• Microbiology and Biotechnology Letters
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• v.49 no.1
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• pp.111-119
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• 2021

A bacterial strain isolated from a Malva verticillata leaf was identified as Bacillus velezensis MV2 based on the 16S rRNA sequencing results. Complete genome sequencing revealed that B. velezensis MV2 possessed a single 4,191,702-bp contig with 45.57% GC content. Generally, Bacillus spp. are known to produce diverse antimicrobial compounds including bacteriocins, polyketides, and non-ribosomal peptides. Antimicrobial compounds in the B. velezensis MV2 were extracted from culture supernatants using hydrophobic interaction chromatography. The crude extracts showed antimicrobial activity against both gram-positive bacteria and gram-negative bacteria; however, they were more effective against gram-positive bacteria. The extracts also showed antifungal activity against phytopathogenic fungi such as Fusarium fujikuroi and F. graminearum. In time-kill assays, these antimicrobial compounds showed bactericidal activity against Bacillus cereus, used as indicator strain. To predict the type of antimicrobial compounds produced by this strain, we used the antiSMASH algorithm. Forty-seven secondary metabolites were predicted to be synthesized in MV2, and among them, fourteen were identified with a similarity of 80% or more with those previously identified. Based on the antimicrobial properties, the antimicrobial compounds may be nonribosomal peptides or polyketides. These compounds possess the potential to be used as biopesticides in the food and agricultural industry as an alternative to antibiotics.

• Economic and Environmental Geology
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• v.54 no.3
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• pp.373-387
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• 2021

In this study, the impact of clustered groundwater usage facilities and the proper amount of groundwater usage in the Daejeong-Hangyeong watershed of Jeju island were evaluated based on the data-driven analysis methods. As the applied data, groundwater level data; the corresponding precipitation data; the groundwater usage amount data (Jeoji, Geumak, Seogwang, and English-education city facilities) were used. The results show that the Geumak usage facility has a large influence centering on the corresponding location; the Seogwang usage facility affects on the downstream area; the English-education usage facility has a great impact around the upstream of the location; the Jeoji usage facility shows an influence around the up- and down-streams of the location. Overall, the influence of operating the clustered groundwater usage facilities in the watershed is prolonged to approximately 5km. Additionally, the appropriate groundwater usage amount to maintain the groundwater base-level was analyzed corresponding to the precipitation. Considering the recent precipitation pattern, there is a need to limit the current amount of groundwater usage to 80%. With increasing the precipitation by 100mm, additional groundwater development of approximately 1,500m³-1,900m³ would be reasonable. All the results of the developed data-driven estimation model can be used as useful information for sustainable groundwater development in the Daejeong-Hangyeong watershed of Jeju island.

• Journal of Life Science
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• v.31 no.5
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• pp.488-495
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• 2021

The aim of this study was to investigate the possible antioxidant effect of Spirodela polyrhiza (SP) on rats fed a high fat and high cholesterol diet supplemented with either 5% (SPA group) or 10% (SPB group) SP for 4 weeks. The hepatic SOD activity of the HF group significantly decreased compared to that of the N group, but that of the SPA and SPB groups significantly increased. The GPx activity of the SPA and SPB groups in the liver was significantly greater than that of the HF group, and the hepatic catalase activity of the SPA and SPB groups significantly increased compared to the HF group. The hepatic superoxide radical content of the mitochondria and microsomes of the HF group significantly increased compared to that of the N group, but the contents were reduced in the group that took SP powder. The hepatic hydrogen peroxide content in the cytosol and mitochondria of the SP powder group was lower than in the HF group. The carbonyl content in the mitochondria and microsomes of the SPA and SPB groups was significantly lower than in the HF group. The TBARS values in the liver significantly decreased in the SPA and SPB groups. Spirodela polyrhiza was thus effective in reducing oxidative stress by regulating the hepatic antioxidant enzymes and the free radicals in rats fed high fat and high cholesterol diets.

• Journal of Food Hygiene and Safety
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• v.36 no.3
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• pp.228-238
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• 2021

The aim of this research was to develop a rapid and easy multi-residue method for determining dimethipin, omethoate, dimethipin, chlorfenvinphos and azinphos-methyl in agricultural products (hulled rice, potato, soybean, mandarin and green pepper). Samples were prepared using QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) and analyzed using gas chromatography-tandem mass spectrometry (GC-MS/MS). Residual pesticides were extracted with 1% acetic acid in acetonitrile followed by addition of anhydrous magnesium sulfate (MgSO₄) and anhydrous sodium acetate. The extracts were cleaned up using MgSO₄, primary secondary amine (PSA) and octadecyl (C₁₈). The linearity of the calibration curves, which waas excellent by matrix-matched standards, ranged from 0.005 mg/kg to 0.3 mg/kg and yielded the coefficients of determination (R²) ≥ 0.9934 for all analytes. Average recoveries spiked at three levels (0.01, 0.1, 0.5 mg/kg) and were in the range of 74.2-119.3%, while standard deviation values were less than 14.6%, which is below the Codex guideline (CODEX CAC/GL 40).

• Journal of The Korean Association For Science Education
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• v.41 no.1
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• pp.33-46
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• 2021

Inquiry occupies an important place in science education, and research related to inquiry is widely conducted. However, due to the inclusiveness of the concept of "exploration," each researcher perceives its meaning differently, and approaches may vary. In addition, criticisms have been raised that the results of classes using inquiry in science education do not guarantee meaningful changes to students. Therefore, this study attempts to identify the trend of SSCI-level research papers dealing with inquiry in science education over the past three years to confirm the current status and effectiveness of the inquiry. Researches used in the analysis are International Journal of Science Education, Journal of Research in Science Teaching, Research in Science Education, and Science Education, and limited to those that directly suggest "inquiry (enquiry)" as a keyword. Based on extracted 75 papers, the classification process was conducted, and an analysis frame was derived inductively by reflecting the subject and characteristics. Specific cases for each category were presented by dividing into three aspects: perception and perspective on inquiry, support and strategy for inquiry, and teacher professional development for inquiry. The results of examining the implications for scientific inquiry are as follows: First, rather than defining inquiry as an implicit proposition or presenting it as a step-by-step procedure, it was induced to grasp the meaning of inquiry more comprehensively and holistically. Second, as to whether the inquiry-based instruction is effective in all aspects of the cognitive, functional, and affective domains of science, the limitations are clearly presented, and the context-dependent and subject-specific properties and limitations of inquiry are emphasized. Third, uncertainty in science inquiry-based instruction can help learners to begin their inquiry and develop interest, but in the process of recognizing data and restructuring knowledge, explicit and specific guidance and scaffolding should be provided at an appropriate timing.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.518-527
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• 2020

The aim of this study was to define the toxic effects of diuron and irgarol, which are new-antifouling agents, on the fertilization rate and normal embryogenesis rate in the sea urchin, Hemicentrotus pulcherrimus. In addition, the study was intended to confirm the hindrance of development in sea urchins. The fertilization rate of H. pulcherrimus was not decreased by the tested concentrations. However, the normal embryogenesis rate was decreased in a concentration-dependent manner. The 50% effective concentrations (EC₅₀) of normal embryogenesis rate were 7.12 mg L^-1 and 2.31 mg L^-1, respectively. As the embryos developed into pluteus larvae, after 18 h of exposure to diuron and irgarol at EC₅₀, development of the early gastrula stage was delayed, and significant developmental delays were observed after 24 h. After this, continuous developmental delays were observed in the process leading to the early gastrular, gastrular, early pluteus, and pluteus stages. Therefore, the toxic effects of diuron and irgarol on sea urchins were attributed to the delay in the developmental processes in the early life stages. Diuron and irgarol are highly persistent in the environment and have known-well toxic effects on various marine organisms including invertebrates, as shown in this study. Therefore, it is urgent to establish an environmental protection strategy to prevent the pollution of and preserve the marine environment.

• Journal of agriculture & life science
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• v.53 no.4
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• pp.19-28
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• 2019

This study was conducted to investigate the effect of nutrient solution strength and duration of nutrient starvation on the growth and development of strawberry (Fragaria x ananassa Duch.) 'Maehyang' and 'Sulhyang' at the flowering stage. Cuttings of runner plants were stuck on November 23th, 2017 and were covered with a layer of black plastic film to block light from penetrating and keep the relative humidity high. The black plastic film was removed after 16 days and rooted plants were cultivated for one month with irrigation of water. The Yamazaki nutrient solution with an electrical conductivity (EC) of 1.85 or 3.71 dS·m-1 (1x or 2x ionic strength, respectively) and pH 5.55 was fed to plants after either 0 (control), 1, 3 or 5 weeks of nutrient starvation to the end of experiment. Plant height in both cultivars decreased gradually with the increase in duration of nutritional starvation. The earlier the nutritional starvation started, the smaller the shoot fresh weight of 'Maehyang'. Hence the greatest shoot fresh weight was obtained in the control which was supplied with the nutrient solution continuously. Shoot fresh weight of 'Sulhyang' was the greatest in 1x ionic strength and one week of nutrient starvation before planting. Although number of flowers on the first flower cluster of 'Maehyang' and 'Sulhyang' showed no significant differences, 'Maehyang' had the greatest number of flowers in the 2x ionic strength solution and one week of nutrient starvation before planting, while 'Sulhyang' had that in the 1x ionic strength treatment. These results suggest that it is considered effective to supply a nutrient solution at a low concentration for a short period of time for increasing the number of flower differentiated on the first flower cluster in both cultivars.

• Korean journal of applied entomology
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• v.59 no.4
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• pp.295-301
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• 2020

In this study, we aimed to investigate the changes in the catch rate of Monochamus saltuarius, based on sexual maturation by using aggregation-sex pheromone traps. Ovariole development of caught M. saltuarius females was compared to that of the ones not caught using traps. In a mesh cage set up at the Hongneung experimental forest, we placed a multi-funnel trap with or without an aggregation-sex pheromone lure. M. saltuarius adults, which emerged from pine logs, were grouped in four according to the emergence dates (0, 1, 7, and 10 days after emergence [DAE]). We released beetles into the mesh cage to investigate the catch rate using the traps. In each group, a total of 80 beetles (20 beetles × 4 replications) were tested, making it a total of 320 beetles. Among the four groups, M. saltuarius adults in the 7 DAE group were caught more frequently using the traps, especially with a pheromone lure; the other groups showed a low catch rate. A similar number of female and male beetles were caught using the traps. Regarding ovariole development, all the female adults in the 0 and 1 DAE groups were immature, while those in the other two groups were completely developed. Therefore, aggregation-sex pheromone traps might have a limitation in the prevention of pine wilt disease because of the transmission of pine wood nematode during maturation feeding of newly emerged M. saltuarius adults. However, aggregation-sex pheromone traps can be effective for collecting sexually mature M. saltuarius adults, for the investigation of seasonal occurrence of beetles in forests.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.6
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• pp.189-207
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• 2020

The government has achieved quantitative growth in university start-up education while supporting start-up education. However, it failed to systematize start-up education from an academic, policy, and practical perspective and to reveal the relationship between education and achievements in supporting start-ups. Therefore, there is a lack of interest and effort to promote effective education. In Europe, in-depth research has already been done over many years to establish an EntreComp system. Competences create values for others and attempt to apply them to education, viewing them as the people's lifelong competitiveness. On the other hand, it is urgent to improve the education system as domestic university start-up education is mainly focused on cultural level start-up skills and easy-to-access education from a business administration perspective. Based on this, the entrepreneurship competence-based start-up education system was designed. Next, eight EntreComp frames were drawn for university students through the Focus Group Interview (FGI) and Delphi survey methods, as well as domestic and international prior studies on EntreComp. In 2018, 919 start-up education programs of 42 start-up leading universities were conducted to derive the status of education by EntreComp. Prior studies of 25 entrepreneurship competences, including data from Bacigalupo et al.(2016), which studied EntreComp in the EU, were investigated and reflected the frequency of research and the importance of education and start-up perspectives. Based on the purpose of the university start-up education presented in this study, the entrepreneurship competence frame consisting of a total of eight, including spotting opportunities, value creation, self improvement, mobilising resources, technology application, strategic management, relationship, and learning through experience, was derived through expert verification. It also investigated the current status of education by competence, the degree of reflection of competence education, and the relationship with the results of support for start-ups that reflect the number of students enrolled in each university. Through this, it was suggested that future start-up education at universities could be improved from the EntreComp perspective. It has a differentiation in research in that it conducted a thorough survey using the data on start-up courses operated by leading startup universities for a certain period. However, it is difficult to generalize because the number of samples of leading startup universities is limited. Nevertheless, this study proposes the educational goal of advancing university start-up education from the perspective of entrepreneurial competence, cultivating future required competences, and fostering entrepreneurial talents that create value for others. In addition, it is meaningful in that it presents a clear direction for subsequent research by preparing a framework for research from a more essential perspective on the entrepreneurship competence frame.

• Korean Journal of Heritage: History & Science
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• v.54 no.1
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• pp.206-219
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• 2021

The warming of the climate is increasing the damage caused by termites to wooden buildings, cultural properties and houses. A group removal system can be installed around the building to detect and remove termite damage; however, if the site is not visited regularly, every one to two months, you cannot observe whether termites have spread within, and it is difficult to take prompt effective action. In addition, since the system is installed and operated in an exposed state for a long period of time, it may be ineffective or damaged, resulting in a loss of function. Furthermore if the system is installed near a cultural site, it may affect the aesthetic environment of the site. In this study, we created a detection system that uses wood, cellulose, magnets, and magnetic sensors to determine whether termites have entered the area. The data was then transferred to a low power LoRa Network which displayed the results without the necessity of visiting the site. The wood was made in the shape of a pile, and holes were made from the top to the bottom to make it easier for termites to enter and produce a cellulose sample. The cellulose sample was made in a cylindrical shape with a magnet wrapped in cellulose and inserted into the top of a hole in the wood. Then, the upper part of the wood pile was covered with a stopper to prevent foreign matter from entering. It also served to block external factors such as light and rainfall, and to create an environment where termites could add cellulose samples. When the cellulose was added by the termites, a space was created around the magnet, causing the magnet to either fall or tilt. The magnetic sensor inside the stopper was fixed on the top of the cellulose sample and measured the change in the distance between the magnet and the sensor according to the movement of the magnet. In outdoor experiments, 11 cellulose samples were inserted into the wood detection system and the termite inflow was confirmed through the movement of the magnet without visiting the site within 5 to 17 days. When making further improvements to the function and operation of the system it in the future, it is possible to confirm that termites have invaded without visiting the site. Then it is also possible to reduce damage and fruiting due to product exposure, and which would improve the condition and appearance of cultural properties.