This paper presents the optimal shape of microelectrode that generates dielectrophoretic(DEP) force to separate particles in homogeneous medium. The principle of the particles sorting is based on the use of the relative strengths of negative DEP (nDEP) and drag forces, as in a general DEP-activated cell sorter (DACS). To numerically calculate the DEP force and drag force, the simulation is implemented in MATLAB 7.0. The properties of particles, which are used in simulation, are similarly selected as those of cells to apply cell separation. The most optimized shape of electrode is selected by numerical simulation according to a variety of electrode shape such as rectangle, trapezoidal, and right-triangle. Through, in addition, parameter study, we found that applied frequency is more significant factor on the separation than various parameters, such as applied voltage and permittivity of medium, that decide on the strength of DEP force.