Browse > Article
http://dx.doi.org/10.3795/KSME-A.2009.33.1.49

The Electrode Shape for the Efficient Separation of Cell in Dielectrophoresis-Activated Cell Sorter  

An, Jae-Min (한국한공대학교 항공우주 및 기계공학부)
Chae, Seung-Yeub (한국한공대학교 항공우주 및 기계공학부)
Park, Seok-Ho (전남대학교 기계시스템공학부)
Kim, Byung-Kyu (한국항공대학교, 항공우주 및 기계공학부)
Publication Information
Transactions of the Korean Society of Mechanical Engineers A / v.33, no.1, 2009 , pp. 49-55 More about this Journal
Abstract
This paper presents the optimal shape of microelectrode that generates dielectrophoretic(DEP) force to separate particles in homogeneous medium. The principle of the particles sorting is based on the use of the relative strengths of negative DEP (nDEP) and drag forces, as in a general DEP-activated cell sorter (DACS). To numerically calculate the DEP force and drag force, the simulation is implemented in MATLAB 7.0. The properties of particles, which are used in simulation, are similarly selected as those of cells to apply cell separation. The most optimized shape of electrode is selected by numerical simulation according to a variety of electrode shape such as rectangle, trapezoidal, and right-triangle. Through, in addition, parameter study, we found that applied frequency is more significant factor on the separation than various parameters, such as applied voltage and permittivity of medium, that decide on the strength of DEP force.
Keywords
Conductivity; Dielectrophoresis; Electrode; Permittivity; Separation;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Malyan, B., and Balachandran, W., 2001, "Sub-Micron Sized Biological Particle Manipulation and Characterization," Journal of Electrostatics, Vol. 51-52, pp. 15-19   DOI   ScienceOn
2 Durr, M. et al., 24 Feb 2003, "Microdevices for Manipulation and Accumulation of Micro- and Nanoparticles by Dielectrophoresis," Electrophoresis, Vol. 24, Issue 4, pp. 722-31   DOI   ScienceOn
3 Pohl, H., 1978, "Dielectrophoresis," Cambridge University Press, New York
4 Pethig, R., 1979, "Dielectric and Electronic Properties of Biological Materials," JohnWiley & Sons, Chichester, New York, Brisbane, Toronto
5 Jones, T.B, 1995, "Electromechanics of Particles," Cambridge University Press, Cambridge, New York, Melbourne
6 Pethig R., 1979, "Dielectric and Electronic Properties of Biological Materials," JohnWiley & Sons, Chichester
7 Schnelle, T. et al., March 1999, "The Influence of Higher Moments on Particle Behaviour in Dielectrophoretic Field Cages," Journal of Electrostatic, Vol. 46, pp. 13-28   DOI   ScienceOn
8 Coley, H. M., Labeed, F. H., Thomas, H. and Hughes, M. P., 2007 "Biophysical Characterization of MDR Breast Cancer Cell Lines Reveals the Cytoplasm is Critical in Determining Drug Sensitivity," Biochimica Et Biophysica Acta-General Subjects, Vol. 1770, pp. 601-608   DOI   ScienceOn
9 Park, J., 2005, "Design and Analysis of MEMS-based Cell Manipulation System for Cellomics," Seoul National University, Submitted in Partial Fulfillment of the Requirements for the Degree of Ph. D, pp. 46-48