• Korean Journal of Microbiology
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• v.41 no.1
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• pp.81-86
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• 2005

One of the endoglucanases, F-I-III, was purified from the culture filtrate of T. sp. C-4 through procedures including chromatography on Sephacryl S-200, DEAE-Sepharose A-50, and Chromatofocusing on Mono-P (FPLC). The molecular weight of the enzyme was determined to be about 56,000 Da by SDS-PAGE, and pI of 4.9 by analytical isoelectric focusing. F-I-III showed the highest enzyme activity at $55^{\circ}C$, and the pH optimum of the enzyme was 5.0. There was no loss of activity when the enzyme was incubated at $50^{\circ}C$ for 24 hours. The specific activity of the enzyme F-I-III toward the CMC was 315.4 U/mg. The Km value for $PNPG_2$ of F-I-III was 2.69 mM. N-terminal sequence of F-I-III was analyzed to be QPGTSTPEVHPKKLTTYK. It showed $95\%$ of homology to that of EGI from T. reesei. The presence of some metal ions (1 mM) had only a little effect on CMCase activity. The treatment of the reducing agents resulted in the increase of endoglucanase activity.

• Journal of Convergence for Information Technology
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• v.10 no.2
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• pp.116-125
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• 2020

This study examined the effect of ego-resilience, function of family, stress of adapting to a new culture perceived by youth from multi-cultural families at Gyeonggi-do on their mental health. In doing so, the study sought to suggest effective supportive visits to enhance their mental health. For this, the study carried out a descriptive analysis, t-test, one-way anova, hierarchical regression analysis among 304 middle schoolers from multi-cultural families living at Gyeonggi-do. The study found following results. The higher youth from multi-cultural family perceived the procedural stress, sub-variable of stress of adapting to a new culture and the lower they perceived emotional control, sub-variable of ego-resilience, the higher depression and anxiety they experienced, resulting in worsening of their mental health state. Based on these findings, the study, in order to enhance the mental health of youth from multi-cultural family and their ego-resilience, discussed and suggested development of psychological/emotional programs, preparation of supportive measures to overcome their stress in the course of their cultural adaptation as well as systematic supplementation of related laws in support of multi-cultural families.

• Journal of Convergence for Information Technology
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• v.10 no.7
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• pp.185-193
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• 2020

This purpose of this study was to investigate the effects of 16-week long-term walking exercise on the feeling, mood state, and life stress of college students. Participations in this study were 50 college students, and 24 Walking Group and 26 Control Group were random sampled to experiment the psychological effect of exercise walking. The experimental design was designed by factorial design by 2×3 repeated measurements. Data collection was conducted for 0week, 8week, 16week of feeling, mood state and life stress of college students. As a result, WG showed a positive increase in feeling status compared to CG. Negative mood state such as tension, anger, depression, fatigue, and confusion have been greatly reduced, vigor has increased, and stress on college student life such as friends, study, economy, future, and values has decreased statistically. The results of this study can be used as a way of coping with the stress of college students to improve their study motivation and quality of life.

• Journal of Convergence for Information Technology
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• v.10 no.7
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• pp.58-66
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• 2020

The purpose of this study was to identify the relation of health behaviors and the quality of life by life cycle of hypertensive patients. This study was conducted by analyzing secondary data with data from the 2017 Community Health Survey. The subjects were total of 62,056 who were diagnosed with hypertension and analyzed using the SPSS WIN 20.0 program. As a result of the study, As a result of the study, first, hypertension treatment and drug compliance were significantly lower in adulthood than in middle-aged and older adults. Second, it was confirmed that the quality of life score by life cycle was the highest in adulthood and decreased as age increased. Third, the quality of life according to health behaviors differed according to drinking, exercise, and low-salt diet throughout the life cycle. Considering the results presented in this study, it is suggested that health education by life cycle is essential for lifelong health management of hypertensive patients. In particular, it is considered that a convergence approach such as education and medical approaches will be needed to develop measures to increase drug compliance in adult hypertensive patients and to have healthy health behaviors to improve quality of life.

• Journal of Convergence for Information Technology
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• v.10 no.7
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• pp.98-107
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• 2020

Job satisfaction is an important part of social workers who strive to improve the quality of life and independence of their clients. The goal of this study was to reveal the correlation between social workers' self-efficacy, job satisfaction, and supervision to determine whether supervision regulated the effect of social worker's self-efficacy on job satisfaction. In addition, the effect of social worker's self-efficacy on job satisfaction was grasped, and the control effect of supervision regarding the process of social worker's self-efficacy on job satisfaction was identified. For the study, 150 social workers at 10 community welfare centers in Gyeonggi-do and North Seoul area were surveyed, and the statistical program PASW (SPSS) 25.0 was used for data analysis. As a result of the study, it was confirmed that the social worker's self-efficacy had a significant effect on job satisfaction, and that the social worker's self-efficacy had a significant static effect on job satisfaction. In addition, in the process of social worker's self-efficacy affecting job satisfaction, the mediating effect of the supervision function (administrative, educational, and supportive) was found to be significant, so it turned out that the supervision function for social workers was crucial to enhance self-efficacy.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.324-331
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• 2020

This study used the financial information of 29 Regional public hospitals registered in the HASPA. Regional public hospitals analyzed the ratio of operating financing by management activities and how they affect net profit. The results of the study, Regional Public Hospitals ratio of fund financing for management activities was 83.50 percent for medical revenues, 9.53 percent for Incidental Revenue of Medical, 4.54 percent for Contribution Revenue, 4.42 percent for Other non-medical profits, 1.21 percent for depreciation Expense and 0.73 percent for Amount of reserve for unique purpose project. As a result of examining the effect of financing by management operation on net profit, the ratio of operating funds by medical revenue, by incidental revenue of medical, by contribution revenue and by other non-medical profits had a positive impact on net profit of Regional public hospitals. And It has been confirmed that the ratio of amount of reserve for unique purpose project has a negative impact on net profit. Therefore, it is necessary for Regional public hospitals to manage costs in consideration of revenues and internal reserves.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.168-176
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• 2020

Reactive oxygen species (ROS) damage DNA and cause cancer. Therefore, the research is being conducted on the development of antioxidants for the removal of ROS. This study was performed to investigate antioxidant activity and protective effect against oxidative DNA damage using ethyl acetate fractions from the cone of Pinus rigida × taeda (ERT). The antioxidant activity was evaluated using the DPPH, ABTS radical scavenging assay, reducing power assay, and Fe2+ chelating assay. Also, the contents of phenolic compounds and vitamin C related to antioxidant activity were analyzed to confirm phytochemicals. The DNA protective effect against oxidative stress was confirmed by the φX-174 RF I plasmid DNA cleavage assay. As a result, ERT showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. The results of reducing power and Fe2+ chelating activities were 77.32 ± 2.28% and 64.09 ± 1.01% at 200 ㎍/㎖. Also, ERT showed a DNA protective effect against oxidative stress.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.55-63
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• 2020

The IoT technology is a field that applies and converges the technologies in the existing industrial environment, instead of new technologies. The IoT technology is releasing various application services converged with other industries such as smart home, healthcare, construction, and automobile, and it is also possible to secure the work efficiency and convenience of users of IoT-based technologies. However, the security threats occurring in the IoT-based technology environment are succeeding to the vulnerability of the existing wireless network environment. And the occurrence of new and variant attacks in the combination with the ICT convergence environment, is causing damages. Thus, in the IoT technology-based environment, it would be necessary to have researches on the safe transmission of messages in the communication environment between user and device, and device and device. This thesis aims to design a safe communication protocol in the IoT-based technology environment. Regarding the suggested communication protocol, this thesis performed the safety analysis on the attack techniques occurring in the IoT technology-based environment. And through the performance evaluation of the existing PKI-based certificate issuance system and the suggested communication protocol, this thesis verified the high efficiency(about 23%) of communication procedure. Also, this thesis verified the reduced figure(about 65%) of the issued quantity of certificate compared to the existing issuance system and the certificate management technique.

• Journal of Convergence for Information Technology
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• v.10 no.1
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• pp.82-92
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• 2020

This study analyzed the factors affecting organizational adaptation among the members of SMEs. Until now, research on organizational adaptation has been mainly conducted in large corporations or public institutions. Very little research has been conducted on SME members. Factors affecting the organizational adaptation of employees were set by individual factors and job environment factors. In the individual factors, the sub-variables were set as self-efficacy, optimism, and resilience, and in the job environment factors, organizational fairness and organizational culture. The sample data were surveyed using a survey method such as visit, telephone call, e-mail and fax. 98 data were used for the analysis. Statistical analysis was performed using regression analysis. As a result, self-efficacy, optimism, and resilience of individual factors were analyzed as positive variables for organizational adaptation of employees, and organizational fairness in the job environment was analyzed as variables that did not positively affect organizational adaptation of employees. However, the organizational culture of the job environment was analyzed as a variable that positively affects organizational adaptation of employees. These results showed that the fair application of wage level, workload, compensation, and opinion reflection did not affect the organizational adaptation of employees. On the other hand, in the preceding studies, organizational adaptability was conducted mainly in large corporations, but this study was able to suggest practical implications in analyzing organizational adaptability among SME members.

• The Korea Journal of Herbology
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• v.25 no.2
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• pp.129-136
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• 2010

Objective : Sinapis alba L. (SA) is a korean traditional herbal medicine that is usually used to prevent or treat inflammatory diseases, such as respiratory infection and rheumatoid arthritis. However, the effects of SA supplementation in vitro on serum antibody levels, splenocyte and peritoneal macrophage immune responses have not yet been determined. In this study, we examined the effect of SA on the production of Th1/Th2 cytokines. Methods : Splenocytes were isolated from naive C57BL/6 mice. Cells were enriched for CD4+ cell populations by first staining the cells with anti-CD4 (BD PharMingen, Calif, USA). CD4+ T cells were selected on a (CS) column, and the flow-through was collected as CD4+ T cells. Isolated cells were activated by overnight incubation on 24-well plates coated with $1{\mu}g/mL$ anti-CD3, $1{\mu}g/mL$ anti-CD28 and with SA ($100{\mu}g/mL$). Primary macrophages were collected from the peritoneal cavities of mice (8-week-old female C57BL/6). The peritoneal macrophages were washed and plated with RPMI-1640 overnight for the experiments. After 48-hours cultures, samples were centrifuged at 2000 rpm for 10 minutes, and the supernatants were stored at $-80^{\circ}C$. Mouse IL-4, IFN-$\gamma$ and TNF-$\alpha$ were quantified using ELISA kits (BioSource International, Camarillo, Calif, USA) according to the manufacturer's protocols. Results : SA at 100ug/ml decreased the generation of Th1 cytokine (IFN-$\gamma$) by 0.5-fold. However, SA has no effect on Th2 (IL-4) production. Conclusions : These results suggest that SA may play an important role in the control of T-cell-mediated autoimmunity by down-regulation of Th1 cytokine (especially IFN-$\gamma$, TNF-$\alpha$). These data may contribute to the design of new immunomodulating treatments for a group of autoimmune diseases.