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Integration of immunohistochemical reactions into Electrochemical and Optical Analyses of Biochips  

Choi Hyoung Gil (Department of Biotechnology, Ajou University)
Hong Eun Kyoung (Department of Anatomy, Chonnam National University Medical School)
Lee Seung-Won (Department of Anatomy, Chonnam National University Medical School)
Yoon Hyun C. (Department of Biotechnology, Ajou University)
Publication Information
KSBB Journal / v.20, no.2, 2005 , pp. 123-128 More about this Journal
Abstract
We have addressed two important issues of immunosensing biochips, including the construction of antibody functionalized suface for efficient affinity reactions and the development of a signal registration strategy that converts biospecific reactions into highly quantifiable electrochemical and/or optical signals. The developed immunoassay reaction is an integrated version of enzyme-mediated immunoprecipitaion reaction, which is widely used in immunohistochemistry, and electrochemical signaling reaction. For the evaluation of analytical performance of fabricated immunosensing biochips, signaling for mouse IgG in antiserum was conducted. Applications of the developed strategy have been found for the evaluation of histology chemicals and for the signal amplification for array-type biochip analysis.
Keywords
Biochip; biosensor; immunohistochemistry; immunoassay; electrochemical;
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1 Pemberton, R. M., J. P. Hart, and T. T. Mottram (2001), An electrochemical immunosensor for milk progesterone using a continuous flow system, Biosens. Bioelectron. 16,715-723   DOI   ScienceOn
2 Yoon, H. C, H. S. Yang, and S. Y. Byun (2004), Ferritin immunosensing on microfabricated electrodes based on the integration of immunoprecipitation and electrochemical signaling reactions, Anal.Sci. 20, 1249-1253   DOI   ScienceOn
3 Kobori, A., S. Horie, H. Suda, I. Saito, and K. Nakatani (2004), The SPR Sensor Detecting Cytosine-Cytosine Mismatches, J. Am.Chem. Soc. 126, 557-562   DOI   ScienceOn
4 Miyachi, H., A. Hiratsuka, K. Ikebukuro, K. Yano, H. Mugururna, and I. Karube (2000), Application of polymer-embedded proteins to fabrication of DNA array, Biotechnol. Bioeng. 69, 323-329   DOI   ScienceOn
5 Anzai, J. I., Y. Kobayashi, N. Nakamura, M. Nishimura, and T. Hoshi (1999), Layer-by-Iayer construction of multilayer thin films composed of avidin and biotin-labeled poly(amine)s, Langmuir. 15,221-226   DOI   ScienceOn
6 Yoon, H. C., M. Y. Hong, and H. S. Kim (2000), Affinity biosensor for avidin using a double functionalized dendrimer monolayer on a gold electrode, Anal. Biochem. 282, 121-128   DOI   ScienceOn
7 Yoon, H. C., H. S. Yang, and Y. T. Kim (2002), Biocatalytic precipitation induced by an affinity reaction on dendrimer-activated surfaces for the electrochemical signaling form immunosensors, Analyst. 127, 1082-1087   DOI   ScienceOn
8 Wang, G., C. L. Achim, R. L. Hamilton, C. A. Wilry, and V. Soontornniyomkij (1999), Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy, Methods. 18, 459-464   DOI   ScienceOn
9 Hong, M. Y., H. C. Yoon, and H. S. Kim (2003), Protein-ligand interactions at the PAMAM dendrimers monolayers on gold, Langmuir. 19, 416-421   DOI   ScienceOn
10 Lee, W., B. S. Chun, B. K. Oh, W. H. Lee, and J. W. Choi (2004), Fabrication of protein A-Viologen hetero Langmuir-Blodgett fum for fluorescence immunoassay, Biotechnol. Bioprocess Eng. 9, 241-244   DOI   ScienceOn
11 Yoon, H. C., J. S. Ko, H. S. Yang, H. B. Pyo, K. H. Chung, S. J. Kim, and Y. T. Kim (2003), A polymer-based microfluidic device for immunosensing biochips, Lab on a Chip. 3, 106-113   DOI   ScienceOn
12 Kim, N. S., I. S. Park, and D. K. Kim (2004), Characteristics of a label-free piezoelectric immunosensor detecting Pseudomonas aeruginosa, Biosens. Bioelectron. 18, 797-804   DOI   ScienceOn
13 Liu, Z., and M. D. Amiridis (2004), FT-IRRAS spectroscopic studies of the interaction of avidin with biotinylated dendrimer surfaces, Colloids Surf. B: Biointerfaces 35, 197-203   DOI   ScienceOn
14 Wang, J. (1998), DNA biosensors based on peptide nucleic acid (PNA) recognition layer, Biosens. Bioelectron. 13, 757-762   DOI   ScienceOn
15 McDonald, J. C., D. C. Duffy, J. R. Anderson, D. T. Chiu, H. Wu, O. J. A. Schueller, and G. M. Whitesides (2000), Fabrication of microfluidic systems in poly(dimethylsiloxane), Electrophoresis. 21,27-40   DOI   ScienceOn
16 Kim, U. R. (2003), The theory and application of piezoelectric quartz crystal microbalance(PZ QCM), Korean J. Biotechnol.Bioeng. 18, 79-89
17 Vlies, D., K. W. A. Wirtz, and E. H. W. Pap (2001), Detection of protein oxidation in Rat-1 fibroblasts by fluorescently labeled tyramide, Biochemistry. 40, 7783-7788   DOI   ScienceOn
18 Wordinger, R., G. Miller, and D. Nicodemus (1987), Manual of immunoperoxidase techniques., p23-43, American Soc. Clin. Patho!. Press, Chicago
19 Pyo H. B., , Y. B. Shin, M. G. Kim, and H. C. Yoon (2004), Multichannel surface plasmon resonance imaging and analysis of micropatterned self-assembled monolayers and protein affinity interactions, Langmuir. 21, 166-171   DOI   ScienceOn
20 Liu, Y., C. D. Gasrcia, and C. S. Henry (2003), Recent progress in the development of $\mu$-TAS for clinical analysis, Analyst. 128, 1002-1008   DOI   ScienceOn