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Genotypic Variation of Esterase Isozyme in Breeding Lines of Two-rowed Barley by Electrophoretic Banding Pattern  

박광근 (작물시험장)
최홍집 (경북농업기술원)
이종호 (국립종자관리소)
서세정 (작물시험장)
김재철 (작물시험장)
남중현 (작물시험장)
김상효 (농업과학기술원)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.47, no.6, 2002 , pp. 465-470 More about this Journal
Abstract
This study presents results of electro-phoretically detectable isozyme variation in Crossing Block (CB) lines of two-rowed barley maintained by the National Crop Experiment Station. The specific objectives were to determine allelic frequencies at the four Est loci(Est1, Est2, Est4, and Est5) and their distribution over 380CB lines of two-rowed barley. A total of 17 alleles were detected over the four Est loci in these lines. There were 4 alleles (Pr, Al, Ca, and Af at the Est1 locus and their frequencies were 69.7, 1.1, 28.4, and 0.8%, respectively. At the Est2 locus, 5 different alleles (Dr, Fr, Sp, Un and a recessive null allele) were detected and their frequencies were 2.9,84.5,0.5,2.1, and 10%, respectively. four alleles (Nz, Su, At, and null were detected at the Est4 locus and the allelic frequency of Su was about 84%. Four alleles(Mi, Pi, Te, and a null allele(od)) were detected at the Est5 locus and their frequencies were 34.2, 61.0, 2.4, and 2.4%, respectively. Based on the allelic frequencies over the four Est loci, 380 CB lines were classified into 25 genotypes. The most frequent genotypes were G1(Pr-Fr-Su-Mi) and G2(Pr-Fr-Su-Pi), and their frequencies were 28.1 and 39.5%, respectively. The frequencies of other genotypes were less than 10%.
Keywords
esterase isozyme; barley; starch gel electrophoresis; genetic variation;
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