Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Characterization of Antimicrobial Resistance Patterns and Integrons of Nontyphoid Salmonella Isolates from Infants in Seoul

서울지역 소아에서 분리된 Nontyphoid Salmonella의 항생제 내성과 Integron의 특징

  • Jin, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Jin-Ah (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Ji-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jeon, Soo-Jin (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jae-Kyoo (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Oh, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Ki-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Young-Ki (Department of Public Health, Graduate School of Public Health & Social Welfare, Dankook University)
  • 진영희 (서울시보건환경연구원 역학조사팀) ;
  • 김진아 (서울시보건환경연구원 역학조사팀) ;
  • 정지헌 (서울시보건환경연구원 역학조사팀) ;
  • 전수진 (서울시보건환경연구원 역학조사팀) ;
  • 이재규 (서울시보건환경연구원 역학조사팀) ;
  • 오영희 (서울시보건환경연구원 역학조사팀) ;
  • 한기영 (서울시보건환경연구원 역학조사팀) ;
  • 이영기 (단국대학교 보건복지 대학원 공중보건학과)
  • Received : 2010.08.30
  • Accepted : 2010.10.12
  • Published : 2010.12.31
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Abstract

A total of 105 nontyphoid Salmonella isolated from infants in Seoul from 2003 to 2009 was investigated for their serotype, antimicrobial resistance, characterization of integron, and the patterns of Pulsed-field gel electrophoresis (PFGE). Eighteen serotypes were detected in 105 isolates, and the two most common serotypes were S. Enteritidis (47.6%) and Montevideo (15.2%). Among the Salmonella serovars, a high level of antimicrobial resistance was found to ampicilin (60%), tetracycline (46.7%), streptomycin (35.2%) and nalidixic acid (28.6%). In the multi-drug resistance patterns, the predominant patterns were only nalidixic acid (15.7%), ampicillin-ampicillin/sulbactam-tetracycline (14.5%), and ampicillin-streptomycin-chloramphenicol-tetracycline (10.8%). PCR and DNA sequencing analysis revealed the presence of class 1 integron in 20 isolates (19%). Of the class 1 integron positive isolates 20% harboured the integron-associated gene cassettes : aadA2, blaP1, dfr17-aadA5, dfrA12-aadA2, and aadA7. PFGE was carried out to examine the genetic relatedness among S. Enteritidis isolates. Except for three strains, fifty strains were divided by three pulsotypes.

2003년부터 2009년까지 서울지역 소아에서 분리된 nontyphoid Salmonella 105주에 대해서 혈청형, 항생제 내성양상, integron의 특징과 PFGE를 수행하였다. 혈청형은 총 18종으로 S.Enteritidis가 가장 많이 분리되었고, 그 다음은 Montevide였다. 항균제 내성은 혈청형별로 차이가 있었으나 전체 살모넬라에 대해서 10종의 항균제에 대한 내성률은 ampicillin이 60%로 가장 높았으며, tetracycline 46.7%, streptomycin 35.2%, nalidixic acid 28.6% 순이었다. 다재내성 유형을 알아본 결과 nalidixic acid 단독 내성이 15.7%로 가장 많았고, ampicillinampicillin/sulbactam-tetracycline형이 14.5%, ampicillin-streptomycin-chloramphenicol-tetracycline형이 10.8%였다. Integron에 대한 연구 결과 integron 보유율은 19%로 20주에서 class 1 integron을 가지고 있었고 gene cassette는 20%만 확인이 되었다. 확인된 gene cassette는 aadA2, blaP1과 dfrA12-aadA2, dfr17-aadA5, aadA7이였다. 연도별 분리균의 유연관계를 확인 하고자 가장 분리율이 높은 S. Enteritidis 50주에 대해서 PFGE를 수행한 결과 3가지 Pulsotype으로 나눠어졌다. 3주를 제외한 모든 균주는 similarity 89.8%의 비교적 유연관계가 높은 균임을 확인할 수 있었다.

Keywords

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