• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.690-694
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• 2010

In this study, herbal wood vinegar including Bambusoideae, Cinnamomi Cortex, Zingiberis Rhizoma was tested to see possibility for cosmetic or skin related medicine. Anti-oxidation effect of herbal wood vinegar was tested by DPPH free radical scavenging activity, and showed 97% inhibition rate at $50{\mu}g/ml$. Anti-bacterial effect was tested by disc diffusion method, and it indicated strong anti-bacterial activity against normal skin flora Staphylococcus aureus. Whitening effect was measured by tyrosinase inhibition assay, and it was lower compared with vitamin C. Stability test was done by MTT assay, and cell toxicity was relatively high. Stability was also checked, and there was not significant change in color, aroma, appearance and pH during storage. Anti-atopic dermatitis test was done by hairless mouse and herbal wood vinegar recovered damaged skin to almost normal condition after 9 days of application. IgE concentration in herbal wood vinegar treated mouse was also reduced 30% compared with control. From the study, herbal wood vinegar showed good anti-oxidation, anti-bacterial and anti-atopic dermatitis effect, and had promising application in cosmetic or skin related medicine.

• Journal of Nutrition and Health
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• v.57 no.1
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• pp.53-64
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• 2024

Purpose: Mitochondria play a crucial role in preserving skeletal muscle mass, and damage to mitochondria leads to muscle mass loss. This study investigated the effects of oxypeucedanin hydrate, a furanocoumarin isolated from Angelica dahurica radix, on myogenesis and mitochondrial function in vitro and in zebrafish models. Methods: C2C12 myotubes cultured in media containing 0.1, 1, 10, or 100 ng/mL oxypeucedanin hydrate were immunostained with myosin heavy chain (MHC), and then multinucleated MHC-positive cells were counted. The expressions of markers related to muscle differentiation, muscle protein degradation, and mitochondrial function were determined by quantitative reverse transcription polymerase chain reaction. To investigate the effects of oxypeucedanin hydrate on mitochondrial dysfunction, Tg(Xla.Eef1a1:mito-EGFP) zebrafish embryos were treated with 5-fluorouracil, leucovorin, and irinotecan (FOLFIRI) with or without oxypeucedanin hydrate and analyzed for mito-EGFP intensity and mitochondrial length. Results: Oxypeucedanin hydrate significantly increased MHC-positive multinucleated myotubes (≥ 3 nuclei) and increased the expression of the myogenic marker myosin heavy chain 4. However, it decreased the expressions of muscle-specific RING finger protein 1 and muscle atrophy f-box (markers of muscle protein degradation). Furthermore, oxypeucedanin hydrate enhanced the expressions of markers of mitochondrial biogenesis (peroxisome proliferator-activated receptor-gamma coactivator 1 alpha, transcription factor a mitochondrial, succinate dehydrogenase complex flavoprotein subunit A, and cytochrome c oxidase subunit 1) and mitochondrial fusion (optic atrophy 1). However, it reduced the expression of dynamin-related protein 1 (a mitochondrial fission regulator). Consistently, oxypeucedanin hydrate reduced FOLFIRI-induced mitochondrial dysfunction in the skeletal muscles of zebrafish embryos. Conclusion: The study indicates that oxypeucedanin hydrate promotes myogenesis by improving mitochondrial function, and thus, suggests oxypeucedanin hydrate has potential use as a nutritional supplement that improves muscle mass and function.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.542-547
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• 2008

The anti-diabetic effects of water extracts (WE) from medicinal herbs on hepatic glucose-regulating enzymes, such as glucokinase (GCK), pyruvate dehydrogenase (PDH), acetyl-CoA carboxylase (ACC) and ${\alpha}$-glucosidase, were studied using the cytosol fraction in liver and mitochondia fraction in heart of a type II diabetic animal (GK rat, Goto-Kakizaki). The free radical scavenging activity of water extracts by DPPH method was also tested. We found that free radical scavenging activity was strong in Corni fructu (CF), Mokdan Bark (MDB), Chenhwabon (CHB) and Sanyack (SY), while that of Backbocreng (BBR), Shuckgihwang (SGH) and Taecsa (TS) was lower. For GCK activity in cytosol of liver, CF and CHB had a more effective activity than other extracts. PDH activity in mitochondria fraction of heart was higher in all of the extracts, expect for the TS extract, than in the control. ACC activity in cytosol fraction of liver was significantly higher in the CF, CHB, SGH, TS and SY extracts than in the control. CF, BBR and MDB led to a decrease in the ${\alpha}$-glucosidase activity. Therefore, these results suggest that all of the extracts may be used as functional material in the development as anti-diabetic functional food and medicine.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.2
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• pp.125-135
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• 2017

The purpose of this study is verification of the anti-oxidant effect and anti-inflammatory effect of Eggplant-cheongyeolsodokum composed of 8 herbs (Solanum melongena L., Lonicera japonica Thunb., Glycyrrhiza uralensis Fisch., Ligusticum chuanxiong Hort., Angelica gigas Nakai., Coptis deltoidea C. Y. Cheng et Hsiao., Gardenia jasminoides J. Ellis., Forsythia suspensa Vahl) to confirm the possibilities as useful cosmetic material. We used the modified prescription of 'cheongyeolsodokum' contained in Korean traditional medical book 'Donguibogam' as composition of Eggplant-cheongyeolsodokum and their proportions. Eggplant-cheongyeolsodokum were extracted with hot water, 70% ethanol and then powdered. To confirm anti-oxidant effect, we investigated radical scavenging ability (DPPH, $ABTS^+$, superoxide), superoxide dismutase (SOD)-like activity, total polyphenolic contents. Also to confirm anti-inflammatory effect, we investigated inhibition effect of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, and Inhibition effect of the expression of inflammatory-related proteins (iNOS, COX-2) by western blot analysis. As a result, Eggplant-cheongyeolsodokum showed good anti-oxidant and anti-inflammation effects, we suggest that it can be used as an active ingredient for cosmetics.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.2
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• pp.159-164
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• 2015

To identify new anti-inflammatory and whitening material, this study investigated the whitening and anti-inflammatory effects of the lyophilized powder from 6 oriental plant extracts (OPE; Citrus junos Tanaka, Mori cortex Radicis, Schisandra chinensis Baillon, Coix lachrymajobi var. mayuen, Angelica gigas NAKAI, and Sophora japonica L.) fermented with Streptococcus thermophilus by assessment of cytotoxicity on human dermal fibroblast, inhibitory effect of nitric oxide (NO) prodction, tyrosinase activity and melanin formation. The OPE was fermented with Streptococcus thermophilus at $37^{\circ}C$ for 2 days and the lyophilized powder was manufactured by freezing-dryer. OPE didn't show cytotoxicity at concentration of $500{\mu}g/mL$ using a cytopathic effect reduction method. OPE also exhibited inhibitory effect on nitric oxide (NO) prodction by Griess reagent system. Furthermore, OPE showed inhibitory effect on tyrosinase activity with dose dependent manner, and exhibited significant inhibition of melanin formation by measurement of melanin from culture media (p < 0.05). From these results, 6 OPE extracts showed anti-inflammatory and whitening effect and may be used as an active ingredient for cosmetics.

• Korean Journal of Food Science and Technology
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• v.44 no.6
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• pp.692-698
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• 2012

The chemical conversion pattern of salvianolic acid B (Sal B) in aqueous solution under different boiling conditions was compared. When the duration of boiling was varied, without varying temperature or pressure, the content of chemically converted compounds (CCCs) was mostly increased over time. In addition, under different conditions of temperature and pressure with the same boiling time, the content of a few compounds increased with increasing temperature and pressure. These results confirmed that high temperatures and pressures in boiling alter the final composition of CCCs of Sal B. Therefore, it was suggested that the boiling conditions (time, temperature, and pressure) may be responsible for alteration of biological activities of the compounds. Our investigation of the chemical conversion of compounds contained in foods and medicinal herbs may provide important information in clarifying the biological activity of Sal B containing foods and medicinal herbs.

• Food Industry
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• s.180
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• pp.27-64
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• 2004

국내 제조업 전체에서 식음료품은 $6.31\%$를 차지하고 있다. 2000년을 기준으로 식품산업의 총매출실적을 보면 32조 3,463억원을 기록하고 있는데, 이 중 식료품은 26조 921억원, 알콜올음료를 포함한 음료품은 6조 2,516억원을 차지하고 있다. 2001년, 음료시장의 규모는 약 2조 7,900억원 정도였다. 빠르게 성장하던 전체 음료시장의 매출은 IMF 이후 약간 주춤하는 경향을 보였지만 지속적으로 매출액이 상승하여 2002년에는 3조 4천억원의 매출을 기록하였다. 이것은 사이다, 후레바, 정통주스, 냉장주스, 스포츠 음료, 두유 및 기능성음료 등이 성장을 주도하였다. 2003년 상반기, 1조 6,500억원으로 전년 동기대비 $3\%$ 감소하였지만 2003년 전체 실적은 대략 지난해와 비슷한 3조 5,000억원 규모로 전망된다. 내수량의 경우 탄산음료와 과즙음료가 감소하는 경향을 나타냈지만, 전체적으로는 증가하였다. 다류시장의 규모는 약 650억원, 캔커피시장의 규모는 2,400억원 정도로 나타났다. 이처럼 음료$\cdot$다류 시장은 전체적으로 약 4조원의 거대시장규모를 형성하고 있으며 내수가 지속적으로 증가되고 있어, 점차 시장규모도 증가할 것으로 전망되고 있다. 최근에는 기능성 음료 및 다류의 시장규모가 전체의 $20\%$에 달할 정도로 신장하였으며, 기능성음료 및 다류의 특허출원도 지속적으로 증가하고 있는 실정이다. 이는 비단 국내에서 뿐만 아니라 세계적인 현상이며 이러한 추세는 앞으로 지속될 것으로 전망되고 있다. 이처럼 기능성음료 및 다류의 성장이 두드러지고 있는 것은 시대가 변하면서 현대인들의 생활방식과 식생활 변화, 질병형태 다양화 등 여러 사회적 여건이 변화되고 있는데 기인하며, 현대인의 건강에 대한 관심의 증대는 음료시장의 쾌속성장과 틈새수요를 지속적으로 창출하고 있다. 현재 세계 건강기능성 식품의 시장 규모는 약 160조원이며, 국내의 경우 약 1조원에 이르는 실정이다. 이 중 기능성음료 시장은 국내에서 약 4천억원 규모로 기능성식품 시장의 $40\%$를 차지하고 있다. 이처럼 기능성음료 시장은 최근 연간 $7\%$ 정도의 빠른 성장률을 보이고 있으며 향후에도 $6.7\%$의 실질 연간 성장률을 지속할 것으로 전망되고 있다. 전해질(이온)음료나 식이섬유를 포함하는 다이어트음료에서 출발한 기능성음료는 숙취해소, 성인병 예방, 스트레스 해소에 이르기까지 각종 질병의 치료 및 예방으로 확대되고 있다. 앞으로는 BT 및 나노 기술 등을 이용한 신기능 소재들에 대한 다각적인 효능의 규명에 따른 음료$\cdot$다류 소재의 개발과 더불어 체질개선, 다이어트, 숙취해소 등의 특정한 기능성과 관련한 음료$\cdot$다류의 특허출원이 계속될 것으로 전망된다. 복잡한 한약의 제조과정을 단순화하여 티백이나 캔 형태로 만든 맛과 기능이 조화된 음료$\cdot$다류 분야의 연구개발이 지속될 것으로 보이며, 시장 규모의 확대와 더불어 기능성과 간편성을 동시에 추구하는 신세대 소비자들의 성향을 겨냥하여 기존의 음료$\cdot$다류 전문 업체 외에도 제약회사 등의 비음료 업체도 다양한 형태의 음료$\cdot$다류 기술개발을 지속할 것으로 예상되고 있다. 특히 새로운 신기능성 소재를 개발하는 것은 고부가가치 기술로써 BT, NT 등의 최첨단 기술의 발전과 도입으로 인해 그동안 수입에 의존하던 기능성 소재들도 국내 기업들의 축전된 기반기술을 통하여 대거 참입할 것으로 보인다. 하지만, 여러 업체의 음료$\cdot$다류시장 진출에 따라 야기될 수 있는 비위생이고 효능이 불확실한 식품의 유통, 업체간의 과열경쟁에 따른 유통질서 문란 등은 모처럼 활기를 되찾은 음료$\cdot$다류 분야의 기술개발을 위축시킬 수도 있으므로 정부차원에서 체계적이고 효율적인 관리가 조속히 이루어져야 할 것이며 이를 제도적으로 뒷받침하기 위한 정책이 수립되고 시행되어야 할 것으로 보인다. 특히, 원료적인 측면에서의 무역 불균형은 반드시 해소되어야 할 부분이다. 대부분이 수입 기능성 원료에 의존하고 있는 현실에서 국내의 BT 기술 확충에 더욱 많은 투자와 노력이 집중되어야 할 것이다. 세계적으로 경쟁이 될 수 있는 기능성 소재의 개발과 이를 통한 신기능 음료의 개발은 단순한 수학적 계산을 넘어서 국가의 기술력을 홍보할수 있는 좋은 계기가 되기 때문이다. 바야흐로 음료$\cdot$다류 분야에 대한 열기가 식품시장을 주도하면서, 시장이 안정적으로 확대되기 위해서는 지속적인 기술개발이 이루어져야 한다는 것은 재론의 여지가 없다고 하겠다.

• Food Science and Preservation
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• v.12 no.3
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• pp.267-274
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• 2005

Oxidant stress is a well-known pivotal parameter for the degenerative immune diseases including asthma, atopic dermatitis, and rhinitis. In order to screen for anti-asthma agents effectively, we first established the infrastructure of high throughput screening(HTS) for anti-oxidant agents from agricultural products and/or oriental medicine library extracted with water, methanol, dimethyl sulfoxide, ethyl acetate and juice, Using the screening system, we found that Chaenomelis langenariae, Rhus javanica L., Camellia sinensis, Helianthus annuus and Angelica utilis Makino had strong anti-oxidant activity. Moreover, Helianthus annuus, Rehmannia glutinosa Libo and Angelica utilis Makino have protection activities by treatment of an oxidant hydrogen peroxide. Together, these results suggest that screened agents could be potential agents against asthma, although the in vivo studies should be clearly tested.

• Microbiology and Biotechnology Letters
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• v.20 no.5
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• pp.491-497
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• 1992

The water extract of Sinomeniiacuti Radix (Sinomenium acutum) was found out to have a strong inhibition activity on the growth of Clostridium perfringens. The anti-bacterial activity was stable at the range of pH 1 to pH 13 and kept in a thermal treatment at $121^{\circ}C$ for 15 minutes. The minmal inhibition concentration of the Sinomeniiacuti Radix extract on the growth of Cl. peifringens was 1000 ppm. The Sinomeniiacuti Radix extract also suppressed the growth of Cl. ramosum, Cl. paraputnficum, Cl. butyn'cum, Bifidobacterium blficum, Bacteriodes fragilis, Eubacterium limosum. The extract, however, did not inhibit the growth of Bif adolescentis, Blf. infantis, Bif longum, E. coli. Enterococcus faecalis and Staphylococcus aureus. On the other hand, the extract showed a promoting effect for the growth of Bif animalis and Lactobacillus acidophilus.

• Food Science and Preservation
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• v.17 no.2
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• pp.297-300
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• 2010

To evaluate whether active components produced by solid fermentation of Phellinus baumii and Ephedra sinica have potential in ameliorating allergic symptoms in mice, we tested anti-allergic activities in a dinitrofluorobenzene (DNFB)-induced allergic mouse model. DNFB-induced allergic symptoms werereduced to about 50% of control levels by active components produced by solid fermentation of Phellinus baumii and Ephedra sinica, as evaluated by measuring the width of epidermal swelling. H&E staining also revealed that these active components markedly reduced allergic symptoms in the epidermis of the ear. The results indicate that active components produced by solid fermentation of Phellinus baumii and Ephedra sinica have the potential to ameliorateallergic symptoms, and may be useful biomaterial(s) in the neutraceutical or cosmeceutical industry.