• 제목/요약/키워드: Active Cell

검색결과 2,471건 처리시간 0.026초

A Laboratory-Scale Study of the Applicability of a Halophilic Sediment Bioelectrochemical System for in situ Reclamation of Water and Sediment in Brackish Aquaculture Ponds: Establishment, Bacterial Community and Performance Evaluation

  • Pham, Hai The;Tran, Hien Thi;Vu, Linh Thuy;Dang, Hien The;Nguyen, Thuy Thu Thi;Dang, Thu Ha Thi;Nguyen, Mai Thanh Thi;Nguyen, Huy Quang;Kim, Byung Hong
    • Journal of Microbiology and Biotechnology
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    • 제29권7호
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    • pp.1104-1116
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    • 2019
  • In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. This test tank and the control (not containing the SBES) were fed with shrimp feed in a scheme that mimics a situation where 50% of feed is uneaten. After the SBES was inoculated with microbial sources from actual shrimp pond sediments, electricity generation was well observed from the first experimental week, indicating successful enrichment of electrochemically active bacteria in the test tank sediment. The electricity generation became steady after 3 weeks of operation, with an average current density of $2.3mA/m^2$ anode surface and an average power density of $0.05mW/m^2$ anode surface. The SBES removed 20-30% more COD of the tank water, compared to the control. After 1 year, the SBES also reduced the amount of sediment in the tank by 40% and thus could remove approximately 40% more COD and approximately 52% more nitrogen from the sediment, compared to the control. Insignificant amounts of nitrite and nitrate were detected, suggesting complete removal of nitrogen by the system. PCR-DGGE-based analyses revealed the dominant presence of Methylophilus rhizosphaerae, Desulfatitalea tepidiphila and Thiothrix eikelboomii, which have not been found in bioelectrochemical systems before, in the bacterial community in the sediment of the SBES-containing tank. The results of this research demonstrate the potential application of SBESs in helping to reduce water pollution threats, fish and shrimp disease risks, and thus farmers' losses.

Effects of Olanzapine on the Pacemaker Potentials of Interstitial Cells of Cajal in Murine Small Intestine (생쥐 소장 카할세포에서 발생하는 향도잡이 기능의 올란자핀 효능에 관한 연구)

  • Kim, Byung Joo
    • Journal of Life Science
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    • 제29권9호
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    • pp.1010-1015
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    • 2019
  • The interstitial cells of Cajal (ICCs) are the pacemaker cells in the gastrointestinal (GI) tract. In the present study, the effects of olanzapine, an atypical antipsychotic agent, on pacemaker potentials in cultured ICCs from the small intestine of the mouse were investigated. The whole-cell patch-clamp configuration was used to record pacemaker potentials from cultured ICCs. Olanzapine produced pacemaker depolarizations in a concentration-dependent manner in current clamp mode. Methoctramine, a muscarinic $M_2$ receptor antagonist, did not inhibit olanzapine-induced pacemaker depolarizations, whereas 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) muscarinic $M_3$ receptor antagonist did inhibit it. When guanosine 5'-[${\beta}$-thio] diphosphate (GDP-${\beta}$-S; 1 mM) was in the pipette solution, olanzapine-induced pacemaker depolarization was blocked. Also, low $Na^+$ solution externally eliminated the generation of pacemaker potentials and inhibited the olanzapine-induced pacemaker depolarizations. Additionally, the nonselective cation channel blocker, flufenamic acid, inhibited the olanzapine-induced pacemaker depolarizations. Pretreatment with U-73122, an active phospholipase C (PLC) inhibitor, also eliminated the generation of pacemaker potentials and suppressed the olanzapine-induced pacemaker depolarizations. These results suggested that olanzapine modulates the pacemaker potentials through muscarinic $M_3$ receptor activation by G protein-dependent external $Na^+$ and PLC pathway in the ICCs. Therefore, olanzapine could affect intestinal motility through ICCs.

Current Perspectives on the Effects of Plant Growth-promoting Rhizobacteria (식물생장촉진 근권미생물의 영향에 대한 연구 현황 및 전망)

  • Le, Thien Tu Huynh;Jun, Sang Eun;Kim, Gyung-Tae
    • Journal of Life Science
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    • 제29권11호
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    • pp.1281-1293
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    • 2019
  • The rhizosphere is the active zone where plant roots communicate with the soil microbiome, each responding to the other's signals. The soil microbiome within the rhizosphere that is beneficial to plant growth and productivity is known as plant growth-promoting rhizobacteria (PGPR). PGPR take part in many pivotal plant processes, including plant growth, development, immunity, and productivity, by influencing acquisition and utilization of nutrient molecules, regulation of phytohormone biosynthesis, signaling, and response, and resistance to biotic- and abiotic-stresses. PGPR also produce secondary compounds and volatile organic compounds (VOCs) that elicit plant growth. Moreover, plant roots exude attractants that cause PGPR to aggregate in the rhizosphere zone for colonization, improving soil properties and protecting plants against pathogenic factors. The interactions between PGPR and plant roots in rhizosphere are essential and interdependent. Many studies have reported that PGPR function in multiple ways under the same or diverse conditions, directly and indirectly. This review focuses on the roles and strategies of PGPR in enhancing nutrient acquisition by nutrient fixation/solubilization/mineralization, inducing plant growth regulators/phytohormones, and promoting growth and development of root and shoot by affecting cell division, elongation, and differentiation. We also summarize the current knowledge of the effects of PGPR and the soil microbiota on plants.

Anti-obesity Effect of the Flavonoid Rich Fraction from Mulberry Leaf Extract (뽕잎 추출물 기원 Flavonoid Rich Fraction의 항비만효과)

  • Go, Eun Ji;Ryu, Byung Ryeol;Yang, Su Jin;Baek, Jong Suep;Ryu, Su Ji;Kim, Hyun Bok;Lim, Jung Dae
    • Korean Journal of Medicinal Crop Science
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    • 제28권6호
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    • pp.395-411
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    • 2020
  • Background: This study investigated the anti-obesity effect of the flavonoid rich fraction (FRF) and its constituent, rutin obtained from the leaf of Morus alba L., on the lipid accumulation mechanism in 3T3-L1 adipocyte and C57BL/6 mouse models. Methods and Results: In Oil Red O staining, FRF (1,000 ㎍/㎖) treatments showed inhibition rate of 35.39% in lipid accumulation compared to that in the control. AdipoRedTM assay indicated that the triglyceride content in 3T3-L1 adipocytes treated with FRF (1,000 ㎍/㎖) was reduced to 23.22%, and free glycerol content was increased to 106.04% that of the control. FRF and its major constituent, rutin affected mRNA gene expression. Rutin contributed to the inhibition of Sterol regulatory element binding protein-1c (SREBP-1c) gene expression, and inhibited the transcription factors SREBP-1c, peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). In addition, the effect of FRF administration on obesity development in C57BL/6 mice fed high-fat diet (HFD) was investigated. FRF suppressed weight gain, and reduced liver triglyceride and leptin secretion. FRF exerted potential anti-inflammatory effects by improving insulin resistance and adiponectin levels, and could thus be used to help counteract obesity. The mRNA expressions of PPAR-γ, FAS, ACC, and CPT-1 were determined in liver tissue. Quantitative real-time PCR analysis was also performed to evaluate the expression of IL-1β, IL-6, and TNF-α in epididymal adipose tissue. Compared to the control group, mice fed the HFD showed the up-regulation in PPAR-γ, FAS, IL-6, and TNF-α genes, and down-regulation in CPT1 gene expression. FRF treatement markedly reduced the expression of PPAR-γ, FAS, IL-6, and TNF-α compared to those in HFD control, whereas increased the expression level of CPT1. Conclusions: These results suggest that the FRF and its major active constituent, rutin, can be used as effective anti-obesity agents.

Topic Modeling-Based Domestic and Foreign Public Data Research Trends Comparative Analysis (토픽 모델링 기반의 국내외 공공데이터 연구 동향 비교 분석)

  • Park, Dae-Yeong;Kim, Deok-Hyeon;Kim, Keun-Wook
    • Journal of Digital Convergence
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    • 제19권2호
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    • pp.1-12
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    • 2021
  • With the recent 4th Industrial Revolution, the growth and value of big data are continuously increasing, and the government is also actively making efforts to open and utilize public data. However, the situation still does not reach the level of demand for public data use by citizens, At this point, it is necessary to identify research trends in the public data field and seek directions for development. In this study, in order to understand the research trends related to public data, the analysis was performed using topic modeling, which is mainly used in text mining techniques. To this end, we collected papers containing keywords of 'Public data' among domestic and foreign research papers (1,437 domestically, 9,607 overseas) and performed topic modeling based on the LDA algorithm, and compared domestic and foreign public data research trends. After analysis, policy implications were presented. Looking at the time series by topic, research in the fields of 'personal information protection', 'public data management', and 'urban environment' has increased in Korea. Overseas, it was confirmed that research in the fields of 'urban policy', 'cell biology', 'deep learning', and 'cloud·security' is active.

Far-infrared rays enhance mitochondrial biogenesis and GLUT3 expression under low glucose conditions in rat skeletal muscle cells

  • Seo, Yelim;Kim, Young-Won;Lee, Donghee;Kim, Donghyeon;Kim, Kyoungseo;Kim, Taewoo;Baek, Changyeob;Lee, Yerim;Lee, Junhyeok;Lee, Hosung;Jang, Geonwoo;Jeong, Wonyeong;Choi, Junho;Hwang, Doegeun;Suh, Jung Soo;Kim, Sun-Woo;Kim, Hyoung Kyu;Han, Jin;Bang, Hyoweon;Kim, Jung-Ha;Zhou, Tong;Ko, Jae-Hong
    • The Korean Journal of Physiology and Pharmacology
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    • 제25권2호
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    • pp.167-175
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    • 2021
  • Far-infrared rays (FIR) are known to have various effects on atoms and molecular structures within cells owing to their radiation and vibration frequencies. The present study examined the effects of FIR on gene expression related to glucose transport through microarray analysis in rat skeletal muscle cells, as well as on mitochondrial biogenesis, at high and low glucose conditions. FIR were emitted from a bio-active material coated fabric (BMCF). L6 cells were treated with 30% BMCF for 24 h in medium containing 25 or 5.5 mM glucose, and changes in the expression of glucose transporter genes were determined. The expression of GLUT3 (Slc2a3) increased 2.0-fold (p < 0.05) under 5.5 mM glucose and 30% BMCF. In addition, mitochondrial oxygen consumption and membrane potential (ΔΨm) increased 1.5- and 3.4-fold (p < 0.05 and p < 0.001), respectively, but no significant change in expression of Pgc-1a, a regulator of mitochondrial biogenesis, was observed in 24 h. To analyze the relationship between GLUT3 expression and mitochondrial biogenesis under FIR, GLUT3 was down-modulated by siRNA for 72 h. As a result, the ΔΨm of the GLUT3 siRNA-treated cells increased 3.0-fold (p < 0.001), whereas that of the control group increased 4.6-fold (p < 0.001). Moreover, Pgc-1a expression increased upon 30% BMCF treatment for 72 h; an effect that was more pronounced in the presence of GLUT3. These results suggest that FIR may hold therapeutic potential for improving glucose metabolism and mitochondrial function in metabolic diseases associated with insufficient glucose supply, such as type 2 diabetes.

2,7-Phloroglucinol-6,6-Bieckol Increases Glucose Uptake by Promoting GLUT4 Translocation to Plasma Membrane in 3T3-L1 Adipocytes (2,7-Phloroglucinol-6,6-Bieckol의 3T3-L1 지방세포에서 GLUT4 활성화를 통한 포도당 흡수 증진 효과)

  • Lee, Hyun-Ah;Han, Ji⁃Sook
    • Journal of Life Science
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    • 제31권8호
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    • pp.729-735
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    • 2021
  • Type 2 diabetes occurs when there is an abnormality in the tissue's ability to absorb glucose. Glucose uptake and metabolism by insulin are the basic mechanisms that maintain blood sugar. Glucose uptake goes through various signaling steps initiated by the binding of insulin to receptors on the cell surface. In line with the foregoing, the purpose of this study was to investigate the effect of 2,7-phloroglucinol-6,6-bieckol (PHB), an active compound isolated from Ecklonia cava, on glucose uptake in 3T3-L1 adipocytes. Notably, PHB increased glucose uptake in a dose-dependent manner owing to the enhanced glucose transporter type 4 (GLUT4) expression in the plasma membrane of 3T3-L1 adipocytes. These effects of PHB were attributed to the phosphorylation of insulin receptor substrate-1 and protein kinase B (PKB or AKT), as well as to the phosphoinositide 3-kinase (PI3K) activation in the insulin signaling pathway. PHB also stimulated 5' AMP-activated protein kinase (AMPK) phosphorylation and activation. The phosphorylation and activation of the PI3K/AKT and AMPK pathways by PHB were identified using wortmannin (a PI3K inhibitor) and compound C (an AMPK inhibitor). In this study, we showed that PHB can increase glucose uptake in 3T3-L1 adipocytes by promoting GLUT4 translocation to the plasma membrane via the PI3K and AMPK pathways. The results indicate that PHB may help improve insulin sensitivity.

Multi-Layered Sintered Porous Transport Layers in Alkaline Water Electrolysis (다층 소결메쉬 확산체를 이용한 알칼라인 수전해 셀)

  • YEOM, SANG HO;YUN, YOUNG HWA;CHOI, SEUNGWOOK;KWON, JIHEE;LEE, SECHAN;LEE, JAE HUN;LEE, CHANGSOO;KIM, MINJOONG;KIM, SANG-KYUNG;UM, SUKKEE;KIM, CHANG-HEE;CHO, WON CHUL;CHO, HYUN-SEOK
    • Journal of Hydrogen and New Energy
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    • 제32권6호
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    • pp.442-454
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    • 2021
  • The porous transport layer (PTL) is essential to effectively remove oxygen and hydrogen gas from the electrode surface at high current density operation conditions. In this study, the effect of PTL with different characteristics such as pore size, pore gradient, interfacial coating was investigated by multi-layered sintered mesh. A water electrolysis single cell of active area of the 34.56 cm2 was constructed, and IV performance and impedance analysis were conducted in the range of 0 to 2.0 A/cm2. It was confirmed that the multi-layered sintered mesh PTL, which have an average pore size of 25 to 57 ㎛ and a larger pore gradient, removed bubbles effectively and thus seemed to improve IV performance. Also, it was confirmed that the catalytic metals such as Ni, NiMo coating on the PTL reduced activation overpotential, but increased mass transport overpotential.

Combination Therapy of the Active KRAS-Targeting Antibody inRas37 and a PI3K Inhibitor in Pancreatic Cancer

  • Lee, Ji Eun;Woo, Min Gyu;Jung, Kyung Hee;Kang, Yeo Wool;Shin, Seung-Min;Son, Mi Kwon;Fang, Zhenghuan;Yan, Hong Hua;Park, Jung Hee;Yoon, Young-Chan;Kim, Yong-Sung;Hong, Soon-Sun
    • Biomolecules & Therapeutics
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    • 제30권3호
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    • pp.274-283
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    • 2022
  • KRAS activating mutations, which are present in more than 90% of pancreatic cancers, drive tumor dependency on the RAS/mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/AKT signaling pathways. Therefore, combined targeting of RAS/MAPK and PI3K/AKT signaling pathways may be required for optimal therapeutic effect in pancreatic cancer. However, the therapeutic efficacy of combined MAPK and PI3K/AKT signaling target inhibitors is unsatisfactory in pancreatic cancer treatment, because it is often accompanied by MAPK pathway reactivation by PI3K/AKT inhibitor. Therefore, we developed an inRas37 antibody, which directly targets the intra-cellularly activated GTP-bound form of oncogenic RAS mutation and investigated its synergistic effect in the presence of the PI3K inhibitor BEZ-235 in pancreatic cancer. In this study, inRas37 remarkably increased the drug response of BEZ-235 to pancreatic cancer cells by inhibiting MAPK reactivation. Moreover, the co-treatment synergistically inhibited cell proliferation, migration, and invasion and exhibited synergistic anticancer activity by inhibiting the MAPK and PI3K pathways. The combined administration of inRas37and BEZ-235 significantly inhibited tumor growth in mouse models. Our results demonstrated that inRas37 synergistically increased the antitumor activity of BEZ-235 by inhibiting MAPK reactivation, suggesting that inRas37 and BEZ-235 co-treatment could be a potential treatment approach for pancreatic cancer patients with KRAS mutations.

Nanofibers Comprising Mo2C/Mo2N Nanoparticles and Reduced Graphene Oxide as Functional Interlayers for Lithium-Sulfur Batteries (Mo2C/Mo2N 나노 입자와 환원된 그래핀 옥사이드가 복합된 나노 섬유 중간층이 적용된 리튬-황 전지)

  • Lee, Jae Seob;Yang, Ji Hoon;Cho, Jung Sang
    • Korean Chemical Engineering Research
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    • 제60권4호
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    • pp.574-581
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    • 2022
  • Nanofibers comprising reduced graphene oxide (rGO) and Mo2C/Mo2N nanoparticles (Mo2C/Mo2N rGO NFs) were prepared for a functional interlayer of Li-S batteries (LSBs). The well-dispersed Mo2C and Mo2N nanoparticles in the nanofiber structure served as active polar sites for efficient immobilization of dissolved lithium polysulfide. The rGO nanosheets in the structure also provide conductive channels for fast ion/electron transport during charging-discharging and ensured reuse of lithium polysulfide during redox reactions through a fast charge transfer process. As a result, the cell assembled with Mo2C/Mo2N rGO NFs-coated separator and pure sulfur electrode (70 wt% of sulfur content and 2.1 mg cm-2 of sulfur loading) showed a stable discharge capacity of 476 mA h g-1 after 400 charge-discharge cycles at 0.1 C. Furthermore, it exhibited a discharge capacity of 574 mA h g-1 even at a high current density of 1.0 C. Therefore, we believe that the proposed unique nanostructure synthesis strategy could provide new insights into the development of sustainable and highly conductive polar materials as functional interlayers for high performance LSBs.