Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Expressed Sequence Tags of Expression Profiles of Olive Flounder (Paralichthys olivaceus) Testis

ESTs (Expressed Sequence Tags)를 통한 넙치(Paralichthys olivaceus) 정소의 유전자 발현 패턴 분석

  • Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Jong-Hyun (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Noh, Jae Koo (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Hyun Chul (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Young-Ok (Biotechnology Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Woo-Jin (Biotechnology Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Kyu-Won (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kim, Kyung-Kil (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
  • 이정호 (국립수산과학원 육종연구센터) ;
  • 김종현 (국립수산과학원 육종연구센터) ;
  • 노재구 (국립수산과학원 육종연구센터) ;
  • 김현철 (국립수산과학원 육종연구센터) ;
  • 김영옥 (국립수산과학원 생명공학연구소) ;
  • 김우진 (국립수산과학원 생명공학연구소) ;
  • 김규원 (서울대학교 약학대학) ;
  • 김경길 (국립수산과학원 육종연구센터)
  • Received : 2007.10.01
  • Accepted : 2007.11.22
  • Published : 2007.12.31
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Abstract

We constructed a cDNA library of testis from olive flounder (Paralichthys olivaceus) and a total of 248 expressed sequence tag (EST) clones were generated. In order to understand the molecular compositions of the olive flounder testis organs, the expression profiles of the identified clones in the cDNA library were analyzed. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 248 EST clones, 156 ESTs showed significant homology to previously described genes while 92 ESTs were unidentified or novel. Comparative analysis of the 156 identified ESTs showed that 6 (3.8%) clones were representing 5 unique genes identified as homologous to the previously reported olive flounder ESTs, 100 (64.1%) clones representing 94 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 50 (32.1%) clones representing 44 genes of known sequences with unknown functions. Furthermore, the testis library showed a more even distribution of cDNA clones with relatively fewer abundant clones that tend to contribute redundant clones in EST projects; thus, the testis library can supply more unique and novel cDNA sequences in olive flounder EST project.

본 연구에서는 넙치(Paralichthys olivaceus) 정소에 대한 cDNA library를 제작하여 총 248개의 EST (Expressed sequence tag)를 분석을 하였다. 넙치 정소의 유전자 발현 패턴을 조사하기 위하여 염기서열의 유사성 분석을 한 결과 248개의 EST 중 156개의 EST는 이미 밝혀진 유전자와 유사성이 있는 것으로 나타났으며, 92개의 EST는 새로운 유전자로 밝혀졌다. 유전자의 기능이 밝혀진 250개의 EST 중 6개(3.8%)의 EST는 이미 알려진 넙치 EST와 상동성이 있는 유전자로 확인되었고, 100개(64.1%)의 EST는 다른 생물에서 알려진 유전자와 상동성이 있는 것으로 나타났다. 그러나 50개(32.1%)의 EST는 전혀 기능이 알려지지 않은 새로운 유전자로 밝혀졌다. 이상의 결과에서 넙치 정소에서 발현되는 유전자는 다른 조직에 비해 일부 유전자의 상대적인 발현정도가 많지 않고, 대부분의 유전자가 골고루 발현함으로써 다양한 유전자의 발현패턴이 확인되었다. 따라서 넙치 정소에 대한 cDNA library는 특이하고 새로운 발현 유전자의 탐색에 좋은 재료로 사용될 것으로 추측된다.

Keywords

Acknowledgement

Supported by : National Fisheries Research and Development Institute

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