• Weed & Turfgrass Science
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• v.4 no.4
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• pp.279-287
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• 2015

The present research was carried out in order to investigate the occurrence of problematic weed species in Cnidium officinale Makino. Field in South Korea. Total 53 sites of the 3 different regions in S. Korea were investigated from May to October, 2014. In Cnidium officinale fields, the identified weeds were distributed in 35 families and 99 species. Total 5 communities that consist of Commelina communis, Eleocharis kuroguwai, Persicaria vulgaris, Chenopodium album-Acalypha australis, and Galinsoga ciliata dominated the appearance. The weeds occurred in Cnidium officinale fields were divided into three groups in principal component plot analysis (PCA). It was observed that in control weeds plots; 20 plants of Cnidium officinale fresh weight is 739.9 g while the uncontrolled plots have no Cnidium officinale plants. The current investigation could be useful for estimation of future weeds occurrence, weed flora dynamics and establishment of weed control methods in Cnidium officinale fields in Korea.

• Journal of Life Science
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• v.26 no.10
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• pp.1163-1168
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• 2016

Plant growth-promoting rhizobacteria (PGPR) have gained worldwide importance and acceptance due to their agricultural benefits. These microorganisms are potential tools for sustainable agriculture, with effects on plant growth, biofertilization, induced systemic resistance, and biocontrol of plant pathogens. In this study, four different Pantoea species were isolated from field soil, and their plant growth-promoting characteristics were studied. Based on 16S rDNA gene sequencing analyses, the se were grouped into Pantoea ananatis, Pantoea citrea, Pantoea dispersa, Pantoea vagans and named as Pa1, Pc1, Pd1, Pv1, respectively. All of these strains have their ability for solubilization of insoluble phosphate depending on pH decrease at the range around pH 5 at 1days after inoculation and production of plant hormone indole acetic acid (IAA) with 85.3±16.3 μg/ml of Pa1, 183.9±16.8 μg/ml of Pc1, 28.8±17.3 μg/ml of Pd1 and 114.1±16.5 μg/ml of Pv1, respectively. Pa1, Pc1 and Pd1 also have high activity for production of gibberellin (GA₃) hormone with 331.1±19.2 μg/ml of Pa1, 288.5±16.8 μg/ml of Pc1, 309.2±18.2 μg/ml of Pd1, but Pv1 does not. Furthermore, all these species have significantly promoted the growth of the lettuce seedling plants at the range around 32~37% for fresh weight and 10~15% for shoot length enhancement, so that these microbe could be used as a potential bio-fertilizer agents.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.461-466
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• 2009

This study was carried out to evaluate the effects of yam (Dioscorea batatas Dence) extracts on the cell viability, growth and nucleus-DNA damage of tobacco cells which were exposed to $\gamma$-radiation stress. The viability and growth of tobacco cells exposed to 20 Gy of radiation stress were effectively recovered by pretreatment of 10 mg/L ethylacetate (EtOAc) yam extract. Pretreatment of EtOAc extract showed 20% higher cell viability and fresh weight growth than that of cells without pretreatment in 20 Gy radiation treated tobacco cells. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from tobacco cells. The T/H ratio of control-cells and treated-cells at 20 Gy were 1.05 and 1.68, and % head DNA of those cell were 86.7 and 71.3%, respectively, suggesting that nuclei of tobacco cells were severely damaged in the integrity of DNA by the treatment of $\gamma$-radiation. However, pretreatment of MeOH, EtOAc and n-BuOH extracts decreased radiation induced DNA-damage in the tobacco cells, showing T/H ratio of 1.37, 1.01 and 1.10 and % head DNA of 81.5, 87.6 and 88.7%, respectively.

• Food Science of Animal Resources
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• v.26 no.3
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• pp.349-355
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• 2006

A total of 120 pigs (Berkshire) were used to investigate the effect of dietary supplementation with artificial culture medium ot wild ginseng (CMWG) on the fatty acid composition, amino acid composition and sensory characteristics or pork About $60{\pm}3kg$ pigs were randomly assigned to one of four experimental diet groups[(both sexes)${\times}$(C: commercial diet feed; T: commercial diet+1 L CMWG per day for 70 days)]. Pigs were slaughtered at approximately 110 kg live weight, and fatty acid composition, amino acid composition and sensory characteristics were measured in pork loin. The monounsaturated and saturated fatty acid contents was greater in barrow fed a diet containing CMWG than those of the gilt pork and control groups, however the polyunsaturated fatty acid composition decreased. The EAA (essential amino acid) content was lower in pigs fed diets containing CMWG than that of the control groups, whereas the SAA (amino acid with sulfide) and FRAA (fragrant amino acid) contents were higher. The EAA and FAA (amino acid in relation to flavor) contents were lower for harrow than for gilt, however the SAAA (amino acid in relation to saccarinity), SAA and FRAA the contents were higher. Regarding the sensory evaluation of fresh meat, the color of gilt pork increased with diets containing CMWG relative to the control group. Drip loss and the marbling score for pigs fed with diets containing CMWG were higher in barrow than in gilt. The flavor and overall acceptability of cooked meat from the control group was higher for barrow than for silt.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.170-175
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• 1998

This study was conducted to determine the effect of seminal sections of tuber and staking methods on growth and yield of a chinese yam (Dioscorea batatas Decne). Tuber was divided into top head, head, median and tail which were used as seminal sections of tuber. The emergence of shoots was delayed in median and tail sections by 15 days as compared with top head and head sections. Sections from head and tail exhibited yield increase as high as 16 and 15%, respectively, compared to top head sections. Although flowering and bubil setting occurred almost at the same time in all staking methods, fresh weight of stem and leaves was higher in ${\wedge}-$, I-type stake supporting net than in the conventional stake. In the cultivar, 'Dan-ma', tuber yield didn't show a meaningful increase statistically by all staking methods. But ${\wedge}-$ and I-type stake supporting net increased the yield of the cultivar, 'Jang-ma', as high as 11 and 8%, respectively, compared to conventional stake. Also I-type stake was ideal supportor of net, considering the cost of management.

• Journal of the Korean Applied Science and Technology
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• v.6 no.1
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• pp.41-49
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• 1989

This study was carried out to study influence of antioxidants on serum and tissue of rats fed with thermally oxidized oil. The experimental animals used 30male rats of sprague-dawley weighting $150{\pm}25g$. They are divided into 5 groups and fed to experimental diets which are composed of 15％ thermally oxidized oil in addition to ascorbic acid, Vitamin E, Ethylendintrioteraaceticacid(EDTA) and none added oxidized oil by heat and fresh soybean oil group. Thermally oxidized oil was prepared from the soybean oil by heating at $180^{\circ}C$ for 30 hours. After feeding for 4 weeks, the result are as follows; 1. Body weight gain were lower B diet group than A diet group. 2. Total cholesterol levels in serum of all experimental diet groups except B diet group were higher than that of A diet group. 3. HDL-cholesterol levels of all experimental diet groups except E diet group were lower than that of A diet group. 4. The activities of GOT, GPT in serum of all experimental diet groups except B diet group and D diet group were higher than that of A diet group. 5. Vitamin E levels in serum of E diet group were highest than that of all experimental diet groups, and Vitamin E levels in liver of A diet group were highest than that of all experimental diet groups. 6. Lipid peroxide in Serum were highest B diet group than that of all experimental diet groups and the other experimental diet group significantly lower than that of the A diet group. 7. Lipid peroxide in liver of all experimental diet group except E diet group were significantly higher than that A diet group and lipid peroxide in kidney of all experimental diet group except B diet group were lower than that of A diet group. Four these results, as Vitamin C, Vitamin E, and EDTA added diets have effect of thermally oil by antioxidants, it could be suggested that thermally soybean oil diet has required to add antioxidant because it has not sufficient Vitamin E for antioxidant and intake and overtake level of thermally soybean oil diet should be studied to go ahead.

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.4
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• pp.116-124
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• 2015

The objective of this study is to evaluate the effects of the replacement levels of grinded fly-ash on the repaired mortar based on a polymer. The main parameters are the curing temperature and replacement levels of grinded fly-ash. The curing temperature and the replacement levels of grinded fly-ash are varied at $40^{\circ}C$, $20^{\circ}C$ and $5^{\circ}C$, and between 0% and 35% of the total binder by weight, respectively. The flow in fresh mortar and compressive strengths according to ages, the relationship of stress-strain, elastic modulus and modulus rupture in hardened mortar, as well as scanning the electron microscopy and the X-ray diffraction of mortar, were measured, respectively. The test results showed that the flow, elastic modulus and modulus rupture are great in mortar specimens with 20~30% of the replacement levels of grinded fly-ash. In addition, compressive strengths according to ages were affected by the replacement levels of grinded fly-ash and the curing temperature indicated that the strength development ratio of mortar with 20% of the replacement levels of grinded fly-ash was greater than others. In the prediction of the compressive strength specified by the ACI 209 code, the strength development at an early and late age can be generalized by the functions of the replacement levels of grinded fly-ash and the curing temperature. In the analysis of scanning the electron microscopy and the X-ray diffraction, the number and intensity of peaks increased and the form of CSH gels on the surface of the particle of grinded fly-ash was observed.

• Journal of Ginseng Research
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• v.35 no.1
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• pp.31-38
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• 2011

In order to distinguish the cultivation area of Panax ginseng, principal component analysis (PCA) using quantitative and qualitative data acquired from HPLC was carried out. A new HPLC method coupled with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous quantification of ten major ginsenosides, namely $Rh_1$, $Rg_2$, $Rg_3$, $Rg_1$, Rf, Re, Rd, $Rb_2$, Rc, and $Rb_1$ in the root of P. ginseng C. A. Meyer. Simultaneous separations of these ten ginsenosides were achieved on a carbohydrate analytical column. The mobile phase consisted of acetonitrile-water-isopropanol, and acetonitrile-water-isopropanol using a gradient elution. Distinct differences in qualitative and quantitative characteristics for ginsenosides were found between the ginseng roots produced in two different Korean cultivation areas, Ganghwa and Punggi. The ginsenoside profiles obtained via HPLC analysis were subjected to PCA. PCA score plots using two principal components (PCs) showed good separation for the ginseng roots cultivated in Ganghwa and Punggi. PC1 influenced the separation, capturing 43.6% of the variance, while PC2 affected differentiation, explaining 18.0% of the variance. The highest contribution components were ginsenoside $Rg_3$ for PC1 and ginsenoside Rf for PC2. Particularly, the PCA score plot for the small ginseng roots of six-year old, each of which was light than 147 g fresh weight, showed more distinct discrimination. PC1 influenced the separation between different sample sets, capturing 51.8% of the variance, while PC2 affected differentiation, also explaining 28.0% of the variance. The highest contribution component was ginsenoside Rf for PC1 and ginsenoside $Rg_2$ for PC2. In conclusion, the HPLC-ELSD method using a carbohydrate column allowed for the simultaneous quantification of ten major ginsenosides, and PCA analysis of the ginsenoside peaks shown on the HPLC chromatogram would be a very acceptable strategy for discrimination of the cultivation area of ginseng roots.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.106-112
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• 2015

The objective of this study was to examine the growth reaction of strawberry plants to the mixed red and blue LED sources and their installation method. The artificial light sources were : LED PAR(PPFD $2{\sim}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), LED BAR(PPFD $100{\sim}120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and incandescent(PPFD $2{\sim}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) lamp. The lighting treatment was started at the first cluster flowering period as a night breaking lighting and was applied during 3 hours, between 22:00 and 01:00 every day. Plant height and leafstalk length were longer in plants treated with incandescent lamp, where as fresh and dry weight of shoot were heavier in LED PAR compared to incandescent lamp treatment. LED PAR treatment also resulted in the largest leaf area, chlorophyll content was increased by $0.36mg{\cdot}g^{-1}$ after 60 days from the starting of the artificial lighting. According to the experimental results application of 16W LED PAR lamps and W-type installation method can improve light environment in strawberry lighting culture.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.311-319
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• 2011

Overusing chemical fertilizers involves potassium accumulation in the soil, which can become a toxicity problem in agriculture. This study was conducted to investigate the effect of potassium (K) treatment on growth, physiological characteristics, and morphological changes using Chinese cabbage (Brassica rapa L. ssp. campestris). With high (600 mM) K treatment, the plant growth traits of leaf length, leaf area, and fresh and dry weight of shoots and roots decreased, whereas chlorophyll content increased. As the concentration of K increasing, total N, P, and K increased in leaves, but concentrations of Ca, Mg, and Na decreased. However, Mn, Fe and Zn contents were highest in 100 mM K treatment. Chlorophyll a, b, and carotenoids increased with increasing K concentration. Maximum photochemical efficiency ($F_v/F_m$) was not significant in the all treatments, whereas $CO_2$ assimilation decreased with increasing K level due to stomatal degradation. Total free amino acids increased with the 10 and 100 mM K but decreased at 600 mM K treatments. Therefore, the growth and physiological characteristics of Chinese cabbage ascertained that tolerance up to 100 mM K when grown with nutrient solution in pot culture.