The present work analyzed the effect of mixing vane shape on the flow structure and heat transfer downstream of mixing vane in a subchannel of fuel assembly, by obtaining velocity and pressure fields, turbulent intensity, flow-mixing factors, heat transfer coefficient and friction factor using three-dimensional RANS analysis. NJl5, NJ25, NJ35, NJ45, which were designed by the authors, were tested to evaluate the performances in enhancing the heat transfer. Standard $\kappa-\epsilon$ model is used as a turbulence closure model, and, periodic and symmetry conditions are set as boundary conditions. The flow blockage ratio is kept constant, but the twist angle of mixing vane is changed. The results with three turbulence models( $\kappa-\epsilon$, $\kappa-\omega$, RSM) were compared with experimental data.