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Current status and prospects of molecular marker development for systematic breeding program in citrus (감귤 분자육종을 위한 분자표지 개발 현황 및 전망)

  • Kim, Ho Bang;Kim, Jae Joon;Oh, Chang Jae;Yun, Su-Hyun;Song, Kwan Jeong
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.261-271
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    • 2016
  • Citrus is an economically important fruit crop widely growing worldwide. However, citrus production largely depends on natural hybrid selection and bud sport mutation. Unique botanical features including long juvenility, polyembryony, and QTL that controls major agronomic traits can hinder the development of superior variety by conventional breeding. Diverse factors including drastic changes of citrus production environment due to global warming and changes in market trends require systematic molecular breeding program for early selection of elite candidates with target traits, sustainable production of high quality fruits, cultivar diversification, and cost-effective breeding. Since the construction of the first genetic linkage map using isozymes, citrus scientists have constructed linkage maps using various DNA-based markers and developed molecular markers related to biotic and abiotic stresses, polyembryony, fruit coloration, seedlessness, male sterility, acidless, morphology, fruit quality, seed number, yield, early fruit setting traits, and QTL mapping on genetic maps. Genes closely related to CTV resistance and flesh color have been cloned. SSR markers for identifying zygotic and nucellar individuals will contribute to cost-effective breeding. The two high quality citrus reference genomes recently released are being efficiently used for genomics-based molecular breeding such as construction of reference linkage/physical maps and comparative genome mapping. In the near future, the development of DNA molecular markers tightly linked to various agronomic traits and the cloning of useful and/or variant genes will be accelerated through comparative genome analysis using citrus core collection and genome-wide approaches such as genotyping-by-sequencing and genome wide association study.

Uptake of Butachlor by Rice Seedlings and Its Phytotoxic Action to the Physiological Activities (수도묘(水稻苗)의 Butachlor 흡수(吸收) 및 약해발생(藥害發生) 특성(特性)에 관한 생리적(生理的) 연구(硏究))

  • Chung, Bong-Jin;Kwon, Yong-Woong
    • Korean Journal of Weed Science
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    • v.1 no.1
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    • pp.57-68
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    • 1981
  • To clarify the mode of uptake of butachlor (2-chloro-2', 6'-diethyl-N-(butoxymethyl) acetanilide) by rice seedlings, its phytotoxic action to growth and physiological activities, studies were conducted with rice seedlings, at the 6th or 7th leaf-stage, which were treated with nutrient solution containing butachlor 0, 1.8, 3.6, 7.2, 10.8 or 14.4 ppm for 1, 2 or 4 days, in other case, the solutions were thereafter renewed with the untreated nutrient solution for further growth. Uptake of butachlor by rice seedlings increased linearly with increase of its concentration and duration of uptake. Butachlor inhibited root growth more than shoot growth, furthermore, the inhibitory effect on the shoot growth was greater in height than in weight or leafing rate. After 4 day-treatment, the rates of shoot growth in weight were delayed for 4 days. Butachlor inhibited water uptake rapidly and linearly with increase of its external concentration. The reduced uptake of water was followed by slow increase in the stomatal resistance of leaves. Upon completion of butachlor treatment, rate of water uptake was recovered rapidly, but the stomatal resistance with lag in time. Butachlor did not affect the uptake of cation such as ammonium, potassium and calcium, but inhibited substantially uptake of nitrate in proportion to its concentration. Especially, butachlor did not affect synthesis and degradation of nitrate reductase. In addition, butachlor has shown much greater binding to the lipidic substances from rice roots than the proteinous material. The primary mechanism of phytotoxic action of butachlor does not seem to be its effect on the protein synthesis, but great affinity to membranes. The inhibition of water uptake, and its subsequent closure of stomates is thought very important for reduced growth under mild phytotoxicity.

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Genetic Variance and Combining Ability of Shattering and Growth Characters by Diallel Crosses of Sesame (이면교잡에 의한 참깨의 탈립성 및 생육형질의 유전변이와 조합능력)

  • Kim, Dong-Hwi;Kang, Chul-Whan;Shim, Kang-Bo;Park, Chang-Hwan;Lee, Sung-Woo;Seong, Nak-Sul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.7
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    • pp.652-657
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    • 2006
  • Combining ability and heterosis for $F_{1}$ population obtained $5{\times}5$ half diallel cross in sesame were estimated for six quantitative characters. The parental varieties used for diallel cross were Yangbaek and Ansan of normal type, Suwon 177 and 195 of placenta adhesion type (PA) and SIG960320-5-1-1 of indehiscent type (ID). PA and ID type are shattering-resistant sesames and Yangbaek and Ansan are shattering sesames. The high heterosis of $F_{1}$ hybrids was expressed in the number of capsules per plant (NC), grain yield per plant (GY) and number of branches per plant (NB). Shattering.ate (SR) showed high positive heterosis toward increase of seeds shattering in all the combination of $F_{1}$ generation which was presumed because the characters connected with shattering resistance of seeds were governed by recessive gene. At the analysis of combining ability, highly significant general combining ability (GCA) effects were observed on all the characters in $F_{1}$ generation, significant specific combining ability (SCA) effects were observed on the NC, GY and SR. GCA variances were greater than SCA variance, which suggested that additive gene effects were greater than other nonadditive gene effects. Considering the estimated GCA effects, Suwon 195 (PA type) was found to be good parent for SR and GY, SIG960320-5-1-1 (ID type) was poor for GY and plant height. SCA effect for NC was great in Yangbaek/Suwon 195, SCA for GY was great in Ansan/SIG960320-5-1-1, and SCA for decrease of SR was great in Ansan/Suwon177.

Breakthrough Urinary Tract Infection: A Clinical Study of Experience of a Single Center (예방적 항생제 사용중에 발생한 요로감염: 단일 병원에서 경험한 임상연구)

  • Bae, Sang-In;Cheon, Chong-Kun;Kim, Su-Young
    • Childhood Kidney Diseases
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    • v.14 no.2
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    • pp.203-209
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    • 2010
  • Purpose : It has been a common medical practice to use prophylactic antibiotics to prevent recurrent urinary tract infections (UTI) in high risk situations such as urinary tract obstruction, vesicoureteral reflux, neurogenic bladder, or urinary stones. But sometimes, we meet difficult situation of breakthrough infections (BI) which might cause new or progressive renal scarring. The clinical characteristics of children contracting breakthrough UTI experienced in a single center were studied. Methods : The study was done retrospectively through medical records of 150 pediatric patients who had been cared in pediatric and urologic clinics of Pusan National University Hospital from Jan. 2001 till June 2006 and had prophylactic antibiotics to prevent recurrent UTI. Results : The starting age of prophylactic antibiotics of 150 patient was 1-76 months, and median age was 5 months. The BI developed 61 times in 43 patients (28.7%), 1.5 times per 100 patient-months. The BI occurred more frequently in patients with higher grade of VUR, and in the cases with abnormal DMSA scan. Co-trimoxazole was more effective than 2nd and 3rd generation cephalosporins to prevent UTI. The distribution of causative organisms was more diverse than usual UTI. The causative organisms were sensitive to the antibiotics used for prophylaxis in 29.5%, and resistant in 59.1%. After experience of BI, 40 percents of patients went to the surgical treatment including endoscopic injection of Deflux, 35% to new antibiotics for prophylaxis, 26% remain on the same antibiotics as the previous one. Conclusion : Based on our study results, preexisting renal scar might be one of the factors which should be considered in favor of early surgical interventions of VUR. Poor compliance and wrong selection of antibiotics such as cephalosporins are important underlying causes of breakthrough UTIs.

Peritonitis in Children Undergoing Peritoneal Dialysis: 10 Years' Experience in a Single Center (소아 복막 투석 환자에서 발생한 복막염: 단일기관에서 10년간의 경험)

  • Lee, Se-Eun;Han, Kyoung-Hee;Jung, Yun-Hye;Lee, Hyun-Kyung;Kang, Hee-Gyung;Cheong, Hae-Il;Ha, Il-Soo
    • Childhood Kidney Diseases
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    • v.14 no.2
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    • pp.174-183
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    • 2010
  • Purpose : The organisms causing peritonitis and their antibiotic sensitivities vary in different regions and centers, and these data are necessary to establish regional treatment guidelines. The aim of this study was to investigate the changes in incidence and characteristics of the organisms that cause peritonitis in children undergoing peritoneal dialysis (PD) during recent 10 years. Methods : We retrospectively collected and analyzed the data from medical records of 110 children on PD during the period from 2000 to 2010. Results : One hundred and forty episodes of peritonitis have occurred in 57 patients. The overall incidence of peritonitis was 0.43 episodes/patient year, and similar incidence have been maintained since 2003. Sixty percent of the patients experienced peritonitis within 1 year of PD, and all patients commencing PD in infancy experienced peritonitis. Gram positive (G (+)), gram negative (G (-)) organisms and fungi were cultured in 58%, 38%, and 4.1% respectively and cultures were negative in 13.6%. Staphylococcus was the most common G (+) organism, and Pseudomonas and Acinetobacter were 2 most frequent G (-) organisms isolated. Fifty-six percent of the G (+) organisms were sensitive to first generation cephalosporin and 91% of G (-) pathogens were sensitive to ceftazidime. Methicillin-resistance rate was not higher in children less than 2 years of age than in those more than 2 years. Conclusion : An additional breakthrough has to be made to further reduce the incidence of peritonitis. Treatment guideline customized for peritonitis in Korean children on PD need to be established through a nationwide co-work.

A Study on the Neumann-Kelvin Problem of the Wave Resistance (조파저항에서의 Neumann-Kelvin 문제에 대한 연구)

  • 김인철
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.21 no.2
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    • pp.131-136
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    • 1985
  • The calculation of the resulting fluid motion is an important problem of ship hydrodynamics. For a partially immersed body the condition of constant pressure at the free surface can be linearized. The resulting linear boundary-value problem for the velocity potential is the Neumann-Kelvin problem. The two-dimensional Neumann-Kelvin problem is studied for the half-immersed circular cylinder by Ursell. Maruo introduced a slender body approach to simplify the Neumann-Kelvin problem in such a way that the integral equation which determines the singularity distribution over the hull surface can be solved by a marching procedure of step by step integration starting at bow. In the present pater for the two-dimensional Neumann-Kelvin problem, it has been suggested that any solution of the problem must have singularities in the corners between the body surface and free surface. There can be infinitely many solutions depending on the singularities in the coroners.

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Effect of soil temperature on the growth and dry matter accumulationof sorghum and corn (토양온도가 수수와 옥수수의 생육 및 건물축적에 미치는 영향)

  • 한흥전;한민수;김정갑
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.6 no.3
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    • pp.179-184
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    • 1986
  • The pot trial was carried out to investigate the influence of soil temperature on the growth and dry matter accumulation in sorghum cv. Pioneer 931 and corn cv. Suweon 19 during their growing season in 1985. Soil temperature maintained with low ($22.3^{\circ}C$), natural($25.9^{\circ}C$) and high temperature($30.5^{\circ}C$) over 24 hour. The results are summarized as follows; 1. Growth of sorghum plants was associated with increasing of soil temperature, while that of corn was reduced under high temperature($30.5^{\circ}C$). The highest plant height of corn was found at natural temperature($25.9^{\circ}C$). Sorghum had higher growth rate than corn plants in all temperature levels. 2. Assimirable leaf areas of the plants were greately increased under high temperature both in sorghum and corn. Sorghum plants maintained almost same levels of leaf area during maturity stage, but those of corn were markedly decreased after mild stage. 3. The highest rates of photosynthesis were found at nature' soil temperature ($25.9^{\circ}C$) both in sorghum($1.619mg/s/m^2$) and corn plants($1.084mg/s/m^2$). Under high temperature($30.5^{\circ}C$) the photosynthesis rates of the plants were decreased to about 1.553 mg and 0.404 mg for sorghum and corn, respectively. Low temperature ($22.3^{\circ}C$) produced only a value of 0.775 mg for sorghum and 0.048 mg for corn. 4. Seasonal dry matter accumulation of sorghum increased in the order of high>natural>low soil temperature, but that of corn increased in the order of natural>high>low soil temperature, respectively.

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Comparative Study on the Productivity for Silage Corn (Zea mays L.) Variety Certified Import Adaptability in Pyeongchang Area (사일리지용 옥수수 수입적응성 인증 품종의 평창지역 생산성 비교 연구)

  • Kim, Jong Geun;Li, Yu Wei;Park, Hyung Soo;Kim, Jong Duk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.2
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    • pp.161-167
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    • 2017
  • This experiment was conducted to comparison study on the productivity for certified varieties of import adaptability of silage corn in Pyeongchang area. Total eight varieties (Gangpyeongok, 31N27, 32P75, 32W86, P3156, P3394, DK 689 및 DK 729) were evaluated. The experimental design was 8 treatment of randomized block with three replications. Corn varieties were cultivated in experimental field of Pyeongchang campus, SNU from 1 May to 2 September, 2015 and plot size was $15m^2$. Plant height of 32W86 and ear height of Gwanpyeongok was the highest (p<0.05). Tasseling and silking date was 27 July-3 August and silking occurred after 1-3 day of tasseling. Average day to silking was 92 days and that of 31N27 variety was short (p<0.05). The varieties of DeKalbo Company (DK 689 and DK 729) required more times to silking. Average GDD (growth degree day) of eight varieties was $1,023^{\circ}C$ and P3352 was the lowest GDD. In the trials of resistance evaluation, P3394 was strong in disease, P3156 was the highest in insect. All varieties did not show the lodging and variety of DK 729 showed the highest stay green score (p<0.05). Average dry matter content was 30.77%, it showed higher trend in DM. 32W86 was the highest DM content among the varieties, but there was no significant difference among varieties (p>0.05). The weight per ear was the highest in 32W86 and the lowest in Gwanpyeongok. The ration of ear to whole plant was higher in 32W86 and P3394, but it was not found the significant difference (p>0.05). Average yield of fresh and DM was 59,017 and 13,476 kg/ha, respectively. DK 689 showed higher DM and TDN yield than others, but there was not significant difference (p>0.05). According to results, the difference of productivity was not found among certified variety of import adaptability of silage corn. The varieties Gwanpyeongok, 32W86 and 32P75 would be recommendable in Pyeongchang area for stable cultivation.

The Effects of the Intensity of Combined Training on Body Composition, HOMA-IR and HbA1c of Female Students of a Boarding High School (복합운동 강도가 기숙형학교 여고생의 신체조성, HOMA-IR 및 HbA1c에 미치는 영향)

  • Kwon, Sun-Ok;Jeong, Seon-Tae
    • Journal of Life Science
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    • v.20 no.1
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    • pp.124-132
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    • 2010
  • Among students of 'K' boarding high school, located in 'B' city, 32 students whose % body fat was 30% or above were divided into three groups - two exercise groups and one control group. They performed Combined Training - a mix of weight training (WT) and step box training (SBT) - for 65 min a day, 3 days a week, for 8 weeks in total. Group A performed WT 70-80%$RM{\times}3$ sets+SBT (RPE 11-13)${\times}1$ set, and group B performed WT 70-80%$RM{\times}1$ set+SBT (RPE 11-13)${\times}3$ sets to yield data on changes of body composition (Soft Lean Mass, SLM), %fat, WHR), HbA1c, and HOMA-IR. Paired t-test was used to process data within each group. Pre- and post experiment differences rates (%diff) were used to perform one-way ANOVA (Duncan test) for group comparisons. The conclusions derived are as follows. Regarding body composition, exercise groups showed an increase in SLM, but there was no such change in the control group. WHR decreased in group A, but increased in the control group. The % body fat decreased in both exercise groups, but increased in the control group. As for the group comparisons, SLM in group A showed a greater increase than in group B and the control group. WHR in groups A and B showed a greater decrease than the control group. The % body fat in groups A and B showed a greater decrease than the control group. The exercise groups showed a significant decrease in HOMA-IR, but the control group showed a significant increase in HOMA-IR. As for the group comparisons, groups A and B showed a greater decrease in HOMA-IR than the control group. The exercise groups showed a significant decrease in HbA1c, however, the control group showed no change in HbA1c. As for the group comparisons, group A showed a greater decrease in HbA1c than the control group. These results confirm that combined training is more effective in improving body composition and metabolic factors when it includes a high proportion of resistance training, rather than aerobic exercise. The results of the study suggest that it is advisable to set a high proportion of WT when deciding the intensity of combined training.

Thermal Property and Fire Resistance of Cellulose Insulation (섬유질 단열재의 열적 특성 및 내화성능)

  • Kwon, Young-Cheol;Seo, Seong Yeon;Kim, Sung Young
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.9 no.3
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    • pp.203-212
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    • 2005
  • Cellulose insulation is primarily manufactured from recycled newsprint and treated with fire retardants for the fire resistance. Thanks to the fire retardants, it is not combustible and flammable. In addition to that, Its thermal resistance is much better than that of fiberglass or rock wool. It is made from waste paper and easily decayed when it is demolished, and it has small embodied energy. So it is very environment-friendly building material. For broader use of cellulose insulation in buildings in Korea, it is necessary to test its physical performance to compare the results with the requirements on the Korean Building Code. To this end, apparent thermal conductivity (ka) measurements of Korean-made loose-fill cellulose insulations were recently completed using equipment that was built and operated in accordance with ASTM C 518 and the fire resistance was tested in accordance with ASTM C 1485. Korean loose-fill cellulose has thermal conductivity about 5% greater than the corresponding U.S. product at the same density. This is likely due to differences in the recycled material being used. Both spray-applied and loose-fill cellulose insulation lose about 1.5% of their thermal resistivity for $5.5^{\circ}C$ increase in temperature. The fire resistance of cellulose insulation is increased in linear proportion to the increase of the rate of fire retardant. Thanks to the high fire resistance, cellulose insulation can be used as a substitution of Styrofoam or Urethane foam which is combustible. The thermal conductivity of cellulose insulation was $0.037-0.043W/m{\cdot}K$ at the mean specimen temperature from $4-43^{\circ}C$. It corresponds to the thermal resistance of "Na Grade" according to the Korean Building Code. The effect of chemical content on thermal conductivity was negligible for all but the chemical-free specimen which had the highest value for the thermal conductivity over the temperature range tested. The thermal resistance of cellulose insulation is better than that of fiberglass or rock wool, and its fire resistance is higher than that of Styrofoam or Urethane foam. Therefore it can be substituted for those above considering its physical performance. Cellulose insulation is no more expensive than Styrofoam or rock wool, so it is recommended to use it more widely in Korea.