• Korean Journal of Clinical Laboratory Science
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• v.45 no.1
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• pp.1-4
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• 2013

Vancomycin-resistant enterococci (VRE) have emerged as important healthcare-associated infection since last two decades. ChromID VRE agar (cIDVA) is useful for VRE rectal swab screening. We investigated all VRE were isolated on the cIDVA. A total of 363 rectal swabs of 85 patients to test VRE screening were inoculated into bile-esculin (B-E) broth with $6{\mu}g/mL$ vancomycin. After 24 hours incubation, we subcultured B-E broths were changed to black onto cIDVA. All isolates were identified by the MICROSCAN and VITEK2. The vanA gene and vancomycin minimal inhibition concentration (MIC) were detected by PCR and E-test respectively. 277 E. faecium (84.7%), 16 E. faecalis (4.9%), 25 E. avium (7.6%), 8 E. gallinarum (2.4%) and 1 E. raffinosus (0.3%) were isolated. 10.3% of VRE detected on cIDVA were other than E. faecium and E. faecalis that presented various color from colorless to pale violet. All isolates contained vanA and vancomycin MIC were > $256{\mu}g/mL$. VRE isolates other than E. faecium and E. faecalis should be objective to the contact precautions for healthcare-associated infection control if they possess vanA gene. Due to emerging enterococci carrying vanA such as E. avium, E. gallinarum, and E. raffinosus, VRE surveillance should be expanded to all isolates on chromogenic agar.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.10b
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• pp.63-64
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• 2002

In a micro-scale contact, surface forces such as capillary force and van der Waals Interaction significantly Influence the contact between asperities of rough surfaces. Little is, however, known about the variation of these surface forces as a function of chemical property of the surface (hydrophilicity), relative humidity and deformation of asperities In the real area of contact. A better understanding of these surface forces is of great necessity in order to find an optimal solution for reducing friction and adhesion of micro surfaces. We proposed an effective method to analyze capillary and van der Waals forces In nano-scale contact. In this method, Winklerian foundation model was employed to analyze the contact of rough surfaces that were obtained from atomic force microscopy (AFM) height Images. Self-mated contact of diamond-like-carbon (DLC) coatings was analyzed, as an example, by the proposed model. It was shown that the capillary force was significantly influenced by relative humidify and wet angle of the DLC surface. The deformation of asperities to a critical magnitude by external loading led to a considerable increase of both capillary and van der Waals forces.

• Korean Journal of Microbiology
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• v.48 no.2
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• pp.116-124
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• 2012

Identification was performed in March 2008 for the 76 Enterococcus strains isolated from the Han-river, which is used as water supply for Seoul citizens. The antibiotic susceptibility, antibiotic resistant structural analysis, trans-conjugation, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were also carried out for the isolated strains. Among the isolated strains, 25 strains were E. casseliflavus, 4 strains were E. faecalis and 1 strain was E. hirae. Investigation of antibiotic susceptibility indicated that 15 strains demonstrated tolerance against vancomycin, and that 11 strains of E. faecium and 4 strains of E. casseliflavus were VRE. The vanA gene detection of the VRE strains revealed that 6 E. faecium strains were vancomycin-resistant Enterococcus faecium (VREF) possessing vanA. Analyses of transposon Tn1546 structure containing vanA demonstrated that Km36 and Km37 belonged to Tn1 type, Km20 and Km38 was Tn2 type, and Km39 and Km40 was Tn3 type. PFGE disclosed that among the 6 VREF strains, Km36 and Km37 exhibited equivalent subtype, while the rest 4 strains showed subtypes different to each other. MLST for the 6 VREF strains disclosed that 3 strains were ST78, while the rest 3 strains were ST18, ST192 and ST230, respectively. All these clonal complexes were derived from CC17 which has been isolated from clinical sources. 4 strains belonged to CC78, while the rest 2 strains were CC18 and CC192, respectively.

• Korean Journal of Veterinary Research
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• v.39 no.2
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• pp.343-352
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• 1999

Vancomycin resistant enterococci (VRE) have emerged as an important nosocomial pathogen. Since 1989 the Center for Disease Control, United States, has reported a rapid increase in the incidence of enterococcal bacteremia and endocarditis infection by VRE. It was suggested that the use of avoparcin was associated with the appearance of VRE in animal husbandry. To date, several detection methods have been used based on conventional methods of culture and gene detection. However, these methods have some limitations such as time-consuming, laborious and additional differential needs. Therefore, In this study a multiplex PCR method was established to detect and differentiate resistance types of enterococci which specifically amplify the four van genes encoding vancomycin resistance elements. Using the method, we investigated the incidence rates and types of VRE from farms using or not using avoparcin. A total of 1091 animal fecal samples were collected from 70 pig and 32 poultry farms. A total of 425 of enterococci were isolated from samples. Of the 425 isolates, 11 of the them showed a pattern of high-level vancomycin resistance (MIC : $64{\sim}256{\mu}g/ml$) which was associated with the presence of the vanA or vanB gene. Fifty-seven isolates showed a pattern of low-level vancomycin resistance (MIC : $3{\sim}8{\mu}g/ml$) associated with the vanC-1 or vanC-2 gene. Interestingly, all isolates with high-level vancomycin resistance were from farms that have never used avoparcin. Moreover, the high-level VRE isolation rate in Korea (2.58%) was much lower than that of other countries (50% in England, 7% in Belgium) where avoparcin have been used. In conclusion, the multiplex PCR method established in this study could be applied for detection of VRE.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.11
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• pp.1617-1620
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• 2013

The widely-used measurement methods for conductivity of non-magnetic metals are van der Pauw method, Two Point Probe method and Eddy Current method. Among them a more simpler and easier method is the Eddy Current method and an instrument using the method is a Conductivity Meter which can measure a conductivity by contacting its probe on a sample surface. However, conductivity standards are essentially needed to confirm the meter's performance or to calibrate it. In this study, six kinds of the standards which are made of Cu, Al-1, Al-2, brass, Zn and SUS-316 are developed and conductivity ranges for the standards are 2.27 %IACS ~ 101.6 %IACS with measurement uncertainty of less than 0.3 %.

• Korean Institute of Interior Design Journal
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• v.17 no.1
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• pp.19-29
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• 2008

The purpose of this study is to reveal that the concept of relation in the postwar CIAM's debate was the hot issue which leaded to the collapse of CIAM, and that Aldo van Eyck developed it into the concept of "in-between". The postwar CIAM's young members voiced a critique of the universalizing attitude toward dwelling that CIAM had pursued since 1928 and looked for a new approach that would take into account the relation around dwelling. So, this study reviews the concepts of relation which were proposed by the postwar CIAM's young members. As a result, it is revealed that Aldo van Eyck extended the concept of "in-between" not to have the polarities, such as inside/outside, close/open, part/whole, neutralize or cancel one another out, but to attune them in such a way that they reinforce each other by mutual contrast, while the other young CIAM's members limited it to transition or connection. And, this paper analyzes the Municipal Orphanage in Amsterdam which was presented in CIAM 10. Through the analysis, it is revealed that the building presents many polarities simultaneously such as those; indoor and out door realms, dynamics and immutability, part and whole, and so on. In conclusion, it is possible to recognize that the concept of "in-between" is the fundamental binary-compound relation that lies at the root of Aldo van Eyck's architectural thought and bears on an interaction on a reciprocal relation between the inhabitant and the built environment.

• Communications of Mathematical Education
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• v.30 no.4
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• pp.435-452
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• 2016

This study was designed to explore students' instrumentalization in relation to the van Hiele's teaching method within a technology environment using GeoGebra. To carry out the study, a total of 4 lesson units was developed based on van Hiele teaching method for two slow learners in Gyeonggi province, Korea. The results of study were as follows. Instrumentalization of students was actualized from preparation, to adaptation, and to application stages. In preparation, and adaptation stages, depending on visualization, students used a trial-and-error method a lot, however in application stage the role of GeoGebra was just to check the solution of what they conjectured. Therefore, a teacher should prepare geometric tasks according to the processes of instrumentalization based on geometric teaching method. During instrumentalization and instrumentation of users, usage scheme(US) and instrumented action scheme(IAS) should be concrete.

• Education of Primary School Mathematics
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• v.13 no.2
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• pp.85-97
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• 2010

The purpose of this study is to develop a teaching program in consideration of the geometrical thinking levels of students to make a contribution to teaching figures effectively. To do this, we checked the geometrical thinking levels of fourth-graders, developed a teaching program based on van Hiele's theory, and investigated its effect on their geometrical thinking levels. The teaching program based on van Hiele's theory put emphasis on group member interaction and specific activities through offering various geometrical experiences. It contributed to actualizing activity-centered, student-oriented, inquiry-oriented and inductive instruction instead of sticking to expository, teacher-led and deductive instruction. And it consequently served to improving their geometrical thinking levels, even though some students didn't show any improvement and one student was rather degraded in that regard - but in the former case they made partial progress though there was little marked improvement, and in the latter case she needs to be considered in relation to her affective aspects above all. The findings of the study suggest that individual variances in thinking level should be recognized by teachers. Students who are at a lower level should be given easier tasks, and more challenging tasks should be assigned to those who are at an intermediate level in order for them to have a positive self-concept about mathematics learning and ultimately to foster their thinking levels.

• Korean Institute of Interior Design Journal
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• v.14 no.1
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• pp.134-141
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• 2005

Ludwig Mies van der Rohe, one of the influential modernist architects in early 20th century, had an great effort to found the modern architecture through various kinds of works. Conceptually he tried to develop the trans-temporal architecture language by involving a reintegration of countermanding principles in both ideas and practices for designing buildings. The model for his striving to find such a synthesis or a dynamic form was the neo-classicism of K. F Schinkel, inasmuch as Schinkel endeavored to combine the classical rationalistic order with the creative act of construction and to make these two melt into his age. In the same horizon with that Mies van der Rohe attempted to express the industrialized civilization of his time as a given fact, though this phenomenal substance was the only authentic form that has been conceivable to the people. For him the modern technology was regarded as the manifestation of rationalistic transcendency, which can be interpreted always consequentially. Here In this context this study is purposed to consider through the analysis of the ground ideas of architecture and the designed chairs, how commensurable the tectonic value (in the architecture) that is constantly evident in his works and the various autonomous drives toward deformalization (in the furniture design) that is concerned with his interest for cultural situation and materiality of form are with each other. And then it will be tried to prove, that on the ground of the trans-temporal achitecture language the furniture design of Mies van der Rohe, which emphasizes curves as individual forms, could correspond to his architecture that lays priority to space and construction

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.13 no.4
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• pp.319-326
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• 2001

This paper reports on a recent investigation to determine criteria for the design of rubble-mound breakwaters. Existing theories and empirica] equations have been carefully reviewed and a new relation is proposed for the determination of optimum weight of armor unit of rubble-mound breakwater. A new parameter is introduced into the new semi-theoretical equation, which is closely related with the surface particle velocity of wave motion. The laboratory data reported by van der Meer(1987) were used for the determination of proper relations of empiricat parameters introduced into the new empirical equation.