KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Genotypic Variation of Esterase Isozyme in Breeding Lines of Two-rowed Barley by Electrophoretic Banding Pattern

전기영동 패턴에 의한 2조보리 계통의 Esterase 동위효소 유전자형 변이분석

  • Published : 2002.12.01
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Abstract

This study presents results of electro-phoretically detectable isozyme variation in Crossing Block (CB) lines of two-rowed barley maintained by the National Crop Experiment Station. The specific objectives were to determine allelic frequencies at the four Est loci(Est1, Est2, Est4, and Est5) and their distribution over 380CB lines of two-rowed barley. A total of 17 alleles were detected over the four Est loci in these lines. There were 4 alleles (Pr, Al, Ca, and Af at the Est1 locus and their frequencies were 69.7, 1.1, 28.4, and 0.8%, respectively. At the Est2 locus, 5 different alleles (Dr, Fr, Sp, Un and a recessive null allele) were detected and their frequencies were 2.9,84.5,0.5,2.1, and 10%, respectively. four alleles (Nz, Su, At, and null were detected at the Est4 locus and the allelic frequency of Su was about 84%. Four alleles(Mi, Pi, Te, and a null allele(od)) were detected at the Est5 locus and their frequencies were 34.2, 61.0, 2.4, and 2.4%, respectively. Based on the allelic frequencies over the four Est loci, 380 CB lines were classified into 25 genotypes. The most frequent genotypes were G1(Pr-Fr-Su-Mi) and G2(Pr-Fr-Su-Pi), and their frequencies were 28.1 and 39.5%, respectively. The frequencies of other genotypes were less than 10%.

맥주보리 교배모본(Crossing block) 380품종 및 계통을 공시재료로 esterase 동위효소 가운데 4개의 loci에 대한 allele의 종류, 발견빈도, 유전자형 등을 분석한 결과는 다음과 같다. 1. 조사한 380 계통중 Est1 locus에서는Pr, Al, Ca, Af등 4개의 alleles이 있는 것으로 관찰되었으며 그 중 Pr allele이 약 70%, Ca allele이 28.4%로 대부분을 차지하였고, Ca 및 기타 Al allele은 2% 미만이었다. 2. Est2 locus에서는 Dr, Fr, Sp, Un, null 등 5개의 allele이 있는 것으로 나타났고 allele이 84.5%로 가장 높은 비율을 차지하였고, null allele가 10%이었다. 3. Est4 locus에서는 Nz, Su, At, null 등 4개의 allele이 발견되었는데 Su allele가 약 84%로 대부분을 차지하였으며, Nz, allele가 10.5%, At allele가 4.2%의 빈도를 보였다. 4. Est5 locus에서는 Mi, Pi, Te, od(null) 등 4개의 allele이 발견되었으며 Pi allele이 61.0% Mi allele이 34.2%이었다. 5. 4개의 Esterase loci에서 나타나는 pattern을 기준으로 한 유전자형은 25가지의 유형으로 분류할 수 있었으며, G1형(Pr-Fr-Su-Mi)이 28.1%, G2형(Pr-Fr-Su-Pi)이 39.5%로 대부분을 차지하였고 다음으로 12형(Ca-Fr-Su-Pi)이 약 8.1%의 비율로 나타났다. 기타의 유전자형들은 그 발견 빈도가 극히 낮은 편이었다.

Keywords

References

  1. Backes G., G. Fischbeck, A. Graner and A. Jahoor. 1993. Localization of agronomically important traits in baley(Hordeum vulgare L.) by means of RFLP marker. Barley Genetics Newsletter 23 : 60-64
  2. Devaux R, and M. Zivy. 1994. Protein markers for anther culturability in barley. Theoretical Applied Genetics 88 : 701-706 https://doi.org/10.1007/BF01253973
  3. Fedak G. 1974. Allozymes as aids to Canadian barley cultivar identifi-cation, Euphytica 23 :166-173 https://doi.org/10.1007/BF00032757
  4. Gouis L. J., M. Erdogan, W. Fredt and F. Ordon. 1995. Potential and limitation of isozymes for chromosomal location of resistance genes against barley mild mosaic virus(BaMMV). Euphytica 82 : 25-30 https://doi.org/10.1007/BF00028706
  5. Kahler A. L. and R. W. Allard. 1970. Genetics of isozyme variants in barley. 1. Esterases. Crop Sci. 10 : 444-448 https://doi.org/10.2135/cropsci1970.0011183X001000040039x
  6. K-ahler A. L. 1981. Worldwide patterns of genetic variation among four esterase loci in barley(Hordeum vulgare L.). Theoretical Applied Genetics 59:101-111
  7. Konishi T. 1989. 同位靜素からみた大麥の遺情的分化に關する硏究. 同山大學資源生物科學脚究所 硏究成果報告書
  8. Konishi T., K. Abe, S. Matsuura and Y. Yano. 1990. Distorted Segregation of the esterase isozyme genotypes in barley(Hordeum vuIgare L.). Japan J. Genet. 65 : 411-416 https://doi.org/10.1266/jjg.65.411
  9. Konishi T, N. Kawada, H. Yoshida and K. Sohtone. 1989. Linkage relationship between two loci for the barley yellow mosaic resis- tance of Mokusekko 3 and esterase isozymes. Japan. J. Breed. 39 : 423-430 https://doi.org/10.1270/jsbbs1951.39.423
  10. Nielsen G. and H. B. Johansen. 1986. Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isozyme loci of 47 reference varieties. Euphytica 35 : 717-728 https://doi.org/10.1007/BF00028579
  11. Ohmori T, Murata M. and F. Motoyoshi. 1995. RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato. Japan. J. Genet. 10: 179-184
  12. Son E. R., Lee Y. S., Yoon K. E, and Y. W. Ha. 1985. Appropriate elec- trophoresis techniques and isozymes to identification of barley Cul- tivars. Korean J. Crop Sci. 30(4): 405-411