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Expressed Sequence Tags of Expression Profiles of Olive Flounder (Paralichthys olivaceus) Testis  

Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Jong-Hyun (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Noh, Jae Koo (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Hyun Chul (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Young-Ok (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Kim, Woo-Jin (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Kim, Kyu-Won (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Kim, Kyung-Kil (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Publication Information
Korean Journal of Ichthyology / v.19, no.4, 2007 , pp. 257-265 More about this Journal
Abstract
We constructed a cDNA library of testis from olive flounder (Paralichthys olivaceus) and a total of 248 expressed sequence tag (EST) clones were generated. In order to understand the molecular compositions of the olive flounder testis organs, the expression profiles of the identified clones in the cDNA library were analyzed. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 248 EST clones, 156 ESTs showed significant homology to previously described genes while 92 ESTs were unidentified or novel. Comparative analysis of the 156 identified ESTs showed that 6 (3.8%) clones were representing 5 unique genes identified as homologous to the previously reported olive flounder ESTs, 100 (64.1%) clones representing 94 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 50 (32.1%) clones representing 44 genes of known sequences with unknown functions. Furthermore, the testis library showed a more even distribution of cDNA clones with relatively fewer abundant clones that tend to contribute redundant clones in EST projects; thus, the testis library can supply more unique and novel cDNA sequences in olive flounder EST project.
Keywords
Expressed sequence tags (ESTs); expression profile; olive flounder (Paralichthys olivaceus); testis;
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