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http://dx.doi.org/10.22156/CS4SMB.2020.10.11.168

Antioxidant Activity and DNA Protective Effect against Oxidative Stress of Pinus rigida × taeda Cone  

Choi, Jisoo (Dept. of Pharmaceutical Science, Jungwon University)
Jang, Taewon (Dept. of Medicinal Plant Resources, Andong National University)
Min, Youngsil (Dept. of Pharmaceutical Science, JungWon University)
Lee, Manhyo (Hemp Promotion Project Team, Gyeongbuk Institute for Bio Industry)
Park, Jaeho (Dept. of Pharmaceutical Science & Institute of International Agricultural Research, JungWon Univ.)
Publication Information
Journal of Convergence for Information Technology / v.10, no.11, 2020 , pp. 168-176 More about this Journal
Abstract
Reactive oxygen species (ROS) damage DNA and cause cancer. Therefore, the research is being conducted on the development of antioxidants for the removal of ROS. This study was performed to investigate antioxidant activity and protective effect against oxidative DNA damage using ethyl acetate fractions from the cone of Pinus rigida × taeda (ERT). The antioxidant activity was evaluated using the DPPH, ABTS radical scavenging assay, reducing power assay, and Fe2+ chelating assay. Also, the contents of phenolic compounds and vitamin C related to antioxidant activity were analyzed to confirm phytochemicals. The DNA protective effect against oxidative stress was confirmed by the φX-174 RF I plasmid DNA cleavage assay. As a result, ERT showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. The results of reducing power and Fe2+ chelating activities were 77.32 ± 2.28% and 64.09 ± 1.01% at 200 ㎍/㎖. Also, ERT showed a DNA protective effect against oxidative stress.
Keywords
Cone of Pinus rigida ${\times}$ taeda; Antioxidant; DPPH; ABTS; plasmid DNA; ${\varphi}X-174$ RF I plasmid DNA;
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