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http://dx.doi.org/10.7783/KJMCS.2020.28.6.395

Anti-obesity Effect of the Flavonoid Rich Fraction from Mulberry Leaf Extract  

Go, Eun Ji (Department of Bio-Health Convergence, Kangwon National University)
Ryu, Byung Ryeol (Department of Bio-Health Convergence, Kangwon National University)
Yang, Su Jin (Department of Herbal Medicine Resource, Kangwon National University)
Baek, Jong Suep (Department of Bio-Health Convergence, Kangwon National University)
Ryu, Su Ji (Department of Bio-Health Convergence, Kangwon National University)
Kim, Hyun Bok (National Institute of Agricultural Sciences, RDA)
Lim, Jung Dae (Department of Bio-Health Convergence, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.28, no.6, 2020 , pp. 395-411 More about this Journal
Abstract
Background: This study investigated the anti-obesity effect of the flavonoid rich fraction (FRF) and its constituent, rutin obtained from the leaf of Morus alba L., on the lipid accumulation mechanism in 3T3-L1 adipocyte and C57BL/6 mouse models. Methods and Results: In Oil Red O staining, FRF (1,000 ㎍/㎖) treatments showed inhibition rate of 35.39% in lipid accumulation compared to that in the control. AdipoRedTM assay indicated that the triglyceride content in 3T3-L1 adipocytes treated with FRF (1,000 ㎍/㎖) was reduced to 23.22%, and free glycerol content was increased to 106.04% that of the control. FRF and its major constituent, rutin affected mRNA gene expression. Rutin contributed to the inhibition of Sterol regulatory element binding protein-1c (SREBP-1c) gene expression, and inhibited the transcription factors SREBP-1c, peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). In addition, the effect of FRF administration on obesity development in C57BL/6 mice fed high-fat diet (HFD) was investigated. FRF suppressed weight gain, and reduced liver triglyceride and leptin secretion. FRF exerted potential anti-inflammatory effects by improving insulin resistance and adiponectin levels, and could thus be used to help counteract obesity. The mRNA expressions of PPAR-γ, FAS, ACC, and CPT-1 were determined in liver tissue. Quantitative real-time PCR analysis was also performed to evaluate the expression of IL-1β, IL-6, and TNF-α in epididymal adipose tissue. Compared to the control group, mice fed the HFD showed the up-regulation in PPAR-γ, FAS, IL-6, and TNF-α genes, and down-regulation in CPT1 gene expression. FRF treatement markedly reduced the expression of PPAR-γ, FAS, IL-6, and TNF-α compared to those in HFD control, whereas increased the expression level of CPT1. Conclusions: These results suggest that the FRF and its major active constituent, rutin, can be used as effective anti-obesity agents.
Keywords
Morus alba; Anti-obesity; C57BL/6 Mice; Expression; Flavonoid; Gene; Mulberry Leaf; Rutin; 3T3-L1 Cell;
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