• Journal of Microbiology and Biotechnology
• /
• v.13 no.3
• /
• pp.323-331
• /
• 2003

Bacteria and fungi are fundamental biotic components of natural biogeochemical cycles for metals and metalloids, and play important roles in dissolution, precipitation, oxidation and reduction processes. Some processes catalyzed by microorganisms also have important applications in environmental biotechnology in the areas of ore leaching and bioremediation.

• Bulletin of the Korean Chemical Society
• /
• v.23 no.12
• /
• pp.1806-1810
• /
• 2002

We have described the synthesis and biological activity of novel retinamide . The retinamide derivatives were synthesized by introducing functional side chains into the 4-hydroxy group of 4-HPR. The activities could be dependent on the side chain length, functional group, and hetero atom. The antiprolife-rative potential of the derivatives was assessed by MTT assay in HCT116 cancer cell lines.

• Journal of the Korean Applied Science and Technology
• /
• v.36 no.1
• /
• pp.208-216
• /
• 2019

The purpose of this study was to investigated the biological activities such as cytotoxic, anti-oxidant and anti-inflammatory using Mentha arvensis L. extracts. Mentha arvensis L. was prepared by extracting with DW and 80% ethanol, after cell viability was assessed by MTT assay using RAW 264.7 cells. Antioxidant activities, and Anti-inflammatory activities measured through changes in the levels of reactive oxygen species (ROS), nitric oxide (NO), leukotrien B4 (LTB4), and anti- or in-flammatory cytokines($IL-1{\beta}$, IL-6, tumor necrosis factor $(TNF)-{\alpha}$, and IL-10) on LPS-induced RAW 264.7 cells. All test measured by ELISA reader and Luminex. Mentha arvensis L. was no cytotoxic in water and 80% ethanol extracts, and Concentration of 100 ug/ml of 80% ethanol extract was suppressed the productions of ROS in LPS-induced RAW 264.7 cells. Also, Productions of NO, LTB4, inflammatory or anti-inflammatory cytokines showed efficacy change that dose-independent of all extracts. In other words, Mentha arvensis L. showed significant biological activities showing anti-inflammatory, and anti-oxidant. These results may be developed as a raw material for new health food and therapeutics to ease the symptoms mentioned above.

• Korean Journal of Microbiology
• /
• v.45 no.3
• /
• pp.239-245
• /
• 2009

Translation initiation factor eIF1A performs essential functions in various initiation steps including 43S preinitiation complex formation in eukaryotes, and contains a highly conserved oligonucleotide-binding (OB) fold. In our previous study, we discovered that eIF1A possesses RNA annealing activity and forms a stable complex with double-stranded RNA. In this study, we initiated site-directed mutations in eIF1A to find the active sites for these biochemical activities and to investigate whether they are essential functions for yeast cell growth. A truncated protein, eIF1A($\Delta$T), devoid of both N- and C-terminal domains but containing an intact OB-fold exhibited RNA annealing activity. In contrast, all point mutations in OB-fold domain, except R57D, impaired both RNA annealing and dsRNA binding activities, indicating that the intact OB-fold domain is required for both activities. Viabilities of the mutant yeast cells were not correlated with RNA annealing activity but with the in vivo protein stabilities in the case of R57D and K94D.

• Journal of Applied Biological Chemistry
• /
• v.44 no.4
• /
• pp.185-189
• /
• 2001

Essential oils of 21 herb plant samples, using spectrophotometric and paper disc agar diffusion methods under anaerobic conditions, were tested in vitro for their growth-inhibiting activities against Bifidobacterium bifidum, B. longum, Lactobacillus casei, Clostridium perfringens, and Escherichia coli. The responses varied with bacterial strains and plant oils. At 10 mg/disk, all essential oils did not inhibit beneficial intestinal bacteria, except for the oil of Alpinia officinarum and Melaleuca alternifolia against L. casei. Due to their strong growth-inhibitory activities against C. perfringens, E. coli, and L. casei, the activites of nine oils were evaluated at low concentrations. In test with C. perfringens at 1 mg/disk, the oils of Amyris balsamifera, Curcuma longa, M. alternifolia, and Trachyspermum ammi showed moderate activities. Moderate activities against E. coli were observed with the oils of M. alternifolia and T. ammi. These results may be indications of at least one of the pharmacological actions of the four herb plants.

• Journal of Korean Elementary Science Education
• /
• v.26 no.4
• /
• pp.468-474
• /
• 2007

The purpose of this study was to examine the elementary pre-service teachers' interest in biological topics and activities. The instrument to survey the interest of them consists of 8 domains containing 27 topic items and 7 domains containing 12 activity items(three-point from 0 to 2 Likert scale). Data were collected from 108 pre-service teachers. The interest level in the topics was high, but the interest level in the activities was average. There was not different between interest levels of females and males in both topic and activity components (p > .05). Among the topics, the 'genetics' domain correlated strongly with the domains of 'nutrition and digestion', 'circulation', 'respiration' and 'nervous system', and the 'respiration' domain correlated with the 'circulation' domain(r >.450, p<.01). The correlation between the domains of 'out-of class experience activity' and 'visual information activity', and 'experimental performance following procedure' and 'discussing and debating'(r >.450, p<.01). This results suggested that the educational contents including learning materials and experiential activities be developed to activate the interest of elementary pre-service teachers in biology.

• Journal of Korean Biological Nursing Science
• /
• v.11 no.2
• /
• pp.149-156
• /
• 2009

Purpose: This study was designed to identify the correlation among perceived health, depression and activities of daily living in low-income community dwelling elderly. Methods: The subjects consisted of 254 basic livelihood security person aged over 65, who live in house located in Buk-gu, Daegu city. The data was collected through interview with questionnaire from July 1 to August 20, 2009. Results: The average number of the perceived status in study subjects was 2.37. The average scores of activities of daily living (ADL) were 5.55. There was a significant relationship between perceived health status & ADL (r=.270, p=.01) and between depression & ADL (r=-.163, p=.01). The general characteristics which significantly affected depression was sex (t=-1.986, p=.49), education (F=8.968, p=.000), marital status (F=2.588, p=.037), occupation (t=33.258, p=.000). The general characteristics which significantly affected ADL was occupation (t=-7.677, p=.000). Conclusion: The finding of this study give useful information for constructing an intervention program and care for low-income elderly.

• Journal of Applied Biological Chemistry
• /
• v.53 no.3
• /
• pp.170-173
• /
• 2010

Antioxidant and anticoagulant activities of water extract and 70% ethanol extract of Phyllostachys pubescence leaf (PPL) produced in Geoje were investigated. Total polyphenol contents of PPL-water extract and PPL-70%, EtOH extract were measured as $80.6{\pm}3.2$ and $71.3{\pm}2.7$ mg/g, respectively. DPPH radical scavenging activity of PPL-water extract and PPL-70% EtOH extract were 50% and 90%, respectively. The anticoagulant activity, in intrinsic pathway of activated partial thromboplastin time (APTT) showed a concentration-dependency. This results showed that PPL-water extract and PPL-70% EtOH extract have high antioxidant and anticoagulant activities.

• Journal of Applied Biological Chemistry
• /
• v.60 no.1
• /
• pp.41-47
• /
• 2017

Genetic differentiation and antioxidant activities in ethanolic extracts from leaves of Bouea macrophylla Griffith were determined. The result revealed genetic differentiation among sour ma-praang, ma-yong and sweet ma-praang of B. macrophylla Griffith (${\phi}_{PT}=0.772$, p-value=0.000). In addition, high genetic diversities were found in sour ma-praang and sweet ma-praang populations (P: 51.4 and 57.1 %; He: 0.1900.035 and 0.2240.036, respectively), but low genetic diversity was found in ma-yong population (P: 8.6 %; He: 0.0350.021). Total phenolic contents of sour ma-praang, ma-yong and sweet ma-praang were estimated as $680.51{\pm}89.81$, $701.03{\pm}59.89$ and $530.85{\pm}41.23mg$ gallic acid/g extract, respectively. Free radical scavenging activities of sour mapraang, ma-yong and sweet ma-praang were found (1/EC50=4.17, 1.43 and 1.37, respectively) corresponding with metalchelating activities (1/EC50=0.83, 0.65 and 0.17, respectively). Therefore, the obtained data may be applied to cultivation and utilization of their leaves as source of natural phenolics and antioxidants.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.4
• /
• pp.289-294
• /
• 2004

This study was carried out to investigate the antioxidative enzymes and isozymes between chilling-tolerant and -susceptible varieties at the booting stage under cold water stress $(13^{\circ}C)$ in japonica rice. Total SOD, CAT, POX, and GR activities on the basis of protein were found to be important factors to defend cold water stress. Especially, SOD and CAT activities showed distinctive differences between chilling-tolerant and -susceptible varieties. Chilling-tolerant varieties were higher than chilling-susceptible varieties for SOD and CAT activities. One of eight isozyme bands for SOD was a inducible isoform. Three isozymes for CAT and one isozyme for POX were closely correlated with defense to cold water stress. Total GR activities except Stejaree 45 on the basis fresh weight and POX were increased by cold water stress, but there was no difference between chilling-tolerant and -sus­ceptible varieties.