• Applied Biological Chemistry
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• v.49 no.1
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• pp.65-69
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• 2006

With extracted Aloe vera and Aloe arborescens, anti-inflammatory effect was examined in phosphatidic acid-stimulated Raw 264.7 cells. Phosphatidic acid $(50\;{\mu}g/ml)$ treatment increased inflammation related 264.7 cells. The extracts of $80\;{\mu}g/ml$ concentration from Aloe arborescens reduced the expression of iNOS. Aloe vera and Aloe arborescens also reduced the expression of COX-2, it seems that anti-inflammatory effects of Aloe extracts is partly due to the inhibition of COX-2 expression by inhibiting in Raw 264.7 cells.

• Applied Biological Chemistry
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• v.51 no.1
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• pp.44-48
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• 2008

With extracts from Cheongmoknosang, anti-inflammatory effect was examined in LPS-stimulated Raw 264.7 cells. LPS (10 ng/ml) treatment increased the production of inflammatory cytokines, $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ but the ethanol extracts from Cheongmoknosang slightly decreased the production of $TNF-{\alpha}$ and also reduced the expression of iNOS and the production of COX-2. It seems that anti-inflammatory effects of ethanol extracts from Cheongmoknosang is partly due to the inhibition of iNOS and COX-2 expression by inhibiting nuclear translocation of $NF-{\kappa}B$ and AP-l in Raw 264.7 cells.

• Journal of the Korean Applied Science and Technology
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• v.31 no.1
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• pp.23-30
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• 2014

In this study, a research of anti-inflammatory effect and application for cosmetics after extracting of germinated mung bean. Germinated mung bean extract was showed high NO constraining effect compared with $250{\mu}g/m{\ell}$ concentrated mung bean extract and germinated mung bean extract. Also shown is the highest nitrogen oxide inhibiting effect that using 1,3-butylene glycol when comparing the results of extraction conditions of a water-soluble, and extracted mung bean germinated for 5 days. The result of applying the germinated mung bean extract hairdye inflammatory reaction happens many showed nitrogen oxides higher inhibitory effect than that hair dye containing germinated mung bean extract is not included. Therefore, germinated mung bean extract is expected to the abirritant and anti-inflammatory agent as the material for cosmetic.

• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.391-395
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• 2015

We examined the anti-inflammatory effect and anti-tumor activity of Tetragonia tetragonioides crude extracts and fractions. The anti-inflammatory activity of T. tetragonioides was exuded through the inhibition of lipopolysaccharide (LPS, $1{\mu}g/ml$), induced nitric oxide (NO) and interleukin (IL)-$1{\beta}$ production. The production of IL-6 and tumor necrosis factor $(TNF)-{\alpha}$ also decreased in LPS induced RAW264.7 cells after treatment with polysaccharide (PS) fraction. Furthermore, the hexane (HX) fraction strongly inhibited the granulocytes macrophage-colony stimulating factor (GM-CSF) production. In ICR mice previously inoculated with Sarcoma 180, the life prolongation effects were 16.67% with an intraperitoneal injection of methanol (MeOH) extract and polysaccharide fraction at a dose of 100 mg/kg/day. The results are an important preliminary step toward the development of effective anti-inflammatory and anti-tumor agents using T. tetragonioides.

• Journal of Applied Biological Chemistry
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• v.65 no.3
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• pp.221-230
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• 2022

We investigated the antioxidant and anti-inflammatory effects of silymarin. The antioxidant activity was evaluated using 1,1-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals scavenging assays. Silymarin scavenged 71% of DPPH radicals and 78% of ABTS radicals at a concentration of 1 mg/mL, respectively. Silymarin effectively inhibited the oxidative damage of DNA, and the oxidative modifications of human serum proteins and Cu,Zn-SOD. Also silymarin effectively inhibited H₂O₂- and LPS-induced cell death as well as the generation of reactive oxygen species and DNA fragmentation by H₂O₂ and LPS. The results suggested that silymarin might be an effective natural antioxidant and anti-inflammatory material.

• Journal of the Korean Applied Science and Technology
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• v.41 no.2
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• pp.251-259
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• 2024

This study evaluated the potential of hemp seed oil (Cannabis sativa L. seed oil, CSO) and hemp seed oil complex (Cannabis sativa L. seed oil complex, CSOC) as an anti-inflammatory material through comparative analysis. Anti-inflammatory effects of CSO and CSOC were confirmed through lipopolysaccharide (LPS)-induced RAW264.7 model. As a result of confirming the inhibition of lipid oxidation through lipoxygenase inhibitory activity, CSO was not inhibited, but COSC was inhibited by more than 70%. As a result of confirming cytotoxicity through MTT analysis, CSO did not show cytotoxicity, but CSOC showed cytotoxicity at over 200 ㎍/ml. In LPS-induced RAW264.7, the expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) were significantly inhibited by CSOC compared to CSO. Additionally, CSOC significantly inhibited the expression of cyclooxygenase (COX)-2 and the production of prostaglandin E₂ (PGE₂). Through this study, we confirmed that CSOC has superior anti-inflammatory effects than CSO and has the potential to be used as an anti-inflammatory material.

• Korean Journal of Organic Agriculture
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• v.19 no.2
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• pp.245-253
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• 2011

This study was carried out to investigate the biological effects of organic soybean extracts. Cellular and molecular analysis was performed to determine anti-oxidative, anti-inflammatory, anti-apoptotic, and anti-inflammatory effects of soybean extracts. First, we obtained various solvent extracts of soybean such as water, ethanol, and methanol. Molecular and cellular analysis were performed with 0.1 mg/ml concentration of each solvent extracts. The results of anti-oxidative, antiinflammatory and anti-apoptotic effects of organic soybean extracts were prominent. However, organic soybean extracts were not observed in anti-allergic effects determined by releasing histamine from rat mast cell line, RBL-2H3. Conclusively, organic soybean suppress inflammatory responses. In addition, organic soybean could be applied as a functional food ingredient for treatment of chronic inflammation, asthma, and atopic dermatitis with enhanced anti-inflammatory activities.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.3 s.58
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• pp.173-180
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• 2006

Sesame (Sesamum indicum L.), one of the oldest oilseed crops, has been known to posses antioxidative and inflammatory effects. This seed contains lignan compounds such as sesamin, sesamol, sesaminol, sesaminol diglucosides (SDG), and sesaminol triglucosides (STG). Sesamin, a major lignan in sesame, displayed several biological activities including a protective effects against oxidative damage in the skin. In the present study, we investigated the effect of sesamin, sesamol, sesaminol, SDG, and STG, on nitric oxide (NO) induction and inducible nitric oxide synthane (iNOS) and cyclooxygenases-2 (COX-2) expression in lipopolysaccharides (LPS)-treated RAW 264.7 cells. The results showed that sesamol and sesaminol significantly inhibited NO generation but they were also cytotoxicity however, sesamin effectively inhibited NO production ($IC_{50}: 64{\mu}M$) without my cytotoxic effect in LPS-stimulated macrophage RAW 264.7 cells. In further study, it was founded that sesamin inhibited the expression of inducible nitric oxide synthase but not COX-2 expression. These results suggest that sesamin may be useful for improvements of the inflammatory diseases.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.1
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• pp.19-26
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• 2017

This study investigated the anti-inflammatory activities and cell viability of Amelanchier asiatica (A. asiatica) 70% ethanol extracts against RAW 264.7 cells induced by lipopolysaccharide (LPS). Cell toxicity test on macrophage cells (RAW 264.7) was performed by 3-[4,5-dimethyl-thiazol-2-yl]-2, 5-diphenyl-tetrazoliumbromide (MTT) assay and results showed 96% cell viability at $1,000{\mu}g/mL$ concentration. Anti-inflammatory activity was examined via the inhibitory tests on the production of LPS induced NO in RAW 264.7 cells by Griess assay. The result showed that the extract inhibited NO production in concentration dependent manner. The iNOS and COX-2 protein expression inhibitory effects were confirmed by western blot and by reverse transcription-polymerase chain reaction (RT-PCR). From the former they were decreased by 84.3%, 56.2% at $500{\mu}g/mL$ concentration, respectively, and from the latter decreased by 89.8%, 84.9% at $500{\mu}g/mL$, respectively. In conclusion, this study showed the anti-inflammatory effects of A. asiatica extracts. Thus, this could be applied to an anti-inflammatory agent.

• Journal of the Korean Applied Science and Technology
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• v.37 no.5
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• pp.1088-1099
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• 2020

The purpose of this study was to confirm the possiblility of Zingiber mioga as a cosmetic material. For this we carried out biologically activated material characteristic evaluation about anti-oxidant, anti-inflammatory, wrinkle reduction effects using Zingiber mioga extract. To carry out this experiment, we extracted Zingiber mioga extract from Zingiber mioga flowers exract (ZMF) and Zingiber mioga leaves exract (ZML) with 70% ethanol. In order to evaluate the anti-inflammatory effect, we tested the toxicity and the hindrance activity to nitric oxide of samples using macrophages (RAW 264.7 cells). After we measured DPPH radical scavenging activity, ABTS+ radical scavenging activity and superoxide dismutase (SOD)-like activity of the Zingiber mioga extracts, we knew that they increased depending on their concentration. ZMF showed higher antioxidant activity than ZML after the measurement of ABTS+ radial scavenging activity and superoxide desmutase (SOD)-like activity. According to the measurement result of Nitric oxide inhibition activity we knew that ZMF reduced NO productions in a concentration-dependent manner. After the measurement of the biosynthesis quantity of pro-collagen type-1, we knew that its excellent effect appeared 110% or more at the concentration of 25 ㎍/ml. And at the same concentration, the result of the measurement of metalloprotease (MMP)-1 inhibition effect showed the 20% activation. In conclusion, ZMF is expected to be applied as a cosmetic material for wrinkle reduction. Zingiber mioga is believed to be used as a natural cosmetic material because it has been proven to have antioxidant, anti-inflammatory, and wrinkle-improving effects.