• Archives of Pharmacal Research
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• 제19권2호
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• pp.171-172
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• 1996

• 한국어병학회지
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• 제34권1호
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• pp.39-46
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• 2021

Tetracycline (TC)은 국내에서 세균 감염 치료에 반드시 필요한 대표적인 항생제로 알려져 있다. 그러나 부적절한 사용과 남용으로 인해 Tetracycline, Erythromycin, Fluoroquinolone 등과 같은 항생제에 대한 내성이 발생하고 있으며, 이는 국내 양식 분야에서 심각한 경제적 피해를 유발한다. 본 연구에서는 2015~2018년에 걸쳐 경기도 양식장의 양식생물에서 101개 균주를 분리하였다. 분리균주는 간이적인 생화학적 방법을 통해 동정하였으며, 최소 억제농도(MIC)의 확인을 통해 Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN)에 대한 내성 여부를 확인하였다. 이중 TC에 내성을 보이는 균주는 PCR법을 통해 tet 유전자의 분포를 조사하였다. 그 결과, 총 101개 균주 중에서 Aeromonas spp.가 44개(43.5%)로 가장 우점하였고, 그 다음으로 Pseudomonas spp. 4개(4.0%), Vibrio spp. 5개(5.0%)가 확인되었다. 또한, 다중 내성을 보이는 균주(77.2%)가 단일 내성균(22.8%)보다 많음을 확인하였다. tet(A), tet(D), tet(E), tet(G), tet(M), tet(S)가 TC 내성 균주에서 검출되었으며 tet(A)가 가장 우점적으로 확인되었다. Aeromonas spp.는 분리된 균주 중에서 가장 많았으며, 경기도 내 양식현장에서의 다양한 항생제 내성 유전자의 특성에 대한 추가적인 연구가 필요하다.

• 대한수의학회지
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• 제29권3호
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• pp.303-313
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• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.

• 한국동물위생학회지
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• 제45권3호
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• pp.181-189
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• 2022

Streptococcus is one of the major pathogen groups inducing bovine mastitis. The aim of this study was to investigate the antimicrobial resistance patterns of Streptococcus species isolated from bovine mastitis milk samples in Korea from 2016 to 2021. In total, 181 (10.3%) Streptococcal isolates were collected from 1,761 quarter milk samples at 122 farms; S. uberis 39.2% (n=71), S. dysgalactiae 29.3% (n=53), S. equinus 9.9% (n=18), S. suis 6.1% (n=11), S. parauberis 4.4% (n=8), S. lutetiensis 3.9% (n=7), others 7.2% (n=13). However, S. agalactiae was not isolated. The isolates showed the highest resistance rate to tetracycline (55.2%) followed by erythromycin (45.3%) and pirlimycin (36.5%). In contrast, all isolates were susceptible to ceftiofur, cephalothin, penicillin/novobiocin, and only single S. equinus isolate was resistant to both ampicillin and penicillin. Of 181 isolates, 64 (35.4%) were multidrug resistance (MDR). The resistance to pirlimycin of S. uberis (73.2%) was much higher than that of other species (0~36.4%). All S. suis isolates were resistance to tetracycline. S. dysgalactiae showed lower resistance to erythromycin, pirlimycin and tetracycline than S. uberis and S. suis. The rate of MDR was relatively higher among S. uberis (73.2%) than among S. suis (36.4%), S. dysgalactiae (15.1%), others (0%). In conclusion, antimicrobial resistance in Streptococcus spp. should be regularly examined for appropriate therapies because the resistance patterns were various among the individual species.

• 한국동물위생학회지
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• 제30권1호
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• pp.103-113
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• 2007

We monitored antibiotic resistance of Escherichia coli isolates from healthy volunteers of community and chicken butchers from February to July in 2006. From disc diffusion test on 473 E coli isolates from healthy volunteers of the community, the resistance rates of tetracycline, ampicillin, and ticarcillin were 44.8%, 40.8%, and 37.4%, respectively. In the disc diffusion test on 18 E coli isolates from chicken butchers, the resistance rates of tetracycline, nalidixic, streptomycin, and ampicillin were 94.4%, 61.1%, 55.6%, and 50.0%, respectively.

• 한국어병학회지
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• 제23권1호
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• pp.37-45
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• 2010

넙치 병원성 세균에 대한 항균제 내성율과 다제내성을 조사하고자 연구를 수행하였다. 우리나라의 양식 넙치 (Paralichthys olivaceus)에서 161균주가 분리되었고 분리균주에는 Edwardsiella tarda (n=32), Vibrio ichthyoenteri (n=37), Vibrio spp. (n=54), Streptococcus parauberis (n=28) 그리고 Streptococcus spp. (n=10)가 포함되었다. E. tarda 분리균주는 tetracycline (84.4%)과 oxolinic acid (71.9%)에 높은 내성율을 나타내었고 V. ichthyoenteri와 Vibrio spp.는 ampicillin (94.6%, 81.5%)과 tetracycline (56.8%, 42.6%) 순서로 높은 내성율을 나타내었다. S. parauberis 분리균주는 ampicillin (57.1%), tetracycline (57.1%), erythromycin (35.7%) 순서로 높은 내성율을 보였다. E. tarda의 84.4%, V. ichthyoenteri의 73.0%, Vibrio spp.의 57.4%, S. parauberis의 42.8% 그리고 Streptococcus spp.의 70.0%가 2종류 이상의 항균제에 다제내성을 나타내었다.

• 미생물학회지
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• 제46권4호
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• pp.326-333
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• 2010

2003년부터 2009년까지 서울지역 소아에서 분리된 nontyphoid Salmonella 105주에 대해서 혈청형, 항생제 내성양상, integron의 특징과 PFGE를 수행하였다. 혈청형은 총 18종으로 S.Enteritidis가 가장 많이 분리되었고, 그 다음은 Montevide였다. 항균제 내성은 혈청형별로 차이가 있었으나 전체 살모넬라에 대해서 10종의 항균제에 대한 내성률은 ampicillin이 60%로 가장 높았으며, tetracycline 46.7%, streptomycin 35.2%, nalidixic acid 28.6% 순이었다. 다재내성 유형을 알아본 결과 nalidixic acid 단독 내성이 15.7%로 가장 많았고, ampicillinampicillin/sulbactam-tetracycline형이 14.5%, ampicillin-streptomycin-chloramphenicol-tetracycline형이 10.8%였다. Integron에 대한 연구 결과 integron 보유율은 19%로 20주에서 class 1 integron을 가지고 있었고 gene cassette는 20%만 확인이 되었다. 확인된 gene cassette는 aadA2, blaP1과 dfrA12-aadA2, dfr17-aadA5, aadA7이였다. 연도별 분리균의 유연관계를 확인 하고자 가장 분리율이 높은 S. Enteritidis 50주에 대해서 PFGE를 수행한 결과 3가지 Pulsotype으로 나눠어졌다. 3주를 제외한 모든 균주는 similarity 89.8%의 비교적 유연관계가 높은 균임을 확인할 수 있었다.

• 약학회지
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• 제36권3호
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• pp.255-258
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• 1992

The clinical isolate Staphylococcus aureus SA8 was resistant to tetracycline(Tc) and harboured a plasmid pKH1(24.82 kb). pKH1 was shown by curing and by transformation to specify resistance to Tc. The cleavage map of a pKH1 was determined by restricction enzyme mapping techniques. Cleavage map is given for BglII, EcoRI, HpaII, PvuII and SalI. Restriction endonuclease BamHI, BglI, BstEII, HpaI, PstI, and XhoI have no sites on this plasmid. HaeIII, XbaI, and HindIII have 5, 6, 14 sites, respectively.

• 한국동물위생학회지
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• 제42권2호
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• pp.53-60
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• 2019

This study was performed to investigate antimicrobial resistance in bacterial isolates obtained from companion dogs in veterinary hospitals and an animal shelter in Incheon. Drug resistance was examined respectively with the isolates of Escherichia coli, Enterococcus faecalis, and Staphylococcus pseudintermedius. The prevalence of drug resistance was calculated for each bacterial species towards 163 E. coli isolates, 156 E. faecalis isolates, and 86 S. pseudintermedius isolates by using selected antimicrobials. E. coli isolates were highly resistant to ampicillin, ciprofloxacin and tetracycline (47.9%, 28.2% and 28.2%, respectively). E. faecalis isolates were highly resistant to quinupristin-dalfopristin, tetracycline, kanamycin, rifampicin (69.8%, 66.0%, 53.8% and 51.9%, respectively). Higher levels of resistance were detected for ampicillin, penicillin, tetracycline, erythromycin, trimethoprim/sulfamethoxazole, telithromycin in S. pseudintermedius isolates (83.7%~52.6%, respectively). Occurrence of methicillin-resistant S. pseudintermedius (MRSP) was confirmed by oxacillin disc diffusion method, resulted in 23.3% occurrence among the S. pseudintermedius isolates (20/86 strains). The occurrence ratio of multidrug-resistance in the isolates of E. coli, E. faecalis, and S. pseudintermedius was 34.5%, 56.9%, and 67.9%, respectively. In this study, higher levels of antimicrobial drug resistance were observed in bacterial isolates obtained from dogs in Incheon. A regular monitoring and surveillance program should be implemented to prevent the emergence and spread of the drug-resistant bacteria carried in companion dogs.

• 한국식품영양학회지
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• 제26권1호
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• pp.132-136
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• 2013

Antimicrobial resistance and multi-drug resistance patterns have been carried out on total of 210 isolated of Salmonella spp. and pathogenic E. coli isolated from food poisoning patients on January through December 2012 in Incheon, Korea. The highest percentage of antibiotics resistance was found to the following antimicrobial agents: tetracycline 43.8%, ampicillin 34.8%, nalidixic acid 23.8%, sulfamethoxazole/trimethoprim and chloramphenicol 12.4%, and ampicillin/sulbactam 11.4%. The highest percentage of resistance was 37.5% to ampicillin for Salmonella spp. and 59.0% to tetracycline for pathogenic E. coli. Overall the multidrug resistance rates of 1 drug was 26.2%, 2 drugs 9.0%, 3 drugs 9.5%, 4 drugs 7.1%, and 5 or more drugs 12.46%. The multi-drug (MDR) strains to four or more antimicrobial agents among the resistant organisms were quite high: 15.9% and 22.1% for Salmonella spp. and pathogenic E. coli, respectively. The study implies that limitation of unnecessary medication use is pertinent in order to maintaining the efficacy of drugs.