This paper aims to investigate the effect of anticipatory motion in Wall E (2008). For the purpose of the study, first of all, an overall discussion on the definition and role of anticipation presented through the related literature review. Based on this, the present study analyzed the functions of anticipatory movements of the main characters, Wall E and EVE in 29 scenes. As a result, this paper found that anticipation is involved in expressions of humanity, thought process, utterance and intonation, the degree of emotion and energy, reinforcement of the character's intention, and biological response within the film. Overall, these findings show that anticipation takes an important part in giving the illusion of life to the characters throughout the movie, which may imply that anticipation can be beneficial for characters with abstract form or limited modality. The ultimate goal of this study is to propose that the animation principles can be used as a tool to analyze character animation and can be applied academically or industrially to other fields such as artificial intelligence.