Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Expressed Sequence Tag Analysis of Toxic Alexandrium tamarense and Identification of Saxitoxin Biosynthetic Genes

독성 Alexandrium tamarense 의 EST 분석 및 삭시톡신 생합성 유전자의 확인

  • Chang, Man (Marine Ecosystem Research Division, Korea Institute of Ocean Science and Technology) ;
  • Lee, Juyun (Marine Ecosystem Research Division, Korea Institute of Ocean Science and Technology) ;
  • Chung, Youngjae (Department of Life Science and Biotechnology, Shin Gyeong University) ;
  • Lee, Gunsup (South Sea Environment Research Division, Korea Institute of Ocean Science and Technology) ;
  • Kim, Dongguin (Department of Biological Science, Silla University) ;
  • Lee, Taek-Kyun (South Sea Environment Research Division, Korea Institute of Ocean Science and Technology)
  • 장만 (한국해양과학기술원 해양생태계연구부) ;
  • 이주연 (한국해양과학기술원 해양생태계연구부) ;
  • 정영재 (신경대학교 생명공학과) ;
  • 이건섭 (한국해양과학기술원 남해특성연구부) ;
  • 김동균 (신라대학교 생명과학과) ;
  • 이택견 (한국해양과학기술원 남해특성연구부)
  • Received : 2013.03.19
  • Accepted : 2013.07.11
  • Published : 2013.07.31
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Abstract

Expressed sequence tag (EST) library was constructed from A. tamarense. Base sequences of EST clones were analyzed and saxitoxin biosynthesis-related genes were cloned. Sequences of 827 clones were analyzed and 564 EST were functionally clustered using Blast searches against GenBank. Main genes in the EST had functions on cellular organization, cell metabolism, energy, cell cycle and DNA processing, cellular transport and transport, cell rescue, defense, death and aging, and transcription. Moreover, expression of S-adenosylmethionine synthetase and H2A histone family genes were increased in the toxic A. tamarense. These results show that two genes could be a good biomarkers for the detection of saxitoxin biosynthesis in the A. tamarense.

A. tamarense로부터 ESTs library를 제작하였다. 이들의 염기서열을 분석하여 STX 생합성 관련 유전자를 클로닝하였다. 연구결과 827 클론의 염기서열이 분석되었고, 564개의 EST가 GenBank에서의 Blast search를 사용하여 기능에 따라 분류되었다. EST에서의 주요 유전자는 cellular organization, cell metabolism, energy, cell cycle과 DNA processing, cellular transport와 transport, cell rescue, defense, death와 aging 및 transcription 등으로 분석되었다. 특히 S-adenosylmethionine synthetase와 H2A histone family 유전자의 발현이 독성 A. tamarense에서 증가하였다. 이러한 결과는 두 개의 유전자가 A. tamarense에서의 삭시톡신 생합성을 검출하기 위한 좋은 바이오마커가 될 수 있음을 보여준다.

Keywords

References

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