Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Study on Anti-oxidant and Anti-inflammatory Activity of Eggplant-cheongyeolsodokum

가지-청열소독음(淸熱消毒飮)의 항산화 및 항염 효능에 관한 연구

  • Yoon, Jong-Moon (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Dong-In (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Ji-Hae (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Han, So-Jung (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Ha-Eun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Hyeon-Jeong (Institute of Technology, Herbnoori Co., Ltd.) ;
  • Nam, Kyu-Woo (THANK YOU FARMER Co., Ltd.) ;
  • Park, Ji-Yeon (THANK YOU FARMER Co., Ltd.) ;
  • Chi, Gyeong-Yup (Department of Cosmeceutical Science, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
  • 윤종문 (대구한의대학교 화장품약리학과) ;
  • 김동인 (대구한의대학교 화장품약리학과) ;
  • 이지혜 (대구한의대학교 화장품약리학과) ;
  • 한소정 (대구한의대학교 화장품약리학과) ;
  • 김하은 (대구한의대학교 화장품약리학과) ;
  • 김현정 (주식회사 허브누리) ;
  • 남규우 (주식회사 땡큐파머) ;
  • 박지연 (주식회사 땡큐파머) ;
  • 지경엽 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2017.04.19
  • Accepted : 2017.06.27
  • Published : 2017.06.30
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Abstract

The purpose of this study is verification of the anti-oxidant effect and anti-inflammatory effect of Eggplant-cheongyeolsodokum composed of 8 herbs (Solanum melongena L., Lonicera japonica Thunb., Glycyrrhiza uralensis Fisch., Ligusticum chuanxiong Hort., Angelica gigas Nakai., Coptis deltoidea C. Y. Cheng et Hsiao., Gardenia jasminoides J. Ellis., Forsythia suspensa Vahl) to confirm the possibilities as useful cosmetic material. We used the modified prescription of 'cheongyeolsodokum' contained in Korean traditional medical book 'Donguibogam' as composition of Eggplant-cheongyeolsodokum and their proportions. Eggplant-cheongyeolsodokum were extracted with hot water, 70% ethanol and then powdered. To confirm anti-oxidant effect, we investigated radical scavenging ability (DPPH, $ABTS^+$, superoxide), superoxide dismutase (SOD)-like activity, total polyphenolic contents. Also to confirm anti-inflammatory effect, we investigated inhibition effect of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, and Inhibition effect of the expression of inflammatory-related proteins (iNOS, COX-2) by western blot analysis. As a result, Eggplant-cheongyeolsodokum showed good anti-oxidant and anti-inflammation effects, we suggest that it can be used as an active ingredient for cosmetics.

본 연구의 목적은 8가지 한약재(가지, 금은화, 감초, 천궁, 당귀, 황련, 치자, 연교)로 구성된 가지청열소 독음의 항산화 효과와 항염증 효과를 검증하여 효과적인 화장품소재로서의 가능성을 확인하는 것이다. 한약재의 구성과 비율은 동의보감에 수록된 청열소독음(淸熱蘇毒飮) 처방을 변형시켜 사용하였고 추출은 열수와 70% 에탄올로 하여 동결건조 분말화하였다. 항산화 효능을 확인하기 위해 라디칼 소거능력(DPPH, $ABTS^+$, superoxide), superoxide dismutase (SOD)유사활성능력, 총 폴리페놀 화합물 함량을 조사하였고 항염증 효능을 확인하기 위해 LPS (lipopolysaccharide)로 염증반응을 유도시킨 RAW264.7 대식세포에서의 nitric oxide(NO)생성 저해력과 western blot 분석을 통한 염증 관련 단백질 inducible nitric oxide synthase (iNOS)와 cyclooxygenase (COX-2)의 발현 저해를 확인하였다. 그 결과, 가지청열소독음은 뛰어난 항산화 효과와 항염증 효과를 나타내었고 화장품을 위한 효과적인 성분으로 사용 가능할 것으로 사료된다.

Keywords

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