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Characterization of Antimicrobial Resistance Patterns and Integrons of Nontyphoid Salmonella Isolates from Infants in Seoul  

Jin, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Jin-Ah (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Jung, Ji-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Jeon, Soo-Jin (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Jae-Kyoo (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Oh, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Han, Ki-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Young-Ki (Department of Public Health, Graduate School of Public Health & Social Welfare, Dankook University)
Publication Information
Korean Journal of Microbiology / v.46, no.4, 2010 , pp. 326-333 More about this Journal
Abstract
A total of 105 nontyphoid Salmonella isolated from infants in Seoul from 2003 to 2009 was investigated for their serotype, antimicrobial resistance, characterization of integron, and the patterns of Pulsed-field gel electrophoresis (PFGE). Eighteen serotypes were detected in 105 isolates, and the two most common serotypes were S. Enteritidis (47.6%) and Montevideo (15.2%). Among the Salmonella serovars, a high level of antimicrobial resistance was found to ampicilin (60%), tetracycline (46.7%), streptomycin (35.2%) and nalidixic acid (28.6%). In the multi-drug resistance patterns, the predominant patterns were only nalidixic acid (15.7%), ampicillin-ampicillin/sulbactam-tetracycline (14.5%), and ampicillin-streptomycin-chloramphenicol-tetracycline (10.8%). PCR and DNA sequencing analysis revealed the presence of class 1 integron in 20 isolates (19%). Of the class 1 integron positive isolates 20% harboured the integron-associated gene cassettes : aadA2, blaP1, dfr17-aadA5, dfrA12-aadA2, and aadA7. PFGE was carried out to examine the genetic relatedness among S. Enteritidis isolates. Except for three strains, fifty strains were divided by three pulsotypes.
Keywords
antimicrobial resistance; gene cassette; integron; nontyphoid Salmonella; PFGE;
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