beta}$-glucosidase 2.4785 ${\mu}g$ PNP $g^{-1}h^{-1}$, and cellulase 86.1597 ${\mu}g$ glucose $g^{-1}h^{-1}$ in the soil treated with the microbial consortium agents, so it came out to be very active in the soil. Observing the growth of red-peppers, the main-stem length and the stem diameter were 6.1% and 8.1% higher in the soil treated with the selected microbial consortium agent than the chemical pesticides. After harvesting, yields were 7.3% higher in the soil treated with selected microbial consortium agents than the chemical pesticides. These results showed that microbial consortium agents contribute to increasing soil microbial diversity, growth promoting, and yield of red pepper.

Effects of Amitriptyline and Imipramine on Superoxide Generation, Myeloperoxidase Release, Leukotriene $B_4$ in Human Neutrophils (Amitriptyline과 Imipramine이 호중구에서의 Superoxide 생성, Myeloperoxidase 유리, Leukotriene $B_4$생성과 칼슘 동원에 나타내는 영향)

  • Shin Yong-Kyoo;Lee Chung-Soo;Lee Kwang-Soo
    • The Korean Journal of Pharmacology
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    • v.31 no.1 s.57
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    • pp.123-133
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    • 1995
  • A number of tricyclic antidepressants appear to have inhibitory action on calmodulin. Although amitriptyline, imipramine and doxepine have been shown to inhibit calcium uptake, oxidative phosphorylation and ATPase activities, effects of amitriptyline, imipramine and doxepine on functional responses of human neutrophils have not been elucidated. In this study, effects amitriptyline, imipramine and doxepine on superoxide and hydrogen peroxide generation, myeloperoxidase release, leukocriene B4 formation and intracellular calcium level were investigated. Superoxide and hydrogen peroxide production in heat aggregated IgG-activated neutrophils were inhibited by amitriptyline, imipramine and doxepine. EDTA, EGTA, verapamil and bepredil inhibited heat aggregated IgG-induced superoxide production. Chlorpromazine, trifluoperazine, staurosporine and H-7 also inhibited it. PMA-induced superoxide production was inhibited by amitriptyline, imipramine, doxepine, chlorpromazine and H-7. Amitriptyline, imipramine, chlorpromazine and trifluoperazine inhibited the myeloperoxidase release by heat aggregated IgG. Productions of $LTB_4$, and 5-HETE in heat aggregated IgG-activated neutrophils were inhibited by amitriptyline, imipramine and doxepine. In neutrophils, elevation of intracellular calcium induced by heat aggregated IgG was inhibited by amitriptyline, imipramine, doxepine, chlorpromazine and EGTA, while verapamil slightly inhibited increase of intracellular calcium and H-7 did not inhibit it. These results suggest that the inhibitory effect of amitriptyline, imipramine and doxepine on respiratory burst, myeloperoxidase release and LTB4 production in heat aggregated IgG-activated neutrophils appears to be ascribed to the inhibition of calcium mobilization, calmodulin and protein kinase C.

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Monitoring for optimum antioxidant extraction condition of Gugija (Lycium chinensis Mill) extract (구기자 추출물의 최적 항산화 추출조건 모니터링)

  • Kim, Hak-Yoon;Lee, Gee-Dong
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.451-460
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    • 2017
  • This study optimized the extraction of antioxidants from Gugija (Lycium chinensis Mill). To determine operational parameters, including ethanol concentration ($X_1$, 0~80%) and extraction time ($X_2$, 1~5 hr), response surface methodology was applied to monitor yield, anthocyanins, flavonoids and DPPH radical scavenging activity. Coefficients of determinations ($R^2$) of the models were range of 0.8645~0.9859 (p<0.01~0.1) in dependant parameters. Yield of Gugija extracts was maximized 23.12% in extraction conditions of 4.22 h at 8.25% ethanol. Anthocyanins was maximized 1.43 (OD in 530 nm) in extraction conditions of 3.06 h at 79.98% ethanol. Flavonoids was maximized $3,100{\mu}g/100g$ in extraction conditions of 3.37 h at 67.02% ethanol. DPPH radical scavenging activity was maximized 96.93% in extraction conditions of 1.67 h at 69.81% ethanol. Optimum extraction conditions (2.5 h extraction at 70% ethanol) were obtained by superimposing the contour maps with regard to anthocyanins, flavonoids and DPPH radical scavenging activity of Gugija. Maximum values of anthocyanins, flavonoids and DPPH radical scavenging activity in optimum extraction condition were 1.0080 (OD in 530 nm), $3,145{\mu}g/100g$, 96.96%, respectively. But values of anthocyanins, flavonoids and DPPH radical scavenging activity in water extraction condition (1 h at water) were 0.4652 (OD in 530 nm), $1,633{\mu}g/100g$, 86.98%, respectively.

Isolation of a New Herbicidal Compound Angelicin from Curly Dock (Rumex crispus L.) (소리쟁이(Rumex crispus L.)로부터 신규 살초활성물질 Angelicin의 분리)

  • Cho, Nam-Kyu;Lee, Sa-Eun;Choi, Jung-Sup;Hwang, Ki-Hwan;Koo, Suk-Jin;Wang, Hai-Ying;Kim, Song-Mun
    • Korean Journal of Weed Science
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    • v.30 no.3
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    • pp.183-190
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    • 2010
  • The objective of this study was to isolate a herbicidally active compound from curly dock (Rumex crispus), a native weed in Korea and to identify its' chemistry. The $GR_{50}$ value of methanol extracts which is determined by a seed bioassay using rapeseed (Brassica napus) seedlings was $935\;{\mu}g\;g^{-1}$. Activity-directed isolation of ethylacetate extract led to the isolation of ECDA fraction with $GR_{50}$ value $53\;{\mu}g\;g^{-1}$. Based on data of GC-MS, $^1H$-NMR and $^{13}C$-NMR, the chemical structure of ECDA was determined as 2H-furo[2,3-H]-[1]-benzopyran-2-one which is known as angelicin. The $GR_{50}$ values of angelicin to barnyardgrass (Echinochloa crus-galli), southern crabgrass (Digitaria ciliaris), and indian jointvetch (Aeschynomene indica) were 426, 66 and $216\;{\mu}g\;g^{-1}$, respectively. Our results suggest that angelicin could be used as a lead compound for the development of new herbicides.