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Immune Gene Discovery by Expressed Sequence Tags Generated from Olive Flounder (Paralichthys olivaceus) Kidney  

Lee, Jeong-Ho (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Young-Ok (Biotechnology Research Center, National Fisheries Research and Development Institute)
Kim, Jong-Hyun (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Noh, Jae Koo (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Hyun Chul (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Kyung-Kil (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Kim, Kyu-Won (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Publication Information
Korean Journal of Ichthyology / v.18, no.4, 2006 , pp. 283-292 More about this Journal
Abstract
Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the kidney mRNA of olive flounder (Paralichthys olivaceus). In the survey of 390 ESTs chosen from the kidney cDNA library, 250 ESTs showed significant homology to previously described genes while 140 ESTs were unidentified or novel. Comparative analysis of the 250 identified ESTs showed that 14 (5.6%) clones were representing 11 unique genes identified as homologous to the previously reported olive flounder ESTs, 198 (79.2%) clones representing 160 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 38 (15.2%) clones representing 37 genes of known sequences with unknown functions. We also identified several kinds of immune associated proteins, indicating EST as a powerful method for identifying immunerelated genes of fish as well as identifying novel genes. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.
Keywords
expressed sequence tags (ESTs); expression profile; olive flounder (Paralichthys olivaceus); kidney; immune gene;
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