Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Monitoring for optimum antioxidant extraction condition of Gugija (Lycium chinensis Mill) extract

구기자 추출물의 최적 항산화 추출조건 모니터링

  • Kim, Hak-Yoon (Faculty of?Environmental Science, Keimyung University) ;
  • Lee, Gee-Dong (Division of Integrated Biotechnology, Joongbu University)
  • Received : 2017.07.16
  • Accepted : 2017.08.29
  • Published : 2017.09.30
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Abstract

This study optimized the extraction of antioxidants from Gugija (Lycium chinensis Mill). To determine operational parameters, including ethanol concentration ($X_1$, 0~80%) and extraction time ($X_2$, 1~5 hr), response surface methodology was applied to monitor yield, anthocyanins, flavonoids and DPPH radical scavenging activity. Coefficients of determinations ($R^2$) of the models were range of 0.8645~0.9859 (p<0.01~0.1) in dependant parameters. Yield of Gugija extracts was maximized 23.12% in extraction conditions of 4.22 h at 8.25% ethanol. Anthocyanins was maximized 1.43 (OD in 530 nm) in extraction conditions of 3.06 h at 79.98% ethanol. Flavonoids was maximized $3,100{\mu}g/100g$ in extraction conditions of 3.37 h at 67.02% ethanol. DPPH radical scavenging activity was maximized 96.93% in extraction conditions of 1.67 h at 69.81% ethanol. Optimum extraction conditions (2.5 h extraction at 70% ethanol) were obtained by superimposing the contour maps with regard to anthocyanins, flavonoids and DPPH radical scavenging activity of Gugija. Maximum values of anthocyanins, flavonoids and DPPH radical scavenging activity in optimum extraction condition were 1.0080 (OD in 530 nm), $3,145{\mu}g/100g$, 96.96%, respectively. But values of anthocyanins, flavonoids and DPPH radical scavenging activity in water extraction condition (1 h at water) were 0.4652 (OD in 530 nm), $1,633{\mu}g/100g$, 86.98%, respectively.

본 연구는 구기자 항산화 성분의 최적 추출과 항산화 활성의 변화를 모니터링 하였다. 건조 구기자의 추출 조건은 에탄올 농도($X_1$, 0~80%) 및 추출 시간($X_2$, 1~5 hr)이며, 종속변수로는 수율, 안토시아닌, 플라보노이드 및 DPPH 라디컬 소거능으로 반응표면분석을 실시하였다. 가용성 고형분 수율, 안토시아닌, 플라보노이드 및 DPPH 라디컬 소거능에 대한 회귀식의 $R^2$은 각각 0.9066, 0.9859, 0.8645, 0.9464로 1~10%의 유의수준에서 유의성이 인정되었다. 건조 구기자 가용성 고형분 수율이 가장 높은 추출 조건은 에탄올 농도 8.25%에서 4.22 hr 추출한 것(23.12%)으로 나타났다. 안토시아닌이 가장 높은 추출 조건은 에탄올 농도 79.98%에서 3.06 hr 추출한 것(흡광도 1.43)으로 나타났다. 플라보노이드의 가장 높은 추출 조건은 에탄올 농도 67.02%에서 3.37 h 추출한 것($3,100{\mu}g/100g$)으로 나타났다. 그리고 DPPH 라디컬 소거능이 가장 높은 추출 조건은 에탄올 농도 69.81%에서 1.67 h 추출한 것(96.93%)으로 나타났다. 안토시아닌, 플라보노이드 및 DPPH 라디컬 소거능에 대한 등고선도를 겹쳐 그려 얻은 최적 조건(에탄올 농도 70% 및 추출 시간 2,5 hr)으로 추출된 추출물의 안토시아닌은 1.0080(흡광도) 이었으며, 플라보노이드 함량은 $3,145{\mu}g/100g$, 그리고 DPPH 라디컬 소거능은 97%로써 증류수로 1시간 추출한 대조구(안토시아닌 0.4652(흡광도), 플라보노이드 $1,633{\mu}g/100g$ 및 DPPH 라디컬 소거능 87%)에 비해 높은 항산화 성분 추출 및 항산화 효과를 나타내었다.

Keywords

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