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http://dx.doi.org/10.5010/JPB.2016.43.3.261

Current status and prospects of molecular marker development for systematic breeding program in citrus  

Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co. Ltd.)
Kim, Jae Joon (Life Sciences Research Institute, Biomedic Co. Ltd.)
Oh, Chang Jae (Life Sciences Research Institute, Biomedic Co. Ltd.)
Yun, Su-Hyun (Citrus Research Institute, National Institute of Horticultural & Herbal Science)
Song, Kwan Jeong (Major of Horticultural Science, Faculty of Bioscience and Industry, Jeju National University)
Publication Information
Journal of Plant Biotechnology / v.43, no.3, 2016 , pp. 261-271 More about this Journal
Abstract
Citrus is an economically important fruit crop widely growing worldwide. However, citrus production largely depends on natural hybrid selection and bud sport mutation. Unique botanical features including long juvenility, polyembryony, and QTL that controls major agronomic traits can hinder the development of superior variety by conventional breeding. Diverse factors including drastic changes of citrus production environment due to global warming and changes in market trends require systematic molecular breeding program for early selection of elite candidates with target traits, sustainable production of high quality fruits, cultivar diversification, and cost-effective breeding. Since the construction of the first genetic linkage map using isozymes, citrus scientists have constructed linkage maps using various DNA-based markers and developed molecular markers related to biotic and abiotic stresses, polyembryony, fruit coloration, seedlessness, male sterility, acidless, morphology, fruit quality, seed number, yield, early fruit setting traits, and QTL mapping on genetic maps. Genes closely related to CTV resistance and flesh color have been cloned. SSR markers for identifying zygotic and nucellar individuals will contribute to cost-effective breeding. The two high quality citrus reference genomes recently released are being efficiently used for genomics-based molecular breeding such as construction of reference linkage/physical maps and comparative genome mapping. In the near future, the development of DNA molecular markers tightly linked to various agronomic traits and the cloning of useful and/or variant genes will be accelerated through comparative genome analysis using citrus core collection and genome-wide approaches such as genotyping-by-sequencing and genome wide association study.
Keywords
Citrus; Genomics; Genetic Map; Molecular Breeding; Molecular Markers; Traits;
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