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http://dx.doi.org/10.5762/KAIS.2013.14.7.3582

Expressed Sequence Tag Analysis of Toxic Alexandrium tamarense and Identification of Saxitoxin Biosynthetic Genes  

Chang, Man (Marine Ecosystem Research Division, Korea Institute of Ocean Science and Technology)
Lee, Juyun (Marine Ecosystem Research Division, Korea Institute of Ocean Science and Technology)
Chung, Youngjae (Department of Life Science and Biotechnology, Shin Gyeong University)
Lee, Gunsup (South Sea Environment Research Division, Korea Institute of Ocean Science and Technology)
Kim, Dongguin (Department of Biological Science, Silla University)
Lee, Taek-Kyun (South Sea Environment Research Division, Korea Institute of Ocean Science and Technology)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.14, no.7, 2013 , pp. 3582-3588 More about this Journal
Abstract
Expressed sequence tag (EST) library was constructed from A. tamarense. Base sequences of EST clones were analyzed and saxitoxin biosynthesis-related genes were cloned. Sequences of 827 clones were analyzed and 564 EST were functionally clustered using Blast searches against GenBank. Main genes in the EST had functions on cellular organization, cell metabolism, energy, cell cycle and DNA processing, cellular transport and transport, cell rescue, defense, death and aging, and transcription. Moreover, expression of S-adenosylmethionine synthetase and H2A histone family genes were increased in the toxic A. tamarense. These results show that two genes could be a good biomarkers for the detection of saxitoxin biosynthesis in the A. tamarense.
Keywords
Alexandrium tamarense; Biomarker; Biosynthetic genes; ESTs; Saxitoxin;
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