Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Evaluation of Disease Resistance of a Leaffolder-resistant (Cry1Ac1) Rice Event and Gene Transfer to Plant Pathogens

혹명나방 저항성벼(Cry1Ac1)의 병해 저항성 및 병원균으로의 유전자 전이

  • Nam, Hyo-Song (Division of Biotechnology, Chonbuk National University) ;
  • Shim, Hong-Sik (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Yu, Sang-Mi (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Se-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kwon, Soon-Jong (Biosafety Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Myung-Kon (Bio Food Technology, Chonbuk National University) ;
  • Lee, Yong-Hoon (Division of Biotechnology, Chonbuk National University)
  • 남효송 (전북대학교 환경생명자원대학 생명공학부) ;
  • 심홍식 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 유상미 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이세원 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 권순종 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 김명곤 (전북대학교 환경생명자원대학 바이오식품공학과) ;
  • 이용훈 (전북대학교 환경생명자원대학 생명공학부)
  • Published : 2009.12.01
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Abstract

The genetically modified leaffolder-resistant (Cry1Ac1) rice plant was evaluated for the changes of resistance by comparing the occurrence of major diseases with a japonica type Korean rice variety, Nakdong which was the mother plant of the transgenic rice event, in greenhouse and field conditions. There was no difference in the occurrence of sheath blight and Helminthosporium blight between the two varieties in the fields. We couldn't find any difference of resistance for fungal blast and bacterial leaf blight by artificial inoculation in greenhouse. There was also no difference in the susceptibility to sheath blight in artificial inoculation tests confirming the results in the fields. The possibility of gene transfer of Bar and Cry1Ac1 from the genetically modified rice plant to naturally infected pathogens such as Fusarium moniliforme and Pyricularia oryzae in the field conditions was tested by PCR. And the possible transfer of those genes by continuous inoculation of Xanthomonas oryzae pv. oryzae and Rhizoctonia solani was also tested. However, we couldn't find any possibility of transfer of the genes in natural and artificial conditions.

유전자 변형 혹명나방 저항성벼의 주요 병해에 대한 저항성 변화를 온실과 포장에서 모본으로 사용된 낙동벼와 비교하였다. 포장에서 벼잎집무늬마름병과 벼깨씨무늬병의 발병 정도는 큰 차이가 없었다. 온실에서 인위적으로 병원균을 접종하여 벼도열병과 흰잎마름병에 대해 저항성 변화여부를 조사한 결과에서도 두 품종간에 큰 차이를 보이지 않았고, 인위접종한 벼잎집무늬마름병에 대한 감수성도 두 품종간에 비슷하여 포장에서의 결과와 같은 경향을 보였다. 형질전환 벼의 제초제 저항성 유전자(Bar 유전자)와 혹병나방 저항성유전자(Cry1Ac1 유전자)가 병원균으로 전이되는가의 여부를 조사하기 위하여 포장에서 발병한 도열병과 키다리병원균을 분리한 후 DNA를 추출하여 PCR을 실시한 결과 두 유전자 모두 병원균으로 전이되지 않은 것으로 확인되었다. 또한, 병원균과 저항성벼의 지속적인 접촉에 의한 유전자 전이 가능성을 확인하기 위하여 잎집무늬마름병균과 흰잎마름병균을 계대 접종한 후 DNA를 분리하여 조사한 결과에서도 저항성 유전자의 전이는 일어나지 않은 것으로 확인되어, 본 실험에서는 자연상태와 인위적인 조건 모두에서 유전자 전이를 찾아 볼 수 없었다.

Keywords

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