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http://dx.doi.org/10.7847/jfp.2021.34.1.039

Distribution of Tetracycline-Resistance Genes detected from isolates of cultured fishes in Gyeonggi-do  

Cho, Ki-Taek (Gyeonggi Province Maritime&Fisheries Research Institute)
Hwang, Yun-Jeong (Gyeonggi Province Maritime&Fisheries Research Institute)
Lee, Sang-Woo (Gyeonggi Province Maritime&Fisheries Research Institute)
Kim, Kwang-Il (Department of Aquatic Life Medicine, Pukyong National University)
Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.34, no.1, 2021 , pp. 39-46 More about this Journal
Abstract
Tetracycline (TC) is one of the antibiotics used for treatment of bacterial infection in Korea. Inadequate usage and abuse cause the resistance to antibiotics, like Tetracycline, Erythromycin, and Fluoroquinolone. It can also affect severe economic loss in aquaculture field in Korea. We isolated 101 bacterial samples from diseased fish at aquaculture sites in Gyeonggi-do during 2015~2018. Minimum inhibitory concentration (MIC) method has been used to determine distribution and to identify bacterial isolates resistant to antibiotics including Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN). TC resistant isolates were confirmed antibiotic resistant genes by conventional PCR. Bacterial isolates were identified as Aeromonas spp. (43.5%), Pseudomonas spp. (4.0%) and Vibrio spp. (5.0%). It was confirmed that multi-resistant isolates (77.2%) were predominant over single-resistant one (22.8%). TC resistant genes like tet(A), tet(D), tet(E), tet(G), tet(M), and tet(S) were detected and tet(A) was the most prevalent. Aeromonas spp. is a dominant strain in bacterial infections in fishes of aquaculture sites, and further investigation on various antibiotic resistance genes will be needed for clear understanding of aquaculture sites in Gyeonggi-do.
Keywords
Tetracycline; Antibiotic resistance gene; tet gene; Aeromonas spp.; Multi-resistant bacteria;
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