• 한국지능시스템학회논문지
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• 제20권1호
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• pp.42-47
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• 2010

마이크로 마우스를 이용한 다수의 미로 경진대회가 개최되어 미로 탐색 알고리즘의 성능이 비교되고 있으며, 미로 탐색 알고리즘은 좌(우)수법, 구심법, 언덕오르기 등을 기본으로 하여 다양한 형태로 적용되어 사용되고 있다. 하지만 미로 탐색알고리즘을 적용하여 테스트하기 위한 소프트웨어 플랫폼이 없어서 프로그램을 직접 개발하거나 하드웨어를 통해 알고리즘의 성능을 테스트해야 하는 불편함을 겪는다. 본 연구에서는 하드웨어로 구현이 어려운 다양한 형태의 미로 제작과 알고리즘의 손쉬운 적용이 가능하고, 스텝, 연산 횟수, 탐색 시간의 평가가 가능한 미로 탐색 알고리즘을 위한 플랫폼을 개발하였다. 플랫폼은 메인 레이어, 인터페이스 레이어, 사용자 레이어의 분리 구조로 되어 알고리즘을 쉽게 교체적용 할 수 있는 장점이 있다. 플랫폼의 실험을 통하여 미로 탐색 알고리즘들의 성능을 평가하고 분석하여 알고리즘의 개발 및 실험에도 적용할 수 있음을 확인하였다.

• 대한한방소아과학회지
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• 제18권1호
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• pp.207-220
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• 2004

Objective: This study was conducted to find out the effect of saruktanggamibang(SRT) on memory and learning of rats. Methods: The mon·is water maze was used. It was performed Acquisition test, Retention test of water maze. After Behavioral test, it was investigated AChE cell numbers of CAI and CA3 on hippocampus. Results: of water maze revealed that acqusitive abilities of SAP+SRT group significantly improved on 3, 4, 5th day compared with SAP+Saline group. Retention test of water maze didn't reveal that retentive abilities of SAP+SRT group significantly improved comparing with another group. ChAT cell numbers of medial septum of SAP+SRT group significantly increased compared with SAP+Saline group. AChE cell numbers of CAl and CA3 on hippocampus of Sham+SRT group significantly increased compared with Sham group. And AChE cell numbers of CAl and CA3 on hippocampus of SAP+SRT group significantly increased compared with SAP+Saline group. Conclusion: Saruktanggamibang(SRT) has an effect on memory and learning of rats.

• 동의신경정신과학회지
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• 제19권1호
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• pp.29-42
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• 2008

Objectives : This study was performed to examine the effect of Sinchim on enhancing of memory in rat with water maze. Methods : Experimental animals(white rat) were classfied into normal, control, and aroma sample group. Then, they were injected .{\beta}-amyloid into control and aroma sample group rat's brain about $5{\mu}l$ to injure its brain. After rat smelt Sinchim about 12days, I did water maze test and anatomized its hippocampus. Sections were cut coronally at 30 ${\mu}m$.(XI00) Results: 1. In acquisition test of water maze learning, .{\beta}-amyloid injured group took more time than normal group to reach the escape platform noticeably and through the session of trial, Sinchim aroma sample group shortened time than .l3-amyloid injured group after 6 days. 2. In the acetyltransferase(AchE) immunostained method, it was shown that Sinchim aroma sample recovered tbe syntbesis of ChAT(Choloneacetyltransferase). Conclusion: Smelling Sinchim would be useful for enhancing of memory.

• Biomolecules & Therapeutics
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• 제22권5호
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• pp.467-473
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• 2014

The purpose of this study is to investigate the memory enhancing effect and underlying molecular mechanism of arabinoxylan (AX), a major component of dietary fiber in wheat against scopolamine (SCO)-induced amnesia in Sprague-Dawley (SD) rats. Diverse behavior tests including Y-maze, Morris water maze, and passive avoidance tests were performed to measure cognitive functions. SCO significantly decreased the spontaneous alterations in Y-maze test and step-through latency in passive avoidance test, whereas increased time spent to find the hidden platform in Morris water maze test compared with the sham control group. In contrast, oral administration of AX (25 mg/kg and 50 mg/kg) effectively reversed the SCO-induced cognitive impairments in SD rats. Furthermore, AX treatment up-regulated the expression of brain-derived neurotrophic factor (BDNF) in the cortex and hippocampus via promoting activation of cAMP response element binding protein (CREB). Therefore, our findings suggest that AX can improve SCO-induced learning and memory impairment possibly through activation of CREB and up-regulation of BDNF levels, thereby exhibiting a cognition-enhancing potential.

• Animal cells and systems
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• 제6권4호
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• pp.287-292
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• 2002

Functional characterization of sex pheromones in olfactory responses is essential for the study of chemical communications in amphibians. Using Y-maze olfactory preference tests, we have investigated the olfactory res-ponses of male and female red-spotted newts, Notophthalmus viridescens, to the crude extracts of the opposite sex's genial and cloacal pheromones. Both male and female cloacal pheromone extracts caused the opposite sex to initiate olfactory responses by leaving the starting area in the Y-maze, but only subject males exposed to female cloacal pheromone extracts completed olfactory choice by entering the side arm of the Y-maze which received the pheromones. For genial pheromone extracts, only female genial pheromone extracts induced initial olfactory responses from test males. Neither male nor female genial pheromone extracts made the oppo-site sex complete olfactory choice. Pre-exposure of test females to male pheromone extracts increased the likelihood of initial olfactory responses. The latency for initial olfactory responses of test females that were previously exposed to male genial extracts was significantly shorter than that of control females.

• 동의신경정신과학회지
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• 제10권1호
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• pp.1-15
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• 1999

The effects of Sahyangsohapwon on the enhancement of learning and memory of AD model rats were studied with Morris water maze and radial arm maze. Sample group was electrolytically lesioned on nbM, and then daily treated with the medicine for two months. Control group with nbM lesion, and sham group with the sham operation were treated the vehicle for same duration. The following results were observed. In the learning trials of Morris water maze, all three groups were improved in learning capacity as trials were repeated, but the sham group showed more prominent improvement in learning compared with the control group(p<0.01). 2. In memory retention test of Morris water maze, the sham group marked more significant improvement statistically in memory retention compared with the control group(p<0.05). 3. In the learning of radial arm maze, the sham group shows better learning capacity significantly compared with the control group(p<0.05). With the experimental results above, Sahyangsohapwon can be supposed to have the improving effects on the learning and memory of AD rats induced by electronical injury of nbM.

• 동의신경정신과학회지
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• 제21권2호
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• pp.61-73
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• 2010

Objectives : The purpose of this study was to assess anti-depressive effects of Bee Venom(BV) on an Animal Model of Depression induced immobility stress. Methods : There was 2 pre-experiments MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Western blot test and 3 main experiments ; forced swimming test, tail suspension test and Y-maze task. Male rats were used for main experiment. The subject was divided into 4 groups(1. control group injected only saline, without immobility stress 2. Negative group injected saline after 2 hours immobility stress 3. Positive group injected Amitriptyline after 2 hours immobility stress 4. BV group injected Bee Venom after 2 hours immobility stress). Each group consisted of 6 rats. Forced swimming test, tail suspension test, Y-maze task were used to evaluate anti-depressive effect of Bee Venom. Results : In MTT assay, as the density of BV increased, the existence rate of primary neuronal cell increased. In Western blot test, the density of CREB and AKT was increasing as time went by. In forced swimming test, BV group showed immobility decreased more than Normal group and Positive group. In tail suspension test, Normal group and Positive group showed immobility decreased more than BV group. In Y-maze task, BV group showed immobility decreased more than Normal group, but Positive group showed immobility decreased more than BV group. Conclusions : These results suggest that Bee Venom may have anti-depressive effect on depression.

• 한국식품저장유통학회지
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• 제19권5호
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• pp.767-773
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• 2012

괴각 추출물의 항불안 효능을 탐색하기 위하여 elevated plus-maze, horizontal wire test 및 open field test와 같은 동물행동실험을 통하여 비교한 결과 다음과 같은 결론을 얻었다. Elevated plus-maze를 이용한 본 연구에서 괴각 추출물의 100 mg/kg, 200 mg/kg 및 400 mg/kg 투여군에서 대조군에 비하여 open arm에서 머무른 시간의 백분율이 증가하였고, open arm으로의 출입 백분율 또한 증가하였다. Elevated plus-maze를 이용한 길항실험에서는 benzodiazepine 수용체의 antagonist인 flumazenil에 의해 괴각 추출물 400 mg/kg의 항불안 효능이 차단되는 것이 관찰되었다. Locomotor activity 측정에서도 괴각 추출물의 모든 용량에서 총 이동거리의 변화가 없었으며, 또한 horizontal wire test에서도 대조군과 괴각 추출물 투여군 사이에 차이를 나타내지 않았다. 결론적으로, 본 연구의 결과에서 괴각의 메탄올 추출물이 elevated plus-maze test, horizontal wire test 및 open field test를 통하여 locomotor activity 및 근육이완이나 진정 등의 부작용이 없으면서 우수한 항불안 작용을 가지는 천연물이라고 생각되어지며 이러한 작용이 특히 GABA 신경계와 관련이 있음을 시사하고 있다. 향후 괴각의 항불안 작용의 평가를 위하여 다양한 실험모델의 개발이 필요할 것으로 생각되며 또한 이러한 작용에 대한 기전 연구가 포괄적이고 다각적으로 진행되어져야 할 필요성이 있다고 사료된다.

• Journal of Ginseng Research
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• 제32권4호
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• pp.341-346
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• 2008

백삼과 홍삼추출물 투여가 scopolamine으로 기억을 손상시킨 실험동물의 학습수행 개선과 뇌조직의 AChE 억제 및 ACh 함량을 평가하여 다음과 같은 결론을 얻었다. 1. 백삼과 홍삼추출물(200 mg/kg, p.o.) 투여는 scopolamine (2 mg/kg, i.p.)으로 유발된 기억손상 동물모델의 Morris water maze 시험에서 tacrine 투여군과 대조군처럼 인지훈련이 반복될수록 escape latency 시간이 현저히 감소하였다. probe test에서도 scopolamine 투여군보다 유의적으로 감소하여 인삼추출물이 장기기억(Long-term memory)과 working memory의 개선에 효과가 있음을 확인하였다. 2. Y-maze test를 통한 실험동물의 자발적 변동행동력은 실험군간 차이가 없었으며, 총 출입회수도 차이가 없어 인삼추출물은 실험동물의 일반적 운동성에 영향을 주지 않았다. 3. 해마조직의 AChE 활성은 인삼추출물 군에서 감소하는 경향을 보였으나 유의적 차이는 없었다. 대뇌피질과 혈청 AChE 활성은 홍삼추출물 투여군에서 유의적으로 감소하였다. 4. 인삼추출물 투여군의 뇌조직 ACh 함량은 대뇌피질과 해마조직에서 scopolamine 투여군보다 증가하는 경향을 보였으나 그룹간의 유의적인 차이는 없었다. 5. 이를 통하여 볼 때 인삼추출물 특히 홍삼추출물은 장기기억 활성촉진, AChE 억제, ACh 활성촉진으로 콜린성 신경계를 자극하여 기억 및 학습증진에 매우 효과적으로 작용하는 항치매 물질로 사료된다.

• 동의신경정신과학회지
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• 제21권1호
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• pp.125-144
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• 2010

Objectives: The purpose of this study was to characterize the effect of the fraction of Korean mountain ginseng folium (FKG) on the learning and memory impairments induced by scopolamine. Methods: The memory ameliorating effect of FKG was investigated using a passive avoidance test, the Y-maze test, and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine(1mg/kg, i.p.). Results: FKG (2 or 4mg/kg, p.o.) administration significantly reversed scopolamine-induced cognitive impairments in mice by the passive avoidance test and the Y-maze test(P<0.05), and also improved escape latency in the Morris water maze test at 2 or 4mg/kg(P<0.05). Although FKG has little inhibitory activity for AChE (IC50 value; 1847 ${\mu}g/ml$) in an invitro study, phosphorylated extracellular signal-regulated kinase(pERK) was increased by the administration of FKG inhippocampus on immunohistochemistry. Conclusions: These results suggest that FKG may be a useful cognitive impairment treatment, and its beneficial effects are mediated, in part, via activation of ERK pathway.