• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 춘계학술발표대회
• /
• pp.263-266
• /
• 2000

The purified biosurfactant $3.16g/{\ell}$ was obtained after cultivation for 104hr at $25^{\circ}C$ with an optimal agitation speed of 200rpm, an aeration rate of 2vvm in a $14{\ell}$ fermenter containing $5.5{\ell}$ of LB medium and 1%(w/v) olive oil as a carbon source. For the kinetic studies, the optimal substrate concentration was analyzed on different olive oil concentrations(0.1, 0.5, 1.0, 1.5, 2.0%(w/v)) and optimal culture conditions(MLBM, 200rpm, 2vvm at $25^{\circ}C$) in a $14{\ell}$ jar fermenter. The results obtained indicate that $K_s$=0.0086 $g/{\ell}$, $q_s$= 0.664 $g/g{\cdot}h$, $q_p$= $4.2{\times}10^{-3}$ $g/g{\cdot}h$, and ${\mu}_{max}$ was determined as $0.1449h^{-1}$.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 춘계학술발표대회
• /
• pp.275-278
• /
• 2000

본 연구에서 비스판균에 비해 아포형성능이 우수한 변이주를 선별하고, 발효조 배양에서 활성아포 생산성의 증가를 확인하였다. 최종 선별된 변이주 KD21균주를 5 L jar fermenter에서 배양한 결과, 36시간만에 총균수는 $5.5{\times}10^9$ CFU/mL에 도달하고, 최대 활성아포수는 $2.7{\times}10^9$ CFU/mL에 도달하였다. 이는 비스판 친균주에 비해 활성아포의 생산성이 77%증가하였음 확인 할 수 있었다. 장질환 치료제로 판매되고 있는 비스판 제제의 활성아포 생산성이 우수한 변이주를 획득함으로서 친균주에 비해 가격경쟁력을 가질수 있을것으로 판단된다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.378-381
• /
• 2001

Motierella alpina DSA-12 의 회 분식 발효에서 $MnSO_4$ 첨가로 작은 pellet을 형성하게 하여 유가식 배양으로의 적용 가능성을 보았으나, $KH_2PO_4_4$의 경우에는 균체증식에는 효과가 있었지만, 균체가 풀어져 자라 점도가 증가하여 ARA 의 합성이 억제되었다. 유가식 배양에 14 % 암모니아 용액을 사용할 때 균체증식과 총지질의 함량에 영향이 있음을 확인했으며, 62.1 g/L의 균체량에 60% 이상의 지질 함량으로 12.0 g/L 의 아라키돈산을 생산할 수 있었다.

• Korea-Australia Rheology Journal
• /
• 제14권1호
• /
• pp.11-16
• /
• 2002

Cephalosporium acremonium is a filamentous microorganism producing cephalosporin C. The morphological differentiation of C. acremonium in submerged culture is closely related with the rheological properties of culture broth and production of cephalosporin C. In this study, the rheological and morphological properties of culture broth of C. acremonium were investigated. In the seed broths of shake-flask and fermenter culture, the Herschel-Berkley equation was in excellent agreement with experimental results in the whole range of shear rate. In the seed broths of shake-flask culture, morphological differentiation into arthrospores affected to changes of apparent viscosity. But results in the fermenter culture, morphological factors such as mean hyphal thickness and the number of tips gave more effect on changes of apparent vitacosity than differentiation into arthrospores. Overall, it suggested that the morphological parameters measured by image analysis can be used as a good parameter to indicate the rheological properties of culture broth of C. acremonium M25.

• Journal of Microbiology and Biotechnology
• /
• 제28권2호
• /
• pp.293-297
• /
• 2018

Controlling the residual glucose concentration is important for improving productivity in $\text\tiny{L}$-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio ($R_{C/N}$) of glucose and ammonia water was predetermined via a stoichiometric approach, on the basis of glucose-ammonia water consumption rates. In a 5-L fermenter, 102 g/l $\text\tiny{L}$-threonine was obtained using our glucose-ammonia water combined feeding strategy, which was then successfully applied in a 500-L fermenter (89 g/l). Therefore, we conclude that an automatic combination feeding strategy is suitable for improving $\text\tiny{L}$-threonine production.

• 한국물환경학회지
• /
• 제18권4호
• /
• pp.435-440
• /
• 2002

A pilot scale SBR (effective volume, $20m^3$) for the treatment of piggery wastewater treatment was performed with three different kinds of wastewater; fermenter effluent, scraper type and slurry type. The react phase in SBR was performed by sub-cycle operation consisting of repeated short cycle of anoxic-aerobic step. The fermenter effluent was characterized by the rapid nitrification and $NO_X-N$ accumulation due to depletion of organic matter in wastewater. The scraper type wastewater showed appropriate nitrogen removal efficiency, however, a poor response capacity for high loading rate often resulted in increased nitrogen concentration in effluent. Moreover, severe P release was the most serious problem in scraper type wastewater. SBR treated slurry type wastewater with high nitrogen removal efficiency to satisfy effluent quality requirement. It was thought that high concentration of organic matter in slurry made it possible to uptake P during SBR operation, where P concentration of 140mgP/l was decreased to 8mgP/l. As results, SBR was suitable to treat slurry type wastewater which has been discharged to the ocean till now.

• KSBB Journal
• /
• 제18권5호
• /
• pp.418-423
• /
• 2003

발효조의 냉각수 공급 제어 신호와 냉각수의 유출입 지점의 온도를 온라인으로 측정하여 이 값들로부터 냉각량을 추정할 수 있는 실험식을 얻었다. 회분식 배양을 통하여 냉각량이 온라인으로 측정될 수 있음을 확인하였고 기질의 재투입시 냉각량의 변화를 통하여 유가배양의 제어변수로 활용할 수 있음을 점검하였다. 미리 프로그램된 목표 냉각량의 증가곡선에 따라 냉각량이 증가되면서 세포가 자라도록 유가배양을 실시하였다. 본 연구를 통하여 냉각량의 온라인 측정방법을 제시하였고 이를 유가배양 제어변수로 활용하여 세포의 성장속도를 조절할 수 있음을 보였다.

• 동아시아식생활학회지
• /
• 제2권1호
• /
• pp.83-94
• /
• 1992

As the result of the research on the manufacturing procedure of Sogog-ju in the classical literature(35 kinds), it was found that Sogog-ju is usually brewed on the first Boar day of January and Sogog-ju base is added to it in about 7days and then the fermentation of Sogog-ju comes to end in about 21 days. The material grains out of 48 items, used in the first step of brewing Sogog-ju, are nonglutnous rice 47 items(98%), flour 20items(42%), somnuruk 9 items(19%) out of nuruk (fermenter) 48 items (100%) and nuruk water 5 items(17%), and flour 3 items(6%) are used, but fermenter nuruk is not used. When the grains are used in the first step of brewing, they are usually in the form of gruel and rice cake, and in the second step of brewing, they are usually made into steam water soaked rice. Nuruk, Which is largely used in the first step, is usually put after the processed grains get cold, but in the second step of brewing, it may be put into the still warm grains. There are hardly any cases when nuruk is used in conspicously small quantity.

• Journal of Microbiology and Biotechnology
• /
• 제17권2호
• /
• pp.195-201
• /
• 2007

This study investigated the effects of various culture parameters (carbon sources, temperature, initial pH of culture, NaCl concentration, and light) on the growth and canthaxanthin production by Dietzia natronolimnaea HS-1. The results showed that the most effective carbon source for growth and canthaxantin production was glucose, and the best pH and temperature were 7 and $31^{\circ}C$, respectively. In addition, the biomass and canthaxanthin production increased in a medium without NaCl and in the presence of light. Under the optimized conditions, the maximum biomass, total carotenoid, and canthaxanthin production were $6.12{\pm}0.21g/l,\;4.51{\pm}0.20mg/l,\;and\;4.28{\pm}0.15mg/l$, respectively, in an Erlenmeyer flask system, yet increased to 7.25 g/l, 5.48 mg/l, and 5.29 mg/l, respectively, in a batch fermenter system.

• 생명과학회지
• /
• 제12권3호
• /
• pp.242-249
• /
• 2002

본 연구는 채소류 모잘록병균인 R. solani AG4에 길항하는 미생물 B. ehimensis YJ-37의 500$m\ell$ 플라스크 배양, 5 $\ell$ jar fermentor 배양, 2,000 $\ell$ 대형탱크의 배양 조건 및 길항물질 생성능을 조사하였다. 500$m\ell$ 플라스크 배양에서의 길항물질 최적생산조건은 1.5% starch, 0.6% peptone, 0.3% $Na_2$HP $O_4$, 0.15% MgC1$_2$, pH 8.0, 배양온도 32$^{\circ}C$, 배양시간 54시간으로 나타났고, 이때 미생물의 수는 1.42$\times$$10^{ 8}$ cfu/$m\ell$21g-DCW/$\ell$), 항진균 활성은 13.9mm로 나타났다. 5$\ell$ jar fermenter 배양에서의 길항물질 최적생산조건은 플라스크배양에서의 최적 생산조건에서 공기주입량과 교반속도를 변화시켜 조사한 결과, 공기주입량 2 vvm, 교반속도 200 rpm, 배양시간 48시간 이후 미생물의 수와 균체의 량은 2.06 $\times$ $10^{8}$ cfu/$m\ell$ 및 30g-DCW/$\ell$, 항진균 활성은 13.4 mm로 나타났다. 2,000 $\ell$ 대형탱크 배양에서는 교반속도 200 rpm, 산소주입량 30 vvm으로 고정하고 10일간 배양하여 미생물의 생육 및 길항물질 생성능을 조사한 결과는 미생물의 수와 균체의 량은 0.81 $\times$ $10^{8}$ cfu/$m\ell$와 12g-DCW/$\ell$, 항진균 활성은 8.6mm로 나타나 5$\ell$ jar fermenter 배양에 배해 균의 수는 1/10로 줄었으며 항진균 활성도 64%로 낮게 나타났다.다.