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Generation of Bacterial Blight Resistance Rice with Transcription Factor OsNAC69-overexpressing  

Park, Sang Ryeol (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Cha, Eun-Mi (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Moon, Seok Jun (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Shin, Dongjin (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Hwang, Duk-Ju (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Ahn, Il-Pyung (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Bae, Shin-Chul (Bio-crop Development Div., National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Korean Journal of Breeding Science / v.43, no.5, 2011 , pp. 457-463 More about this Journal
Abstract
Plant specific gene family, NAC (NAM, ATAF, and CUC) transcription factors have been characterized for their roles in plant growth, development, and stress tolerance. In this study, we isolated OsNAC69 gene and analyzed expression level by inoculation of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). NAC transcription factor family can be divided into five groups (I-V). On the basis of phylogenetic analysis, OsNAC69 was fall into group II. OsNAC69 was strongly induced 1 hr after infected with Xoo. To investigate its biological function in the rice, we constructed vector for overexpression in rice, and then generated transgenic rice lines. Gene expression of OsNAC69-overexpressed transgenic rice lines were analyzed by northern blot. Analysis of disease resistance to pathogen Xoo, nine OsNAC69-overexpressed transgenic rice lines showing high expression level of OsNAC69 were shown more resistant than wild type. These results suggest that OsNAC69 gene may play regulatory role during pathogen infection.
Keywords
Rice; Xanthomonas oryzae pv. oryzae; OsNAC69; Bacterial blight resistance; Transcription factor;
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1 Collinge M, Boller T, 2001. Differential induction of two potato genes, Stprx2 and StNAC, in response to infection by Phytophthora infestans and to wounding. Plant Mol Biol 46:521-529.   DOI   ScienceOn
2 Dai LY, Liu XL, Xiao YH, Wang GL. 2007. Recent advances in cloning and characterization of disease resistance genes in rice. Journal of Integrative Plant Biology 49(1):112-119.   DOI   ScienceOn
3 David O, Liu N, Ronald PC, Bogdanove AJ. 2006. Xanthomonas oryzae pathovars: model pathogens of a model crop. Molecular Plant Pathology 7(5):303-324.   DOI   ScienceOn
4 Duval M, Hsieh TF, Kim SY, Thomas TL. 2002. Molecular characterization of AtNAM: a member of the Arabidopsis NAC domain superfamily. Plant Mol Biol 50:237-248.   DOI   ScienceOn
5 Ernst HA, Olsen AN, Larsen S, Lo Leggio L. 2004. Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Rep 5:297-303.   DOI   ScienceOn
6 Fang Y, You J, Xie K, Xie W, Xiong L. 2008. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice. Mol Gen Genet 280(6):547-563.   DOI   ScienceOn
7 He XJ, Mu RL, Cao WH, Zhang ZG, Zhang JS, Chen SY. 2005. AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. Plant J 44:903-916.   DOI   ScienceOn
8 Hegedus D, Yu M, Baldwin D, Gruber M, Sharpe A, Parkin I, Whitwill S, Lydiate D. 2003. Molecular characterization of Brassica napus NAC domain transcriptional activators induced in response to biotic and abiotic stress. Plant Mol Biol 53:383-397.
9 Hiei Y, Ohta S, Komari T, Kumashiro T. 1994. Efficient transformation of rice(Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6(2):271-282.   DOI   ScienceOn
10 Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L. 2006. Overexpressing a NAM, ATAF, and CUC(NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA 103:12987- 12992.   DOI   ScienceOn
11 Hussain SS, Kayani MA, Amjad M. 2011. Transcription factors as tools to engineer enhanced drought stress tolerance in plants. Biotechnology Progress 27:297-306.   DOI
12 Jiang J, Linscombe SD, Wang J, James Oard H. 2000. High Efficiency Transformation of U.S. Rice Lines from Mature Seed-Derived Calli and Segregation of Glufosinate Resistance under Field Conditions. Crop Sci 40:729-1741.
13 Kauffman HE, Reddy APK, Hsieh SPY, Merca SD. 1973. An improved technique for evaluation of resistance of rice varieties to Xanthomonas oryzae. Plant Disease Reporter 57:537-541.
14 Karganilla A, Paris-Natural M, Ou SH. 1973. A comparative study of culture media for Xanthomonas oryzae. Philipp Agric 57:141-152.
15 Lee BM, Park YJ, Park DS, Kang HW, Kim JG, Song ES, Park IC, Yoon UH, Hahn JH, Koo BS, Lee GB, Kim H, Park HS, Yoon KO, Kim JH, Cho HJ, Koh NH, Seo JS, Go SJ. 2005. The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice. Nucleic Acids Research 33:577-586.   DOI
16 Li ZK, Arif M, Zhong DB, Fu BY, Xu JL, Domingo- Rey J, Ali J, Vijayakumar CHM, Yu SB, Khush GS. 2006. Complex genetic networks underlying the defensive system of rice(Oryza sativa L.) to Xanthomonas oryzae pv. oryzae PNAS. 103 (21):7994-7999.   DOI   ScienceOn
17 Mew TW, Alvarez AM, Leach JE, Swlngs J. 1993. Focus on Bacterial Blight of Rice. Plant Disease 77:5-12.   DOI
18 Mundt CC, Nieva LP, Vera Cruz CM. 2002. Variation for aggressiveness within and between lineages of Xanthomonas oryzae pv. oryzae. Plant Pathology 51:163-168.   DOI   ScienceOn
19 Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473-497.   DOI
20 Nakashima K, Tran LS, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K. 2007. Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice. Plant J 51:617-630   DOI   ScienceOn
21 Olsen AN, Ernst HA, Leggio LL, Skriver K. 2005. NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci 10:79-87.
22 Ramesh S, Nagadhara D, Reddy VD, Rao KV. 2004. Production of transgenic indica rice resistant to yellow stem borer and sap-sucking insects, using super-binary vectors of Agrobacterium tumefaciens. Plant Sci 166: 1077-1085.   DOI   ScienceOn
23 Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, New York, NY: Cold Spring Harbor Laboratory.
24 Sasaki T, Matsumoto T, Antonio BA, Nagamura Y. 2005. From mapping to sequencing, post-sequencing and beyond. Plant Cell Physiol 46:3-13.   DOI   ScienceOn
25 Shin MS, Kim KY, Park HS, Ko JK. 2011. Breeding for resistance to bacterial blight in rice. Kor. J. Breed. Sci. 43(4):251-261.
26 Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2004. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481-2498.   DOI   ScienceOn
27 Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J. 2006. A NAC Gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314: 1298-1301.   DOI
28 Wu XM, Li YR, Zou LF, Chen GY. 2007. Gene-for-gene relationships between rice and diverse avrBs3/pthA avirulence genes in Xanthomonas oryzae pv. oryzae. Plant pathology 56:26-34.
29 Xie Q, Frugis G, Colgan D, Chua NH. 2000. Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development. Genes Dev 14:3024- 3036.   DOI   ScienceOn