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http://dx.doi.org/10.5423/RPD.2009.15.3.202

Evaluation of Disease Resistance of a Leaffolder-resistant (Cry1Ac1) Rice Event and Gene Transfer to Plant Pathogens  

Nam, Hyo-Song (Division of Biotechnology, Chonbuk National University)
Shim, Hong-Sik (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Yu, Sang-Mi (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Lee, Se-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Kwon, Soon-Jong (Biosafety Division, National Academy of Agricultural Science, RDA)
Kim, Myung-Kon (Bio Food Technology, Chonbuk National University)
Lee, Yong-Hoon (Division of Biotechnology, Chonbuk National University)
Publication Information
Research in Plant Disease / v.15, no.3, 2009 , pp. 202-208 More about this Journal
Abstract
The genetically modified leaffolder-resistant (Cry1Ac1) rice plant was evaluated for the changes of resistance by comparing the occurrence of major diseases with a japonica type Korean rice variety, Nakdong which was the mother plant of the transgenic rice event, in greenhouse and field conditions. There was no difference in the occurrence of sheath blight and Helminthosporium blight between the two varieties in the fields. We couldn't find any difference of resistance for fungal blast and bacterial leaf blight by artificial inoculation in greenhouse. There was also no difference in the susceptibility to sheath blight in artificial inoculation tests confirming the results in the fields. The possibility of gene transfer of Bar and Cry1Ac1 from the genetically modified rice plant to naturally infected pathogens such as Fusarium moniliforme and Pyricularia oryzae in the field conditions was tested by PCR. And the possible transfer of those genes by continuous inoculation of Xanthomonas oryzae pv. oryzae and Rhizoctonia solani was also tested. However, we couldn't find any possibility of transfer of the genes in natural and artificial conditions.
Keywords
Event; Gene transfer; GMO; Leaffolder;
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1 Lorenz, M. G. and Wackernagel, W. 1994. Bacterial gene transfer by natural genetic transformation in the environment. Microbiol. Rev. 58: 563-602   PUBMED
2 Nielsen, K. M., van Weerelt, D. M., Berg, T. N., Bones, A. M., Hagler, A. N. and van Elsas, J. D. 1997. Natural transformation and availability of transforming DNA to Acinetobacter calcoaceticus in soil microcosms. Appl. Environ. Microbiol. 63: 1945-1952   PUBMED
3 Smalla, K., Wellington, E. M. H. and van Elsas, J. D. 1997. Natural background of bacterial antibiotic resistance genes in the environment. In: Nordic Seminar on Antibiotic Resistance Marker Genes and Transgenic Plants, pp. 43-57. Norwegian Biotechnology Advisory Board, Oslo
4 Widmer, F., Seidler, R. J., Donegan, K. K. and Reed, G. L. 1997. Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol. 6: 1-7   DOI   ScienceOn
5 Courvalin, P. 1995. Gene transfer from bacteria to mammalian cells. C. R. Acad. Sci. Ser. III Sci. Vie. 318: 1207-1212
6 Doolittle, R. F., Feng, D. F., Anderson, K. L. and Alberro, M. R. 1990. A naturally occurring horizontal gene transfer from a eukaryote to a prokaryote. J. Mol. Evol. 31: 383-388   DOI   PUBMED
7 Gebhard, F. and Smalla, K. 1998. Transformation of Acinetobacter sp. BD413 by transgenic sugar beet DNA. Appl. Environ. Microbiol. 64: 1550-1554   PUBMED
8 Heinemann, J. A. and Sprague, G. F. 1989. Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast. Nature 340: 205-209   DOI   ScienceOn
9 Mazodier, P., Petter, R. and Thompson, C. 1989. Intergeneric conjugation between Escherichia coli and Streptomyces species. J. Bacteriol. 171: 3583-3585   DOI   PUBMED
10 Smith, M. W., Feng, D.-F. and Doolittle, R. F. 1992. Evolution by acquisition: the case for horizontal gene transfers. Trends Biochem. Sci. 17: 489-493   DOI   ScienceOn
11 Nielsen, K. M., Gebhard, F., Smalla K., Bones, A. M. and Van Elsas, J. D. 1997. Evaluation of possible horizontal gene transfer from transgenic plants to the soil bacterium Acinetobacter calcoaceticus BD413. Theor. Appl. Genet. 95: 815-821   DOI   ScienceOn
12 Broer, I., Droge-Laser, W. and Gerke, M. 1996. Examination of the putative horizontal gene transfer from transgenic plants to agrobacteria. In: Transgenic Organisms and Biosafety (Schmidt, E.R. and Hankeln, T., Eds.), pp. 67-70. Springer Verlag, Berlin
13 Ye, G. Y., Yao, H. W., Shu, Q. Y., Cheng, X., Hu, C., Xia, Y. W., Gao, M. W. and Altosaar, I. 2003. High levels of stable resistance in transgenic rice with a cry1Ab gene from Bacillus thuringiensis Berliner to rice leaffolder, Cnaphalocrocis medinalis (Guenee) under field conditions. Crop protection 22: 171-178   DOI   ScienceOn
14 Trieu-Cuot, P., Carlier, C. and Courvalin, P. 1987. Plasmid transfer by conjunction from Escherichia coli to gram-positive bacteria. FEMS Microbiol. Lett. 48: 289-294   DOI
15 Khanna, M. and Stotzky, G. 1992. Transformation of Bacillus subtilis by DNA bound on montmorillonite and effect of DNase on the transforming ability of bound DNA. Appl. Environ. Microbiol. 58: 1930-1939   PUBMED
16 Hooykaas, P. J. J. 1989. Transformation of plant cells via Agrobacterium. Plant Mol. Biol. 13: 327-336   DOI   PUBMED   ScienceOn
17 Smith, J. M., Dowson, C. G. and Spratt, B. G. 1991. Localized sex in bacteria. Nature 349: 29-31   DOI   ScienceOn
18 Davies, J. 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science 264: 375-382   DOI   PUBMED