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Isolation and Identification of Postharvest Spoilage Fungi from Mulberry Fruit in Korea

  • Kwon, O-Chul;Ju, Wan-Taek;Kim, Hyun-Bok;Sung, Gyoo-Byung;Kim, Yong-Soon
    • Korean Journal of Environmental Agriculture
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    • v.37 no.3
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    • pp.221-228
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    • 2018
  • BACKGROUND: Spoilage fungi can reduce the shelf life of fresh fruits and cause economic losses by lowering quality. Especially, mulberry fruits have high sensitivity to fungal attack due to their high water content (> 70%) and soft texture. In addition, the surface of these fruits is prone to damage during harvesting and postharvest handling. However, any study on postharvest spoilage fungi in mulberry fruit has not been reported in Korea. This study aimed to examine the spoilage fungi occurring in mulberry fruits during storage after harvest. METHODS AND RESULTS: In this study, we isolated postharvest spoilage fungi from mulberry fruits stored in refrigerator (fresh fruits) and deep-freezer (frozen fruits) and identified them. In the phylogenetic analysis based on comparisons of the ITS rDNA sequences, the 18 spoilage fungi isolated from mulberry fruits and the 25 reference sequences were largely divided into seven groups that were subsequently verified by high bootstrap analysis of 73 to 100. Alternaria spp. including A. alternate and A. tenuissima, were the most frequently isolated fungi among the spoilage isolates: its occurrence was the highest among the 18 isolates (38.9%). CONCLUSION: The findings of this study will be helpful for increasing the shelf life of mulberry fruits through the application of appropriate control measures against infection by spoilage fungi during storage.

Secure 6LoWPAN Neighbor Discovery Address Registration Protocol (안전한 6LoWPAN Neighbor Discovery 주소 등록 프로토콜)

  • Han, Sang-woo;Park, Chang-seop
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.29 no.1
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    • pp.17-28
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    • 2019
  • 6LoWPAN based on IEEE 802.15.4 is a realistic standard platform for various Internet of Things (IoT) applications. To bootstrap the LoWPAN (Low-power Wireless Personal Area Network), each device must perform 6LoWPAN-ND address registration to assign a unique IPv6 address. Without adequate security mechanisms, 6LoWPAN-ND is vulnerable to a variety of security attacks including corrupted node attacks. Several security mechanisms have been proposed as a supplement to the vulnerability, but the vulnerability exists because it relies solely on IEEE 802.15.4 hop-by-hop security. In this paper, we propose and analyze a vulnerability of 6LoWPAN-ND address registration and a new security mechanism suitable for preventing the attack of damaged node. It also shows that the proposed security mechanism is compatible with the Internet Engineering Task Force (IETF) standard and is more efficient than the mechanism proposed in the IETF 6 lo WG.

Morphological and Molecular Identification of Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae) in Thailand

  • Wongsawad, Chalobol;Nantarat, Nattawadee;Wongsawad, Pheravut;Butboonchoo, Preeyaporn;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.57 no.3
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    • pp.257-264
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    • 2019
  • We tried a series of morphological and molecular approaches to identify a new species of Stellantchasmus (Digenea: Heterophyidae) originating from the wrestling half-beaked fish, Dermogenys pusillus of Thailand. Adult worm samples of the new species were recovered from hamsters experimentally infected with the metacercariae from D. pusillus in Thailand. Two isolates (Thai and Korean) of Stellantchasmus falcatus were used as comparative control groups. Worm samples of 3 Stellantchasmus groups were morphologically observed and molecularly analyzed with the mitochondrial cytochrome c oxidase 1 gene. The morphological characteristics of S. dermogenysi n. sp. are similar to S. falcatus originating from brackish water fish, but minor difference was noted including the absence of the prepharynx, position of the ovary near the ceca end, smaller body size, and shorter esophageal length. A phylogenetic tree derived from neighbor-joining and maximum-likelihood methods suggests that S. dermogenysi n. sp. is separated from S. falcatus supported by high bootstrap values. The relative divergences persist between these host-specific trematodes, which we suggest should be recognized as 2 distinct species. Comparisons of S. dermogenysi n. sp. with S. falcatus isolated from mullets in Thailand and Korea indicate a genetic divergence of mitochondrial DNA of 19.4% and 21.7%, respectively. By the present study, a new species, Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae), is proposed in Thailand based on molecular evidences, in addition to minor morphological differences between S. falcatus and the new species.

The Mediating Effect of the Organizational Commitment between the Coworker Relations and the Burnout of the Professionals in Community Rehabilitation Facilities (장애인지역사회재활시설 종사자의 동료관계와 소진의 관계에서 조직헌신의 매개효과)

  • Lee, Byoung-Rock
    • Journal of the Korea Convergence Society
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    • v.12 no.6
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    • pp.217-223
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    • 2021
  • The research purpose is analyzing the relationship between the coworker relations and the burnout of professionals in community rehabilitation facilities for the disabled. And this study verifies the mediating effect of organizational commitment between the two variables. We conducted a survey of professionals working in 14 facilities of Daejeon and Chungnam area, and the 352 data were utilized for analysis. The analysis results are as follows. 1) The coworker relations of professionals influenced the burnout negatively and the organizational commitment positively. 2) In addition, the mediating effect of organizational commitment was testified between the coworker relations and the burnout. Based on these research findings, several measures were suggested to reduce the professionals' burnout.

Novel nomogram-based integrated gonadotropin therapy individualization in in vitro fertilization/intracytoplasmic sperm injection: A modeling approach

  • Ebid, Abdel Hameed IM;Motaleb, Sara M Abdel;Mostafa, Mahmoud I;Soliman, Mahmoud MA
    • Clinical and Experimental Reproductive Medicine
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    • v.48 no.2
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    • pp.163-173
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    • 2021
  • Objective: This study aimed to characterize a validated model for predicting oocyte retrieval in controlled ovarian stimulation (COS) and to construct model-based nomograms for assistance in clinical decision-making regarding the gonadotropin protocol and dose. Methods: This observational, retrospective, cohort study included 636 women with primary unexplained infertility and a normal menstrual cycle who were attempting assisted reproductive therapy for the first time. The enrolled women were split into an index group (n=497) for model building and a validation group (n=139). The primary outcome was absolute oocyte count. The dose-response relationship was tested using modified Poisson, negative binomial, hybrid Poisson-Emax, and linear models. The validation group was similarly analyzed, and its results were compared to that of the index group. Results: The Poisson model with the log-link function demonstrated superior predictive performance and precision (Akaike information criterion, 2,704; λ=8.27; relative standard error (λ)=2.02%). The covariate analysis included women's age (p<0.001), antral follicle count (p<0.001), basal follicle-stimulating hormone level (p<0.001), gonadotropin dose (p=0.042), and protocol type (p=0.002 and p<0.001 for short and antagonist protocols, respectively). The estimates from 500 bootstrap samples were close to those of the original model. The validation group showed model assessment metrics comparable to the index model. Based on the fitted model, a static nomogram was built to improve visualization. In addition, a dynamic electronic tool was created for convenience of use. Conclusion: Based on our validated model, nomograms were constructed to help clinicians individualize the stimulation protocol and gonadotropin doses in COS cycles.

The effect of information source and content informativeness on acceptance intention in a live commerce shopping environment - The mediating effects of trust - (라이브 커머스 쇼핑환경에서 정보원 특성과 콘텐츠 정보성이 소비자 수용의도에 미치는 효과 - 신뢰의 매개효과를 중심으로 -)

  • Choi, Mi Young
    • The Research Journal of the Costume Culture
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    • v.29 no.4
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    • pp.554-571
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    • 2021
  • The purpose of this study is to investigate the mediating effect of trust when a media broadcaster (such as a disc jockey [DJ]) acting as an information source and the content they provide during live commerce streaming affect acceptance intention. Live commerce is increasing rapidly, offering a new fashion distribution channel by supplementing possible shortcomings of existing online shopping. Data was collected for the empirical study from female consumers in their 20s who actively accepted fashion technology. Statistical analysis of the data was conducted using IBM SPSS Macro Process 3.5. First, the reliability and validity of the variables for information source characteristics, content informativeness, trust, and acceptance intention were verified, and each variable was confirmed as a single factor. Bootstrap analysis was performed using Macro Process Model 4 to reveal the effects of information source characteristics and content informativeness on acceptance intention. As a result of analyzing the mediating effect for each path model with trust as a parameter, it was found that both the direct and indirect effects of the mediating path were significant. This result means that the characteristics of information sources and content informativeness are partially mediated by trust. Therefore, to promote consumer behavior in a live commerce shopping environment, it is necessary to enhance trust. This can be achieved by a media broadcaster with fashion expertise to increase the perception of the attractiveness of the information source and to improve the usefulness of the fashion information being delivered.

Effects of Second Victim Experiences after Patient Safety Incidents on Nursing Practice Changes in Korean Clinical Nurses: The Mediating Effects of Coping Behaviors (환자안전사건과 관련된 임상간호사의 이차피해경험이 간호실무변화에 미치는 영향: 대처의 매개효과)

  • Jeong, Seohee;Jeong, Seok Hee
    • Journal of Korean Academy of Nursing
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    • v.51 no.4
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    • pp.489-504
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    • 2021
  • Purpose: This study was investigated the mediating effect of coping behaviors in the relationship between the second victim experiences after patient safety incidents and the nursing practice changes. Methods: A cross-sectional survey was performed using structured questionnaires. Participants were 218 clinical nurses in general tertiary hospitals in South Korea. Data were collected through an online survey and snowball sampling from August 11 to September 6 2020. Data were analyzed using SPSS 23.0 program. A mediation analysis was performed using multiple regression and a simple mediation model applying the PROCESS macro with 95% bias-corrected bootstrap confidence interval. Results: The mean scores of second victim experiences was 3.41/5. Approach coping (β = .55, p < .001) and the avoidant coping (β = - .23, p = .001) showed mediation effects in the relationship between second victim experiences and constructive change in nursing practice. Avoidant coping (β = .29, p < .001) showed a mediation effect in the relationship between second victim experiences and defensive change in nursing practice. Conclusion: Coping behaviors has a mediating effect on the relationship between second victim experiences and nursing practice changes. To ensure that nurses do not experience second victim, medical institutions should have a culture of patient safety that employs a systematic approach rather than blame individuals. They also need to develop strategies that enhance approach coping and reducing avoidant coping to induce nurses' constructive practice changes in clinical nurses in experiencing second victims due to patient safety incidents.

Mediating Effect of Job Satisfaction between Directors' Leadership and Workers' Organizational Commitment in Senior Clubs (시니어클럽 관장의 리더십과 종사자의 조직헌신의 관계에서 직무만족의 매개효과)

  • Lee, Byoung-Rock
    • Journal of Convergence for Information Technology
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    • v.12 no.4
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    • pp.199-204
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    • 2022
  • This study analyzed mediating effect of job satisfaction between directors' leadership and workers' organizational commitment in senior clubs. A survey of 288 workers in Chungcheong and Daejeon was conducted. The statistical analysis methods were frequency, correlation, confirmatory factor analysis, and bootstrap. The analysis result was that directors' leadership had positive effect on workers' organizational commitment and mediating effect of job satisfaction was verified. For improving directors' leadership, it is necessary to develop various leadership education and training programs, to induce voluntary participation in the programs, and to include the participation of leadership program in facility evaluation. To advance workers' job satisfaction, we need various exchange programs with colleagues and peer groups, mentoring and rest service to manage emotional labor, and professional job education and supervision.

Complete mitochondrial genome of Rotunda rotundapex Miyata & Kishida 1990 (Lepidoptera: Bombycidae), which was named as Bombyx shini Park & Sohn 2002

  • Park, Jeong Sun;Kim, Min Jee;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.44 no.2
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    • pp.55-64
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    • 2022
  • Bombyx shini Park & Sohn, 2002 (Lepidoptera: Bombycidae), which was listed as an endemic species in South Korea has recently been renamed as the East Asian silk moth Rotunda rotundapex Miyata & Kishida, 1990 (Lepidoptera: Bombycidae). In this study, we sequenced the complete mitochondrial genome (mitogenome) of the R. rotundapex to announce genomic characteristics and to clarify its validity with a new name. The 15,294-bp long complete mitogenome comprises a typical set of genes [13 protein-coding genes (PCGs), 2 rRNA genes, and 22 tRNA genes] and one major noncoding, A + T-rich region, with an arrangement identical to that observed in most lepidopteran mitogenomes. The A/T content of the whole mitogenome was 79.22%; however, it varied among the regions/genes as follows: A + T-rich region, 91.62%; srRNA, 84.67%; lrRNA, 83.01%; tRNAs, 81.43%; and PCGs, 77.46%. Phylogenetic analyses of 35 species in the Bombycoidea superfamily showed the sister relationship between the families Sphingidae and Bombycidae s. str., with the higher nodal support [bootstrap support (BS) = 78%]. The Saturniidae was placed as the sister to the two families, but the nodal support for this relationship was low (BS = 53%). Current R. rotundapex was placed together with previously reported con-species with the highest nodal support, forming a separate clade from Bombyx, validating that B. shini can have a new genus name, Rotunda. However, the Korean R. rotundapex showed a substantial sequence divergence at 5.28% to that originated from an individual of type locality Taiwan in 1,459-bp of COI sequences. Considering such a high sequence divergence an additional study, which includes morphological and DNA barcoding data from further extensive distributional range maybe is needed for further robust taxonomic conclusion.

The Moderating Effect of Organizational Justice on the Relationship between Self-Efficacy and Nursing Performance in Clinical Nurses (임상간호사의 자기효능감과 간호업무성과의 관계에서 조직공정성의 조절효과)

  • Kim, Ju-Ra;Ko, Yukyung;Lee, Youngjin;Kim, Chun-Ja
    • Journal of Korean Academy of Nursing
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    • v.52 no.5
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    • pp.511-521
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    • 2022
  • Purpose: This study aimed to examine the moderating effect of organizational justice on the relationship between self-efficacy and nursing performance among clinical nurses. Methods: In January 2021, a cross-sectional survey was conducted with 224 clinical nurses recruited from a university-affiliated hospital in Suwon, South Korea. Participants completed online-based, self-report structured questionnaires. Collected data were analyzed using multiple regression and a simple model of PROCESS macro with a 95% bias-corrected bootstrap confidence interval. Results: Self-efficacy and organizational justice were found to be significant predictors of nursing performance. These two predictors explained the additional 34.8% variance of nursing performance in the hierarchical regression model, after adjusting the other covariates. In addition, organizational justice moderated the relationship between self-efficacy and nursing performance among the clinical nurses. In particular, at low self-efficacy level, participants with high organizational justice had higher nursing performance compared to those with low organizational justice. Conclusion: Enhancing organizational justice can be used as an organizational strategy for improving the organizational culture in terms of distribution, procedure, and interaction. Ultimately, these efforts will contribute to the improvement of nursing performance through a synergistic effect on organizational justice beyond nurses' individual competency and self-efficacy.