• Transactions on Electrical and Electronic Materials
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• v.5 no.4
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• pp.138-142
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• 2004

This paper presents the improved bum-in method for the reliability of SRAM in Multi Chip Package (MCP). Semiconductor reliability is commonly improved through the bum-in process. Reliability problem is more significant in MCP that includes over two chips in a package, because the failure of one chip (SRAM) has a large influence on the yield and quality of the other chips - Flash Memory, DRAM, etc. Therefore, the quality of SRAM must be guaranteed. To improve the quality of SRAM, we applied the improved wafer level bum-in process using multi cells selection method in addition to the previously used methods. That method is effective in detecting special failure. Finally, with the composition of some kind of methods, we could achieve the high quality of SRAM in Multi Chip Package.

• Molecules and Cells
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• v.45 no.11
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• pp.820-832
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• 2022

As a potential candidate to generate an everlasting cell source to treat various diseases, embryonic stem cells are regarded as a promising therapeutic tool in the regenerative medicine field. Cohesin, a multi-functional complex that controls various cellular activities, plays roles not only in organizing chromosome dynamics but also in controlling transcriptional activities related to self-renewal and differentiation of stem cells. Here, we report a novel role of the α-kleisin subunits of cohesin (RAD21 and REC8) in the maintenance of the balance between these two stem-cell processes. By knocking down REC8, RAD21, or the non-kleisin cohesin subunit SMC3 in mouse embryonic stem cells, we show that reduction in cohesin level impairs their self-renewal. Interestingly, the transcriptomic analysis revealed that knocking down each cohesin subunit enables the differentiation of embryonic stem cells into specific lineages. Specifically, embryonic stem cells in which cohesin subunit RAD21 were knocked down differentiated into cells expressing neural alongside germline lineage markers. Thus, we conclude that cohesin appears to control the fate determination of embryonic stem cells.

• Journal of Microbiology and Biotechnology
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• v.34 no.4
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• pp.854-862
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• 2024

Lactobacillus is a commonly used probiotic, and many researchers have focused on its stress response to improve its functionality and survival. However, studies on persister cells, dormant cells that aid bacteria in surviving general stress, have focused on pathogenic bacteria that cause infection, not Lactobacillus. Thus, understanding Lactobacillus persister cells will provide essential clues for understanding how Lactobacillus survives and maintains its function under various environmental conditions. We treated Lactobacillus strains with various antibiotics to determine the conditions required for persister formation using kill curves and transmission electron microscopy. In addition, we observed the resuscitation patterns of persister cells using single-cell analysis. Our results show that Lactobacillus creates a small population of persister cells (0.0001-1% of the bacterial population) in response to beta-lactam antibiotics such as ampicillin and amoxicillin. Moreover, only around 0.5-1% of persister cells are heterogeneously resuscitated by adding fresh media; the characteristics are typical of persister cells. This study provides a method for forming and verifying the persistence of Lactobacillus and demonstrates that antibiotic-induced Lactobacillus persister cells show characteristics of dormancy, sensitivity of antibiotics, same as exponential cells, multi-drug tolerance, and resuscitation, which are characteristics of general persister cells. This study suggests that the mechanisms of formation and resuscitation may vary depending on the characteristics, such as the membrane structure of the bacterial species.

• Journal of Communications and Networks
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• v.10 no.2
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• pp.127-136
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• 2008

For mobile wireless systems with full frequency reuse, co-channel interference near the cell coverage boundaries has a significant impact on the signal reception performance. This paper addresses an approach to efficiently mitigate the effect of downlink co-channel interference when multi-antenna terminals are used in cellular environments, by proposing a signal detection strategy combined with a system-level coordination for dynamic frequency reuse. We demonstrate the utilization of multi-antennas to perform spatial demultiplexing of both the desired signal and interfering signals from adjacent cells results in significant improvement of spectral efficiency compared to the maximal ratio combining (MRC) performance, especially when an appropriate frequency reuse based on the traffic loading condition is coordinated among cells. Both analytic expressions for the capacity and experimental results using the adaptive modulation and coding (AMC) are used to confirm the performance gain. The robustness of the proposed scheme against varying operational conditions such as the channel estimation error and shadowing effects are also verified by simulation results.

• Journal of the Korean institute of surface engineering
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• v.48 no.4
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• pp.169-173
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• 2015

$CuInSe_2$ thin films which have been widely used for thin solar cells as a light absorber were prepared by hydrazine solution processing, and their microstructural properties were investigated. Hydrazine $CuInSe_2$ precursor solutions were prepared by dissolving $Cu_2S$, S, $In_2Se_3$ and Se powder in hydrazine solvent. Multilayer $CuInSe_2$ chalcopyrite phase thin films were prepared by repeating spin-coating process using the precursor solution. Unfortunately, the presence of the interfaces between each $CuInSe_2$ layer formed by multi-layer coating impeded grain growth across the interface. Here, by doing simple interface engineering to solve the limited grain growth issue, the large grained (${\sim}1{\mu}m$) $CuInSe_2$ thin films were obtained.

• Journal of international Conference on Electrical Machines and Systems
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• v.1 no.1
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• pp.99-103
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• 2012

This paper presents a new switch-control approach for multiphase interleaved converters. Development of the proposed technique is based on control-signal generation for multi-unit synchronization. Current sharing among the switching cells and voltage regulation are achieved by means of a multi-loop control scheme regardless of changes in input voltage and load. System stability is ensured by single-pole compensation. This proposed technique is straightforward, reliable and inexpensive, and can be applied to any higher number of cells without difficulty.

• Molecules and Cells
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• v.46 no.2
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• pp.120-129
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• 2023

Recent technical advances have enabled unbiased transcriptomic and epigenetic analysis of each cell, known as "single-cell analysis". Single-cell analysis has a variety of technical approaches to investigate the state of each cell, including mRNA levels (transcriptome), the immune repertoire (immune repertoire analysis), cell surface proteins (surface proteome analysis), chromatin accessibility (epigenome), and accordance with genome variants (eQTLs; expression quantitative trait loci). As an effective tool for investigating robust immune responses in coronavirus disease 2019 (COVID-19), many researchers performed single-cell analysis to capture the diverse, unbiased immune cell activation and differentiation. Despite challenges elucidating the complicated immune microenvironments of chronic inflammatory diseases using existing experimental methods, it is now possible to capture the simultaneous immune features of different cell types across inflamed tissues using various single-cell tools. In this review, we introduce patient-based and experimental mouse model research utilizing single-cell analyses in the field of chronic inflammatory diseases, as well as multi-organ atlas targeting immune cells.

• Water Engineering Research
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• v.1 no.2
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• pp.147-158
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• 2000

In this study, we characterized the seasonal variation of rainrate fields in the Han river basin using the WGR multi-dimensional precipitation model (Waymire, Gupta, and Rodriguez-Iturbe, 1984) by estimating and comparing the parameters derived for each month and for the plain area, the mountain area and overall basin, respectively. The first-and second-order statistics derived from observed point gauge data were used to estimate the model parameters based on the Davidon-Fletcher-Powell algorithm of optimization. As a result of the study, we can find that the higher rainfall amount during summer is mainly due to the arrival rate of rain bands, mean number of cells per cluster potential center, and raincell intensity. However, other parameters controlling the mean number of rain cells per cluster, the cellular birth rate, and the mean cell age are found invariant to the rainfall amounts. In the application to the downstream plain area and upstream mountain area of the Han river basin, we found that the number of storms in the mountain area was estimated a little higher than that in the plain area, but the cell intensity in the mountain area a little lower than that in the plain area. Thus, in the mountain area more frequent but less intense storms can be expected due to the orographic effect, but the total amount of rainfall in a given period seems to remain the same.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.15 no.3
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• pp.66-71
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• 2016

Reducing the wafer breakage rate and sawing thinner wafers will decrease the cost of solar cells. This study was carried out in order to identify ways to achieve this goal. In this study, the cutting force characteristics using an ingot tilting-type diamond multi wire-sawing machine were analyzed. The cutting force was analyzed while varying the tilting angles and wire speed. The obtained data were analyzed by classifying the tangential cutting force and the normal cutting force. In this cutting force experiment, the difference between the forces was confirmed; it was found that it rises with increasing the tilting angles and decreases when the wire speed elevates. The resulting value can be utilized as basic data for the determination of an ideal cutting recipe.

• International Journal of Aeronautical and Space Sciences
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• v.18 no.4
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• pp.601-613
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• 2017

An adjoint-based error estimation and mesh adaptation study is conducted for two-dimensional viscous flows on unstructured hybrid meshes. The error in an integral output functional of interest is estimated by a dot product of the residual vector and adjoint variable vector. Regions for the mesh to be adapted are selected based on the amount of local error at each nodal point. Triangular cells in the adaptive regions are refined by regular refinement, and quadrangular cells near viscous walls are bisected accordingly. The present procedure is applied to single-element airfoils such as the RAE2822 at a transonic regime and a diamond-shaped airfoil at a supersonic regime. Then the 30P30N multi-element airfoil at a low subsonic regime with a high incidence angle (${\alpha}=21deg.$) is analyzed. The same level of prediction accuracy for lift and drag is achieved with much less mesh points than the uniform mesh refinement approach. The detailed procedure of the adjoint-based mesh refinement for the multi-element airfoil case show that the basic flow features around the airfoil should be resolved so that the adjoint method can accurately estimate an output error.