• KSBB Journal
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• v.24 no.1
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• pp.1-8
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• 2009

In the past decade, we have observed rapid advances in the development of biochips in many fields including medical and environmental monitoring. Biochip experiments involve immobilizing a ligand on a solid substrate surface, and monitoring its interaction with an analyte in a sample solution. Metal nanoparticles can display extinction bands on their surfaces. These charge density oscillations are simply known as the localized surface plasmon resonance (LSPR). The high sensitivity of LSPR has been utilized to design biochips for the label-free detection of biomolecular interactions with various ligands. LSPR-based optical biochips and biosensors are easy to fabricate, and the apparatus cost for the evaluation of optical characteristics is lower than that for the conventional surface plasmon resonance apparatus. Furthermore, the operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in LSPR research and also describe the LSPR-based optical biosensor constructed with a core-shell dielectric nanoparticle biochip for its application to label-free biomolecular detections such as antigen-antibody interaction.

• Applied Chemistry for Engineering
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• v.20 no.6
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• pp.571-580
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• 2009

Biochip sensing technologies offering in-situ, fast and real-time measurements in addition to portability can be powerfully utilized in a wide spectrum of research areas including environmental science, food science, medical diagnostics and drug development. In this article, we introduce current research trends and economic aspects of the development of various optical biochip technologies for the analysis of organophosphorus/carbamate pesticides in environmental samples, which is of global importance with serious consequences for both current and future generations. In particular, we will highlight recent efforts made in the creation of highly sensitive and selective optical biochip sensors in conjunction with nanobiotechnologies and microfabrication for the rapid detection of organophosphorus/carbamate pesticides.

• Journal of Korea Technology Innovation Society
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• v.13 no.2
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• pp.252-281
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• 2010

In this paper, we analyze the result of the Technology Level Evaluation of 'Biochip and biosensor (BB) Technology' consisted of 3 sub-categorized technologies; biochip sensing (BS), lab on a chip and high-efficient customized health care technology. As an analysis tool, authors used a delphi (a repeated survey) and dynamic methodology with technology growth model to overcome limits of previous evaluations. As a result, levels of BB were evaluated 51.5% (Korea) and 75.1% (US), and the technology gap between two countries was 6.1 yrs. In 2013, these levels were expected to change to 60.1% (Korea), 78.4% (US) and 4.3 yrs, respectively. In comparison with other biotechnology, the gap of BB was smaller and expected to catch up with US faster. In the case of sub-categorized technologies, they showed the smallest gap and would have faster catch-up speed than other sub-categorized technologies in the Biotechnology field. Based on the result of the survey, relative superiority of BB in Korea was originated from competent researchers and research fund, but weak basic science would be weak points. We think that BB's characteristic as an emerging technology and concentrated research activities on BB are additional strong points. This research proposes the supporting and supplemented points to promote the BB in Korea.

• KSBB Journal
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• v.22 no.6
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• pp.371-377
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• 2007

Polyhydroxyalkanoates (PHAs) are a family of microbial polyesters that can be produced by fermentation from renewable resources. PHAs can be used as completely biodegradable plastics or elastomers. In this paper, novel applications of PHAs in biosensor are described. A general platform technology was developed by using the substrate binding domain (SBD) of PHA depolymerase as a fusion partner to immobilize proteins of interest on PHA surface. It could be shown that the proteins fused to the SBD of PHA depolymerase could be specifically immobilized onto PHA film, PHA microbead, and microcontact printed PHA surface. We review the results obtained for monitoring the specific interaction between the SBO and PHA by using enhanced green fluorescent protein, red fluorescent protein, single chain antibody against hepatitis B virus preS2 surface protein and severe acute respiratory syndrome coronavirus surface antigen as model proteins. Thus, this system can be efficiently used for studying protein-protein and possibly protein-biomolecule interactions for various biotechnological applications.

• Applied Chemistry for Engineering
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• v.29 no.6
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• pp.645-651
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• 2018

Lung cancer has the highest death rate of any cancer diseases in Koreans. However, patients often feel difficult to recognize their disease before facing the terminal diagnosis due to the absence of any significant symptoms. Furthermore, the clear detection of an early cancer stage is usually obscure with existing diagnostic methods. For this reason, extensive research efforts have been made on introducing a wide range of biochemical diagnostic tools for the molecular level analysis of biological fluids for lung cancer diagnoses. A chip-based biosensor, one type of the analytical devices, can be a great potential for the diagnosis, which can be used without any further expensive analytical equipments nor skilled analysts. In this mini review, we highlight recent research trends on searching biomarker candidates and bio-chip sensors for lung cancer diagnosis in addition to discussing their future aspects.

• IT SoC Magazine
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• s.16
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• pp.51-54
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• 2006

융합기술이 향후 미래를 이끌 핵심 기술로 떠오르고 있다. 특히 IT.BT 융합기술은 향후 새로운 기술혁명을 주도할 수 있는, 사회 전반에 파급효과가 큰, 세계경제의 신성장동력으로 주목받고 있다. IT.BT융합 기술은 바이오인포매틱스, 바이오전자, 생체정보인터페이스, 생체정보보호, 바이오컴퓨터 등으로 구분할 수 있고 그 영역이 매우 광범위하다. 여기에서는 바이오전자 가운데 융합 부품으로 구분할 수 있는 바이오칩과 바이오센서 분야의 사업동향을 살펴보고자 한다.

• KSBB Journal
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• v.20 no.2 s.91
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• pp.123-128
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• 2005

We have addressed two important issues of immunosensing biochips, including the construction of antibody functionalized suface for efficient affinity reactions and the development of a signal registration strategy that converts biospecific reactions into highly quantifiable electrochemical and/or optical signals. The developed immunoassay reaction is an integrated version of enzyme-mediated immunoprecipitaion reaction, which is widely used in immunohistochemistry, and electrochemical signaling reaction. For the evaluation of analytical performance of fabricated immunosensing biochips, signaling for mouse IgG in antiserum was conducted. Applications of the developed strategy have been found for the evaluation of histology chemicals and for the signal amplification for array-type biochip analysis.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.11
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• pp.25-35
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• 2000

• Information and Communications Magazine
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• v.25 no.6
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• pp.33-39
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• 2008

본고에서는 최신 과학기술 분야의 화두가 되고 있는 생명공학(BT)과 정보통신(IT)의 융합기술에 대해서 소개한다. 대표적인 기술로 바이오인포매틱스, 바이오칩, 바이오센서, 그리고 이들의 총체적인 기술인 유비쿼터스 헬스케어 시스템에 대해서 알아본다. BT, IT 융합기술을 통해 과학기술 분야에 새로운 연구분야와 효율적인 분석 기술을 제공해줌으로써, 인류의 건강에 큰 도움을 줄 것으로 기대된다.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.247-247
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• 2010

전계효과 트랜지스터를 이용한 바이오센서는 하나의 칩 위에 많은 센서 소자를 집적할 수 있으므로, 같은 종류의 센서를 다수 배열함으로써 다차원화할 수 있고, 다른 종류의 센서를 여러개 배열함으로써 다기능화할 수 있다. 또한 지능회로와 함께 집적하여 지능화하거나, 관련회로 및 장치들을 함께 집적함으로써 시스템화할 수 있기 때문에 최첨단 센서로 각광을 받고 있다. 그러나, 전계효과 트랜지스터를 이용한 바이오센서는 게이트 영역에 생체 분자를 고정시키는 것이 어렵고, 고정되더라도 생체 분자의 양이 미량이어서 재현성이 떨어지며, 생체 분자가 발생시키는 시그널이 적어 전류 세기 변화에 대한 검출감도가 저하되는 문제점이 있다. 본 연구에서는 반도체 리소그래피 공정을 이용하여 생체 분자를 물리 화학적 처리 없이 게이트 영역에 집중적으로 고정시킬 수 있는 기술에 대해 연구하였다. 산화막이 증착된 기판 위에 포토레지스트를 도포한 뒤 리소그래피공정을 이용하여 패터닝 하였으며 기판 위에 human embryonic kidney(HEK)-293 세포를 배양하였다. 연구결과, 친수성인 포토레지스트보다 소수성인 산화막 영역에 다수의 세포가 선택적으로 집중 배양됨을 확인하였다. 따라서 본 연구결과를 바이오센서에 적용할 경우 센서의 검출감도를 향상시킬 수 있을 것으로 기대된다.