The Korean Journal of Pharmacology (대한약리학회지)
- Volume 31 Issue 1 Serial No. 57
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- Pages.123-133
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- 1995
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- 0377-9459(pISSN)
Effects of Amitriptyline and Imipramine on Superoxide Generation, Myeloperoxidase Release, Leukotriene $B_4$ in Human Neutrophils
Amitriptyline과 Imipramine이 호중구에서의 Superoxide 생성, Myeloperoxidase 유리, Leukotriene $B_4$ 생성과 칼슘 동원에 나타내는 영향
- Shin Yong-Kyoo (Department of Pharmacology, School of Medicine, Chung-Ang University) ;
- Lee Chung-Soo (Department of Pharmacology, School of Medicine, Chung-Ang University) ;
- Lee Kwang-Soo (Department of Pharmacology, School of Medicine, Chung-Ang University)
- Published : 1995.06.01
Abstract
A number of tricyclic antidepressants appear to have inhibitory action on calmodulin. Although amitriptyline, imipramine and doxepine have been shown to inhibit calcium uptake, oxidative phosphorylation and ATPase activities, effects of amitriptyline, imipramine and doxepine on functional responses of human neutrophils have not been elucidated. In this study, effects amitriptyline, imipramine and doxepine on superoxide and hydrogen peroxide generation, myeloperoxidase release, leukocriene B4 formation and intracellular calcium level were investigated. Superoxide and hydrogen peroxide production in heat aggregated IgG-activated neutrophils were inhibited by amitriptyline, imipramine and doxepine. EDTA, EGTA, verapamil and bepredil inhibited heat aggregated IgG-induced superoxide production. Chlorpromazine, trifluoperazine, staurosporine and H-7 also inhibited it. PMA-induced superoxide production was inhibited by amitriptyline, imipramine, doxepine, chlorpromazine and H-7. Amitriptyline, imipramine, chlorpromazine and trifluoperazine inhibited the myeloperoxidase release by heat aggregated IgG. Productions of
삼환계 항우울제들은 calmodulin 억제 작용을 갖고 있으며, 칼슘 유입, 산화성 인산화 반응 및 ATPase 활성도를 억제하는 것으로 제시되고 있지만 사람 호중구에서의 기능 표현에 대한 효과는 밝혀져 있지 않다. 본 연구에서는 amitriptyline, imipramine과 doxepine이 superoxide와