• Research in Plant Disease
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• v.13 no.3
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• pp.152-156
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• 2007

Incidences of leaf blight of lily cultivars Raizan and Casa Blanca in the open field cultivation were 50% and 45.4%, respectively, while those in the green house cultivation were significantly reduced to 1.5% and 1.9%, respectively, In the green house, the incidences of the disease in sprinkler watering cultivation were $14.5{\sim}16.5%$, while those in drip watering cultivation were only $1.5{\sim}2%$. Incidence of the disease was severe in the field where the lily was cultivated successively for 2 to 3 years. Isolation frequencies of B. elliptica from overwintered plant debrises such as leaves, stems, capsules, and bulbs were 43.3%, 46.7%, 60% and 0%, respectively, while those of B. cinerea were 10.3%, 0%, 3.3% and 0%, respectively, Incidence of leaf blight in the field where diseased plant debris was cleaned was 7.3%, while that in the field where diseased plant debris was not cleaned was 56.5%. Incidences of the disease in the field where coverages of soil surface with black vinyl, bark or rice straw were used were 6.6%, 8.2% and 11.3%, respectively, while that in the field where the coverage was not used was 21.3%.

• Research in Plant Disease
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• v.20 no.2
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• pp.79-86
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• 2014

In order to seek more eco-friend, economic and safer quarantine method than current methyl bromide fumigation, the convergent treatment with 200 Gy of gamma irradiation and several chemicals such as nano-siver particles (NSS), sodium dichloroisocyanurate (NaDCC) was tried on the cuttings of lily in the packing of catonnage box for export. With 6 independent experiments of gamma irradiation on the three lily cultivars, cvs. Siberia, Le reve and Sorbonne, incidence and severity of lily leaf blight was investigated on leaves and petals at 8-d after infection. 200 Gy of gamma irradiation decreased at 13-25% of severity on the leaf of Sorbonne, but it increased at 2-5% of severity on the leaf of Siberia and Le reve. Chemical substitutes such as NSS and NaDCC were not effective to control of lily blight on cuttings. By 200 Gy of gamma irradiation treatment, chlorophyll contents were statistically significantly decreased at 12-d after irradiation and the longevities vaselife of fully open flower of Siberia and Sorbonne were increased at 0.4 to 1.2 days. In addition, the relative fresh weights of the gamma irradiated cuttings were severely dried compared to the non-irradiated control. On the other hands, the symptoms of phyto-toxicity of high dose gamma irradiation at 1 or 2 kGy on cv. Siberia were to be blight at the tip of bloom, bent necks of flower, and delayed the process of flowering.

• Research in Plant Disease
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• v.12 no.3
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• pp.260-266
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• 2006

Pythium blight caused by Pythium spp. is one of major diseases in putting green of golf course. In this study, microorganisms which are anatgonistic to Pythium aphanidermatum, a pathogen of pythium blight, were selected primary through in vitro tests, dual culture method and triple layer agar diffusion method. In vivo test against pythium blight were conducted to select the best candidate biocontrol microorganism by pot experiment in a plastic house. Bacillus subtilis GB-0365 was finally selected as a biocontrol agent against pythium blight. Relative Performance Indies(RPI) was used as a criterion of selecting potential biocontrol agent. B. subtilis GB-0365 showed resistance to major synthetic agrochemicals used in golf course. Alternative application of synthetic agrochemicals and B. subtilis GB-0365 was most effective to successfully contol pythium blight. B. subtilis GB-0365 suppressed the development of pythium bight of bentgrass by 56.4% as compared to non-treated control and its disease control efficacy was 60.9% of a synthetic fungicide Oxapro(WP) efficacy. B. subtilis GB-0365 has a potential to be a biocontrol agent for control of pythium blight.

• Research in Plant Disease
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• v.19 no.2
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• pp.84-89
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• 2013

The incidence factors of Rice stripe virus (RSV) were analyzed by studying the population density and the viruliferous insect rate (VIR) of small brown planthopper (SBPH), the incidence of stripe disease, alternate host, and susceptible cultivar in Chungnam Province. The population of overwintering SBPH had been decreasing, but the VIR of overwintering SBPH had not been differing for three years, 2008 to 2010. No RSV was detected in the natural host plants, such as short awn, annual bluegrass, and barley. In 2009, relatively large population of SBPH with the VIR of 5.4% migrated from China. However, there was no evidence relating of migration large amount of SBPH from China in 2008 and 2010. Also the infection rate of RSV in rice was less than 1% in these periods. The cultivation area of the susceptible varieties had steadily decreased from 41% to 19% from 2007 to 2009. Therefore, the reduction factors of rice stripe disease in Chungnam Province with higher influx of inoculum could be with an appropriate forecasting and chemical control, cultivation of resistant varieties, changes in the cropping system, and the low winter-spring temperature.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.3
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• pp.579-588
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• 2015

Recent climage changes have resulted in increases in rainfall intensity and flood frequency as well as the risk of flood damage due to typhoons during the summer season. Water-friendly facilities such as ecological parks and sports facilities have been established on floodplains of rivers since the river improvement project was implemented and increases in the flood levels of rivers due to typhoons can lead to direct flood damage to such facilities. To analyze the hydraulic influence of these water-friendly facilities on floodplains or to evaluate their stability, numerical analysis should be performed in advance. In addition, it is crucial to address the drying and wetting processes generated by water level fluctuations. This study uses a Nays2D model, which analyzes drying and wetting, to examine its applicability to simple terrain in which such fluctuations occur and to natural rivers in which drying occurs. The results of applying this model to sites of actual typhoon events are compared with values measured at water level observatories. Through this comparison, it is determined that values of coefficient of determination ($R^2$), mean absolute error (MAE), and root-mean-square error (RMSE) are 0.988, 0.208, and 0.239, respectively, thus showing a statistically high correlation. In addition, the results are used to calculate flood risk indices for evaluation of such risk for water-friendly facilities constructed on floodplains.

• Research in Plant Disease
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• v.15 no.3
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• pp.202-208
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• 2009

The genetically modified leaffolder-resistant (Cry1Ac1) rice plant was evaluated for the changes of resistance by comparing the occurrence of major diseases with a japonica type Korean rice variety, Nakdong which was the mother plant of the transgenic rice event, in greenhouse and field conditions. There was no difference in the occurrence of sheath blight and Helminthosporium blight between the two varieties in the fields. We couldn't find any difference of resistance for fungal blast and bacterial leaf blight by artificial inoculation in greenhouse. There was also no difference in the susceptibility to sheath blight in artificial inoculation tests confirming the results in the fields. The possibility of gene transfer of Bar and Cry1Ac1 from the genetically modified rice plant to naturally infected pathogens such as Fusarium moniliforme and Pyricularia oryzae in the field conditions was tested by PCR. And the possible transfer of those genes by continuous inoculation of Xanthomonas oryzae pv. oryzae and Rhizoctonia solani was also tested. However, we couldn't find any possibility of transfer of the genes in natural and artificial conditions.

• Korean Journal of Organic Agriculture
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• v.18 no.1
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• pp.75-82
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• 2010

The purpose of this study is to investigate an occurrence rate of rice stripe virus disease under different rice transplanting time and different rice cultivars. After final harvest, the yield of rice infected by rice stripe virus was obtained on Wangchal cultivar compared to uninfected rice. The results obtained as following: 1) Yield of rice infected by rice stripe virus was decreased by 64% with reduction of culm length, pancile length, number of spike, number of spikelet, and grain filling compared to uninfected rice, 2) An occurrence rate of rice stripe virus disease under different rice planting season was high as order of May 30 > June 15 > July 1. In additional, to reduce a rice stripe virus disease on higher disease occurrence region and susceptible cultivars, rice planting season will be accepted on and after June 15, and 3) Eighteen cultivars were resistance cultivars such as Hwayeongbyeo, Onnuri, Ilmibyeo, Nampyongbyeo, Dongjin2ho, Hopumbyeo, Hwangkeumnuri, Malgeumi, Saenuri, Pungmi1ho, Haechanmulgyeo, Hwangkeumnodeul, Chilbobyeo, Dongjinbyeo, Younganbyeo, Junambyeo, Samkwangbyeo, and Nakdongbyeo. Twenty-one cultivars were susceptible cultivars such as Odaebyeo, Unkwangbyeo, Shinungbong1ho, Manabyeo, Hopyongbyeo, Borami, Damibyeo, Hwangchalbyeo, Sulgengbyeo, Nongrim6ho, Sasanishigi, Yunishigari, Chungmubyeo, Dongjin1ho, Huknambyeo, Guromy, Shanghehanghulna, Heukchalbyeo, Heukhwangbyeo, and Aranghwangchalbyeo.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.124-133
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• 2011

Bacterial blight (BB), cuased by the vascular pathogen Xanthomonas oryzae pv. oryzae, is one of the major threats in rice fields worldwide. In Korea, two resistance genes against BB, Xa1 and Xa3 had been intensively used for developing high quality japonica rice cultivars. Those traditional resistance sources have being rapidly ified by the adopting of BB pathogen through mutations of the corresponding avr-genes, such as K3a exhibiting high compatibility to both Xa1 and Xa3. To expanding genetic resource against BB in Korea, the Suweon506, an introgression line between a Korean japonica cultivar, Hwaseong and a wild relative, Oryza minuta, was be subjected for genetic analysis owing to the BB resistance. Through association analyses between the pathotyping and genotyping results for each $F_2$ progenies, derived from a cross between Suweon506 and a Tongil type cultivar, Milyang23, a major resistant dominant gene is localized on the subterminal region of rice chromosome 4, where at least three BB resistancde genes, Xa1, Xa2, and Xa22, were reported previously.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.101-104
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• 2016

Curvularia leaf blight of creeping bentgrass (Agrostis stolonifera) putting green by caused Curvularia trifolii was observed in Hapcheon, Korea. In July to September 2014, curvularia leaf blight developed on leaf blades of creeping bentgrass as small water-soaked lesions that subsequently turned into dark-colored, necrotic spots. The spots were expanded and became gray, grayish-brown, or light brown, circular to oblong lesions with purple to dark brown borders that often were surrounded by a yellow halo. The necrotic lesions coalesced, became irregular in shape and caused tip or complete blighting of the leaves. Blighted leaf blades appeared grayish-white to tan. The fungus was identified by morphological characters and 16S rDNA sequencing as C. trifolii. Conidia of the pathogen were short, with predominantly 3-septa, straight or often curved, with end cells frequently paler than intermediate cells. Size of the 3-septate conidia in culture are $26{\sim}28{\times}11{\sim}12{\mu}m$. Pathogenicity of the fungus was proved by artificial inoculation on the host. This is the first report of C. trifolii causing leaf blight on creeping bentgrass in Korea.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.457-463
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• 2011

Plant specific gene family, NAC (NAM, ATAF, and CUC) transcription factors have been characterized for their roles in plant growth, development, and stress tolerance. In this study, we isolated OsNAC69 gene and analyzed expression level by inoculation of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). NAC transcription factor family can be divided into five groups (I-V). On the basis of phylogenetic analysis, OsNAC69 was fall into group II. OsNAC69 was strongly induced 1 hr after infected with Xoo. To investigate its biological function in the rice, we constructed vector for overexpression in rice, and then generated transgenic rice lines. Gene expression of OsNAC69-overexpressed transgenic rice lines were analyzed by northern blot. Analysis of disease resistance to pathogen Xoo, nine OsNAC69-overexpressed transgenic rice lines showing high expression level of OsNAC69 were shown more resistant than wild type. These results suggest that OsNAC69 gene may play regulatory role during pathogen infection.