• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.491-502
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• 2013

Persimmon (Diospyros kaki Thunb) fruit is one of the most important fruit and have been cultivated from ancient times in Korea. In this study, we found 16 EST-SSR markers that contained one or more EST-SSR sites from 246 cDNA sequences. The developing of EST-SSR marker analysis from 42 persimmon cultivars was compared by genetic relationships and morphological relationships using 6 qualitative traits (fruit related 6 traits) and 19 quantitative traits (flower related 19 traits). In this study, 25 primer sets were tested to identify PCR polymorphism and 14 potential EST-SSR primer pairs were selected. The result of morphological relationship EST-SSR marker analysis showed that the coefficient 0.02 was difficult to categorize in several groups. And then, coefficient 0.77 of genetic relationship showed that the group was classified as four groups. The result of correlation distance between genetic relationship and morphological relationship were investigated was low significance (-0.03). Our results also provided an optimized method for improvement of breeding efficiency and introduce of superior character at persimmon cultivars using EST-SSR markers which was useful for further investigation.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.04b
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• pp.1013-1016
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• 2002

제어 시스템에 사용되는 흐름 제어 언어로는 IL(Instruction List), ST(Structured Text), FBD(Function Block Diagram), SFC(Sequential Function Chart), LD (Ladder Diagram)가 있다. 일반적으로 제어 시스템에 탑재하여 사용하는 언어는 상기 언어 중 두 종류 이하의 특정 언어로 제한되어 있다. 이러한 제약을 보완하기 위해, 모든 흐름 제어 언어를 통합 분석할 수 있는 통합 분석기가 필요하다. 본 논문에서는 흐름 제어 언어의 통합 분석 처리가 가능하도록 그래픽 표현의 FBD 를 문자 표현의 EST(Extended Structured Text)로 변형하는 규칙과 문자 표현의 EST 를 IL 로 변형하는 규칙을 제시한다. 언어간의 변형 과정에서 FBD 를 ST 로 표현할 수 없는 부분을 EST 로 정의한다. 또한 본 논문에서 제안된 EST 를 기반으로 통합 분석기의 구조를 제시한다.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.116-122
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• 2011

In this study, we report the generation and analysis of 1,392 expressed sequence tags (ESTs) from Korean Stewartia (Stewartia koreana Nakai). A cDNA library was generated from the young leaf tissue and a total of 1,392 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. Finally, 1,301 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 100 nucleotides. A total of 893 unigene, consisting of 150 contigs and 743 singletons, was identified after assembling. Also, we identified 95 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 65% of ESTs were homologous with known function and 11.6% of ESTs were matched with putative or unknown function. The remaining 23.2% of ESTs showed no significant similarity to any protein sequences found in the public database. Annotation based searches against multiple databases including wine grape and populus sequences helped to identify putative functions of ESTs and unigenes. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Stewartia and provided for the useful tools as a genetic resource.

• Proceedings of the Korean Information Science Society Conference
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• 2002.10d
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• pp.634-636
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• 2002

산업분야에서 자동화 시스템은 자동설계, 생산설비의 관리, 품질검사 등 각종 생산과 관련되어 모든 일을 자동으로 처리 할 수 있도록 하여 생산성을 향상시킨다. 일반적으로 자동화 시스템에서 사용되는 소프트웨어는 사용할 수 있는 흐름 제어 언어가 2종류 이하로 제한이 되어 있고, 동일한 시스템에서 언어의 혼용을 통한 시뮬레이션이 불가능하다. 이에 본 논문에서 혼용 사용이 가능한 흐름 제어 언어 통합 분석기를 제시한다. 고급언어 형태의 ST를 기초로 확장한 언어인 EST를 제시하고 통합 분석기를 위하여 그래픽언어를 EST로 변환하고 흐름제어 규칙 작성이 가능한 FBD 편집기, FBD 편집기에서 출력한 EST를 다시 변환하는 EST-IL 변환기를 설계 및 구현한다. EST를 기준으로 그래픽 언어를 통합하는 통합 분석기는 흐름 제어 언어의 통합 시뮬레이션이 가능하다.

• Korean Journal of Ichthyology
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• v.18 no.4
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• pp.283-292
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• 2006

Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the kidney mRNA of olive flounder (Paralichthys olivaceus). In the survey of 390 ESTs chosen from the kidney cDNA library, 250 ESTs showed significant homology to previously described genes while 140 ESTs were unidentified or novel. Comparative analysis of the 250 identified ESTs showed that 14 (5.6%) clones were representing 11 unique genes identified as homologous to the previously reported olive flounder ESTs, 198 (79.2%) clones representing 160 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 38 (15.2%) clones representing 37 genes of known sequences with unknown functions. We also identified several kinds of immune associated proteins, indicating EST as a powerful method for identifying immunerelated genes of fish as well as identifying novel genes. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.257-265
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• 2007

We constructed a cDNA library of testis from olive flounder (Paralichthys olivaceus) and a total of 248 expressed sequence tag (EST) clones were generated. In order to understand the molecular compositions of the olive flounder testis organs, the expression profiles of the identified clones in the cDNA library were analyzed. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 248 EST clones, 156 ESTs showed significant homology to previously described genes while 92 ESTs were unidentified or novel. Comparative analysis of the 156 identified ESTs showed that 6 (3.8%) clones were representing 5 unique genes identified as homologous to the previously reported olive flounder ESTs, 100 (64.1%) clones representing 94 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 50 (32.1%) clones representing 44 genes of known sequences with unknown functions. Furthermore, the testis library showed a more even distribution of cDNA clones with relatively fewer abundant clones that tend to contribute redundant clones in EST projects; thus, the testis library can supply more unique and novel cDNA sequences in olive flounder EST project.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.465-470
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• 2002

This study presents results of electro-phoretically detectable isozyme variation in Crossing Block (CB) lines of two-rowed barley maintained by the National Crop Experiment Station. The specific objectives were to determine allelic frequencies at the four Est loci(Est1, Est2, Est4, and Est5) and their distribution over 380CB lines of two-rowed barley. A total of 17 alleles were detected over the four Est loci in these lines. There were 4 alleles (Pr, Al, Ca, and Af at the Est1 locus and their frequencies were 69.7, 1.1, 28.4, and 0.8%, respectively. At the Est2 locus, 5 different alleles (Dr, Fr, Sp, Un and a recessive null allele) were detected and their frequencies were 2.9,84.5,0.5,2.1, and 10%, respectively. four alleles (Nz, Su, At, and null were detected at the Est4 locus and the allelic frequency of Su was about 84%. Four alleles(Mi, Pi, Te, and a null allele(od)) were detected at the Est5 locus and their frequencies were 34.2, 61.0, 2.4, and 2.4%, respectively. Based on the allelic frequencies over the four Est loci, 380 CB lines were classified into 25 genotypes. The most frequent genotypes were G1(Pr-Fr-Su-Mi) and G2(Pr-Fr-Su-Pi), and their frequencies were 28.1 and 39.5%, respectively. The frequencies of other genotypes were less than 10%.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.7
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• pp.3582-3588
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• 2013

Expressed sequence tag (EST) library was constructed from A. tamarense. Base sequences of EST clones were analyzed and saxitoxin biosynthesis-related genes were cloned. Sequences of 827 clones were analyzed and 564 EST were functionally clustered using Blast searches against GenBank. Main genes in the EST had functions on cellular organization, cell metabolism, energy, cell cycle and DNA processing, cellular transport and transport, cell rescue, defense, death and aging, and transcription. Moreover, expression of S-adenosylmethionine synthetase and H2A histone family genes were increased in the toxic A. tamarense. These results show that two genes could be a good biomarkers for the detection of saxitoxin biosynthesis in the A. tamarense.

• The KIPS Transactions:PartD
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• v.11D no.2
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• pp.415-422
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• 2004

Automation systems improve the productivity of works which relate to product design, facilities management, fault processing and quality evaluation. In these systems, the description language for monitoring and control process is called new control language. These are five flow control languages : IL, ST, FBD, SFC and LD. IL and ST are based on left form. FBD, SFC and LD are based on graphic form. Generally, a software which monitors and controls a system is allowed to use just one flow control language. It is impossible to use more than two languages for simulation in the same system environment. In this paper, we analyzed the characteristics of flow control languages and the process of programming in the legacy system. In addition, for the Integrated processing of languages, we propose Extended ST based on the high-level ST language. Based on this research, we implement a graphical language editor and EST-IL convertor. The graphical language editor makes sequence rules, and converts graphical language into EST. EST-IL convertor has a function to convert EST into IL which is similar to assembly language. As the result of this paper, we present a scheme which integrates all the flow control language processing based on IL.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.5B
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• pp.459-473
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• 2008

This study investigated typhoon characteristics that provoke precipitation which is much attacking periodically in our country, and calculated probability precipitation of extreme rainfalls using Empirical Simulation Technique. The typhoon influenced in Korea was happened 3.18 times per, and year exposed to affect Korea during 107 hours. The depth of precipitation with the typhoon was different according to observation points. The extreme precipitation of typhoon events has analyzed by change and trend analyses. In the results, mean and standard deviation of extreme rainfall has been increasing than the past events in some areas. Also, About 143 typhoons influenced Korea was applied in EST techniques using center position, central pressure, time precipitation data using rainfall observatory in Korea. Therefore, we applied EST techniques and calculated probability precipitation. In the results, Jeonla-do, Gyeongsang-do and Gangwon-do will have heavy rain with typhoon events in high probability.