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Activity and Isozyme Profile of Antioxidative Enzymes at Booting Stage of Rice Treated with Cold Water  

Kim Ki-Young (Honam Agricultural Research Institute, NICS, RDA)
Kim Bo-Kyeong (Honam Agricultural Research Institute, NICS, RDA)
Shin Mun-Sik (Honam Agricultural Research Institute, NICS, RDA)
Choung Jin-Il (Honam Agricultural Research Institute, NICS, RDA)
Ko Jae-Kweon (Honam Agricultural Research Institute, NICS, RDA)
Kim Jung-Kon (Honam Agricultural Research Institute, NICS, RDA)
Lim Jung-Hyun (Faculty of biological Resources, and Institute for Agricultural Science and Technology, Chonbuk National University)
Yun Song-Joon (Faculty of biological Resources, and Institute for Agricultural Science and Technology, Chonbuk National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.49, no.4, 2004 , pp. 289-294 More about this Journal
Abstract
This study was carried out to investigate the antioxidative enzymes and isozymes between chilling-tolerant and -susceptible varieties at the booting stage under cold water stress $(13^{\circ}C)$ in japonica rice. Total SOD, CAT, POX, and GR activities on the basis of protein were found to be important factors to defend cold water stress. Especially, SOD and CAT activities showed distinctive differences between chilling-tolerant and -susceptible varieties. Chilling-tolerant varieties were higher than chilling-susceptible varieties for SOD and CAT activities. One of eight isozyme bands for SOD was a inducible isoform. Three isozymes for CAT and one isozyme for POX were closely correlated with defense to cold water stress. Total GR activities except Stejaree 45 on the basis fresh weight and POX were increased by cold water stress, but there was no difference between chilling-tolerant and -sus­ceptible varieties.
Keywords
rice; leaf; antioxidative enzymes; isozymes; booting stage; cold water stress;
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