• Title/Summary/Keyword: total phenolic and flavonoid content

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Antioxidative and Antibrowning Effects of Taraxacum platycarpum and Chrysanthemum indicum Extracts as Natural Antibrowning Agents (천연 갈변저해제로서의 민들레 및 감국 추출물의 항산화 및 갈변억제 효과)

  • Chang, Min-Sun;Park, Mi-Ji;Jeong, Moon-Cheol;Kim, Dong-Man;Kim, Gun-Hee
    • Food Science and Preservation
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    • v.18 no.4
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    • pp.584-589
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    • 2011
  • This study was conducted to investigate the effects of Taraxacum platycarpum and Chrysanthemum indicum extracts on the browning of apple slices. The inhibition of the PPO activities, total phenolic contents, and DPPH free-radical-scavenging activities was measured from water and from the 80% EtOH extracts of Taraxacum platycarpum and Chrysanthemum indicum as natural antibrowning agents. Apples were cut into 15-mm-thick slices, and each slice was dipped for 1 min in different treatment solutions (1% Taraxacum platycarpum and Chrysanthemum indicum extracts, 1% citric acid) and was stored at room temperature. The inhibition of the PPO activity in the Chrysanthemumin indicum extract was better than that in the Taraxacum platycarpum extract. The highest DPPH free-radical-scavenging activity (76.10%) was found in the 80% EtOH extract of Chrysanthemum indicum. After 24 h, the ${\Delta}E$ value of the apple slices that were treated in the 80% EtOH extract of Chrysanthemum indicum was at the lowest level (2.22). The total phenolic content was 94.07 mg/g, and the total flavonoid content was 102.60 mg/g, in the 80% EtOH extract of Chrysanthemum indicum. The antibrowning effect of Chrysanthemum indicum was higher than that of Taraxacum platycarpum in the apple slices.

Effects of the Drainage Methods on Phenolic Compounds and Radical Scavenging Activity of Foxtail Millet and Proso Millet (배수방법이 조와 기장의 페놀성분 및 라디칼 소거활성에 미치는 영향)

  • Jung, Ki Yuol;Ko, Jee Yeon;Lee, Jae Saeng;Jeong, Mi Sun;Oh, In Seok;Woo, Koan Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.282-292
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    • 2014
  • This study investigated the changes of antioxidant compounds and antioxidant activity of foxtail millet (FM) and proso millet (PM) by drainage methods in poorly drained sloping paddy field. The experimented soils in this study were very poorly drained (VPDP) and imperfectly drained paddy soil (IDP). Two drainage methods namely under pipe drainage (UPD) and open ditch (ODD) were installed within 1-m position at the lower edge of the upper paddy fields. The soil moisture contents of UPD and ODD on VPDP were $18.52{\pm}4.58$ and $19.01{\pm}4.25%$, and IDP were $14.87{\pm}4.82$ and $18.64{\pm}3.85%$, respectively. Generally, crop yields, proximate and minerals composition of FM and PM showed significant differences by drainage methods. The total polyphenol, flavonoid and tannin contents, and radical scavenging activity of the ethanolic extracts of FM and PM showed significant differences by drainage methods. The total polyphenol content of FM and PM by drainage methods was 1.69~2.30 and 1.18~1.35 mg GAE/g, total flavonoid content was 0.31~0.76 and 0.27~0.41 mg CE/g, and total tannin content was 0.36~0.54 and 0.21~0.28 mg TAE/g, respectively. The DPPH radical scavenging activity of FM and PM was 39.53~59.81 and 27.91~40.25 mg TE/100 g, and ABTS radical scavenging activity was 113.59~152.10 and 61.38~79.19 mg TE/100 g, respectively. The antioxidant compounds and radical scavenging activity of FM and PM by drainage methods were frequently affected soil water.

Optimization of Microwave Extraction Conditions for Antioxidant Phenolic Compounds from Ligustrum lucidum Aiton Using Response Surface Methodology (반응표면분석법을 이용한 여정자의 페놀계 항산화 성분에 대한 마이크로웨이브 추출조건 최적화)

  • Yun, Sat-Byul;Lee, Yuri;Lee, Nam Keun;Jeong, Eung-Jeong;Jeong, Yong-Seob
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.570-576
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    • 2014
  • Response surface methodology (RSM) was applied to optimize the microwave-assisted extraction (MAE) conditions for electron-donating ability, total phenol content, and total flavonoid content of Ligustrum lucidum Aiton. Ligustrum lucidum Aiton from different regions was tested, and Ligustrum lucidum Aiton from Haenam was chosen due to its higher total phenolic content, total flavonoid content, DPPH radical scavenging activity and ABTS radical scavenging activity compared to the other samples. Central composite design was used to optimize extraction of Ligustrum lucidum Aiton from Haenam as well as determine the effects of extraction temperature ($X_1$) and extraction time ($X_2$) on dependent variables ($Y_n$). Determination coefficients ($R^2$) of the regression equations for dependent variables ranged from 0.8858 to 0.9517. The optimum points were $131.68^{\circ}C$ for extraction temperature and 5.49 min for extraction time. Predicted values of the optimized conditions were acceptable when compared to experimental values.

Antioxidant Properties and Quality Characteristics of Dasik Supplemented with Longanae Arillus (용안육 다식의 항산화 활성 및 품질 특성)

  • Yang, Eun Young;Han, Young Sil;Sim, Ki Hyeon
    • The Korean Journal of Food And Nutrition
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    • v.31 no.4
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    • pp.485-494
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    • 2018
  • This study was designed to evaluate the quality characteristics and antioxidant properties of Longanae Arillus powder (added in the ratios of 0%, 25%, 50%, 75%, and 100%), which is traditionally used to render the product more suited to the modern consumer's taste for the compound Longanae Arillus Dasiks. As the consumer consumption of the proportions of Longanae Arillus increases, the moisture content and pH of the Dasik supplemented with Longanae Arillus decrease, while at the same time, a soluble solid content increases (p<0.001). The color value showed the decrease in L and b values with the increase in Longanae Arillus content, and the increase in the value of the compound with the addition of Longanae Arillus (p<0.001) can be noted. The mechanical texture of Dasik was increased by the addition of Longanae Arillus considering its hardness, adhesiveness, cohesiveness, springiness, and chewiness (p<0.001). In the sensory evaluation of the Longanae Arillus Dasik showed that people expressed an overall preference at the addition of Longanae Arillus, such as noted as being preferred with 50% in color, flavor, taste, texture, and overall acceptance of the compound (p<0.001). Regarding the antioxidant activity of Longanae Arillus Dasik, the total phenolic, flavonoid contents, DPPH radical scavenging activity, reducing power, and superoxide anion levels were found to increase with the addition of Longanae Arillus (p<0.001) with its consumer use. It is believed that Longanae Arillus Dasik, is most preferred to be added at the concentration of 50% during the Dasik preparation.

Antioxidant and Acetylcholinesterase Inhibitory Activities of Subcritical Water Extracts from Houttuynia cordata Thunb (어성초의 아임계수 추출물의 항산화 활성 및 Acetylcholinesterase 저해 활성)

  • Jo, Eun-Kyung;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.10
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    • pp.1391-1396
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    • 2011
  • Houttuynia cordata Thunb powder (0.05 g) was placed in a stainless vessel containing 10 mL of water, and subcritical water (SCW) extraction was carried out at various temperatures (50, 100, 200, and $300^{\circ}C$) for 10, 30, and 60 min. SCW treatment significantly affected physiologically important compounds, antioxidant activity, and acetylcholinesterase inhibitory activity of H. cordata Thunb extracts. H. cordata Thunb extracted at $300^{\circ}C$ for 30 min had the highest quercetin content, DPPH radical scavenging activity, total phenolic content, and acetylcholinesterase inhibitory activity, whereas the extract at $100^{\circ}C$ for 10 min possessed the highest flavonoid content. The highest ABTS radical scavenging activity was found in H. cordata Thunb extracted at 200 and $300^{\circ}C$ for 60 min. These results indicate that SCW extraction might be a useful processing method for obtaining valuable materials from H. cordata Thunb.

Effect of Various Pear Cultivars at Different Fruit Development Stages on Antioxidant and Whitening Activities (배 품종별 생육 단계에 따른 산화방지 및 미백 효과)

  • Yim, Sun-Hee;Cho, Kwang-Sik;Choi, Jin-Ho;Lee, Ju-Hyun;Kim, Myung-Su;Lee, ByulHaNa
    • Korean Journal of Food Science and Technology
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    • v.48 no.1
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    • pp.59-65
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    • 2016
  • This study was carried out to investigate the total polyphenols, antioxidant activities, and melanin synthesis inhibition of several pear cultivars (Pyruspyrifolia). The total polypenolic content of five pear cultivars was high in the unripe developmental stage. Total polyphenolic content of the Chuwhangbae cultivar extract was higher than that of other pear cultivars regardless of the developmental stage. However, the total flavonoid content did not differ between cultivars or developmental stages. The phenolic compound, arbutin has an inhibitory effect on melanogenesis. Arbutin levels in pear cultivars declined as the fruit matured. The free radical scavenging activity of the extract also decreased as the fruit ripened. In B16F10 mouse melanoma cells, most of the cultivar extracts inhibited melanin synthesis by about 50% at a $100{\mu}g{\cdot}mL^{-1}$ concentration, except in the Gamcheonbae extract until 90 days after full bloom. We have confirmed that the extract of pear cultivars have antioxidant activity and skin-whitening effects.

Investigation on Antibacterial and Antioxidant Activities, Phenolic and Flavonoid Contents of Some Thai Edible Plants as an Alternative for Antibiotics

  • Lee, J.H.;Cho, S.;Paik, H.D.;Choi, C.W.;Nam, K.T.;Hwang, S.G.;Kim, Soo-Ki
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.10
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    • pp.1461-1468
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    • 2014
  • This study was aimed to examine the antibacterial and antioxidative properties of seven edible plants from Thailand to develop alternative antibiotics as feed additives. The plants include Citrus aurantifolia Swingle (Lime) fruits and its leaves, Sesbania grandiflora L. (Agati sesbania) leaves, Piper sarmentosum Roxb (Wild betal) leaves, Curcuma domestica Valeton (Turmeric) roots, Morinda citrifolia L. (Beach mulberry) leaves, Cassia siamea britt (Siamea cassia) leaves, and Cocos nucifera L. (Coconut) peels. The plants were extracted by methanol, n-hexane, chloroform, ethyl acetate, butanol and water. Antibacterial activities with minimum inhibitory concentration (MIC) were determined by agar diffusion assay against Escherichia coli, Burkholderia sp., Haemopilus somnus, Haemopilus parasuis, and Clostridium perfringens that were considered pathogenic strains in livestock infection. Methanol extracts of C. aurantifolia Swingle fruits and leaves showed the broadest spectrum of antibacterial activities except for C. perfringens. Butanol extract of S. grandiflora L. leaves showed the strongest activity against Burkholderia sp. with MIC, $135{\mu}g/mL$. P. sarmentosum Roxb leaves showed antibacterial activities against E. coli, Burkholderia sp. and H. parasuis. Ethyl acetate and water extracts from C. domesitca Valeton roots showed MIC of $306{\mu}g/mL$ and $183{\mu}g/mL$, respectively against only C. perfringens. Antioxidative activity was determined by 2-diphenyl-2-picryl hydrazyl photometric assay. The methanol extracts of C. aurantifolia Swingle fruits and P. sarmentosum Roxb leaves showed the highest antioxidant activity among all the extracts with 3.46 mg/mL and 2.70 mg/mL effective concentration 50% ($EC_{50}$) values, respectively. Total contents of phenolics and flavonoids were measured from the plant extracts. Methanol extracts of S. grandiflora L. and chloroform extracts of C. domestica Valeton were found to have the highest amount of total phenolics, 41.7 and $47.8{\mu}g/mL$, respectively. Flavonoid content of methanol extracts in S. grandiflora L. T was $22.5{\mu}g/mL$ and the highest among plant extracts tested. These results indicated that C. aurantifolia Swingle, S. grandiflora L., P. sarmentosum Roxb, and C. domestica Valeton have antibacterial and antioxidant activities and can be used as alternative antibiotics or potential feed additives for the control of animal pathogenic bacteria.

Evaluation of Proanthocyanidin Contents in Total Polyphenolic Compounds of Pine (Pinus densiflora) Needle Extracts and Their Antioxidative Activities (적송잎 용매별 추출물의 총 폴리페놀성 성분에 대한 proanthocyanidin의 함량 분석 및 항산화 활성 검증)

  • Lee, Ok-Hee;Kim, Ki-Young;Jang, Min-Kyung;Yu, Ki-Hwan;Kim, Sung-Gu;Kim, Mi-Hyang;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.18 no.2
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    • pp.213-219
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    • 2008
  • Flavonoids in pine needles are known to be effective scavengers of free radical. Especially, proanthocyanidin, a kind of flavonoids possesses cardiovascular protection and antioxidative activities. Here, we evaluated proanthocyanidin contents in total polyphenolic compounds of pine needle extracts prepared by using hot water, ethanol, hexane or sub-supercritical $CO_2$. Analyses of total polyphenolic compounds and proanthocyanidin in each extracts indicated that hot water extract contained the highest concentrations, but sub-supercritical extract contained the lowest concentrations. On the other hand, evaluation of proanthocyanidin contents in total polyphenolic compounds in each extracts showed that sub-supercritical extract possessed the highest content, but hot water extract possessed the lowest content. These results indicate that extracts containing high concentrations of both total polyphenolic compounds and proanthocyanidin could be obtained by using hot water or ethanol extractions. Furthermore, extract containing high content of proanthocyanidin out of total polyphenolic compounds could be achieved by using sub-supercritical extraction. Measurement of antioxidative activities of extracts showed that hot water extract possessed the highest activity. In this study, we prepared extracts from pine needles by four different methods and evaluated the antioxidative compounds in extracts that could be used for effective components of functional food products.

Comparison of Plant Growth, Dormancy Breaking, Yield, and Biological Activities of Extracts in Four Asparagus Cultivars (아스파라거스 4 품종의 생장, 휴면타파, 수량 및 추출물의 생리활성 비교)

  • Lee, Jong Won;Heo, Buk Gu;Bae, Jong Hyang;Ku, Yang Gyu
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.796-804
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    • 2015
  • The present study investigated the plant growth characteristics, dormancy breaking, yield, and biological activity properties (contents of flavonoid and phenolic compounds, nitrite scavenging activity, and the survival rate of lung cancer) of four asparagus cultivars. It aimed to examine selection possibilities for high efficacy cultivar to promote biological activity in asparagus cultivation in South Korea. The results showed that the number of asparagus buds and root fresh and dry weight of 'Jersey Giant', 'Jersey Supreme', and 'NJ953' were higher than those of 'UC157' cultivar, and there were no differences in the number of roots. The dormancy breaking period of 'Jersey Supreme' was prolonged compared to the other cultivars. 'Jersey Giant' and 'NJ953' had more total spears, and greater spear diameter, and yield than other cultivars. Total flavonoid content was unaffected by cultivar and was higher in ethanol extraction than in hot water extraction. Total phenolic compound content was the lowest in 'NJ953' for both hot water and ethanol extracts, whereas in the ethanol extraction, 'UC157' had the highest, with $39.23mg{\cdot}L^{-1}$. 'Jersey Giant', 'Jersey Supreme', and 'NJ953' all showed greater than 70% nitrite scavenging activity. In the case of ethanol extraction, the survival rates of lung cancer in extracts from 'Jersey Giant', 'Jersey Supreme', and 'UC157' were lower than 'NJ 953' cultivar, regardless of extraction concentration. The survival rate of lung cancer was lower in hot water extraction than in ethanol extraction, so that the consumption of asparagus is also expected to be helpful in preventing lung cancer. The growth characteristics and biological activity effects of edible asparagus that were identified in the present study are expected to be useful in selection of high efficacy cultivars for biological activity and utilization.

Changes of Antioxidant Activity in Juglans mandshrica Maxim. Leaves by Far Infrared Ray Irradiation (원적외선 조사에 따른 가래나무 잎의 항산화 활성 변화)

  • Eom, Seok-Hyun;Park, Hyoung-Jae;Jin, Cheng-Wu;Park, Seung-Moon;Kim, Myong-Jo;Yu, Chang-Yeon;Cho, Dong-Ha
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.266-270
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    • 2007
  • This research was conducted to investigate antioxidant activities of Juglans mandshrica forages by the irradiation of far infrared ray during leaf drying processes before compound extraction, with its potential use for activating antioxidants. The contents of total phenolics and flavonoids in the basis of a gram leaf dry weight were maximized in 20 min at 30 V of far infrared irradiation. Total phenolic content was 25.43 mg/g in the FIR treatment, while it was 21.12 mg/g in the non-FIR treatment control. Total flavonoid content was 39.38 mg/g in the FIR treatment, while it was 32.78 mg/g in the non-FIR treatment control. The contents were decreased when the condition of far infrared ray irradiation was higher voltage and longer treatment time, exhibiting 16.93 mg/g of total phenolics and 23.78 mg/g of total flavonoids in 30 min at 50 V of far infrared irradiation. The antioxidant activities were shown to positive relationships with the contents of total phenolics and total flavonoids. In this study, we concluded that content of bioactive molecules relating antioxidant activity in Juglans mandshrica forages is optimized in 20 min at 30 V of far infrared irradiation for leaf drying process.