Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Single-Cell Genomics for Investigating Pathogenesis of Inflammatory Diseases

  • Seyoung Jung (Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Jeong Seok Lee (Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST))
  • Received : 2023.01.01
  • Accepted : 2023.01.24
  • Published : 2023.02.28
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Abstract

Recent technical advances have enabled unbiased transcriptomic and epigenetic analysis of each cell, known as "single-cell analysis". Single-cell analysis has a variety of technical approaches to investigate the state of each cell, including mRNA levels (transcriptome), the immune repertoire (immune repertoire analysis), cell surface proteins (surface proteome analysis), chromatin accessibility (epigenome), and accordance with genome variants (eQTLs; expression quantitative trait loci). As an effective tool for investigating robust immune responses in coronavirus disease 2019 (COVID-19), many researchers performed single-cell analysis to capture the diverse, unbiased immune cell activation and differentiation. Despite challenges elucidating the complicated immune microenvironments of chronic inflammatory diseases using existing experimental methods, it is now possible to capture the simultaneous immune features of different cell types across inflamed tissues using various single-cell tools. In this review, we introduce patient-based and experimental mouse model research utilizing single-cell analyses in the field of chronic inflammatory diseases, as well as multi-organ atlas targeting immune cells.

Keywords

Acknowledgement

This work was supported by a grant from the National Research Foundation of Korea (2022R1A4A3034038, 2022M3A9D3016848, and NRF-2022R1C1C1012634).

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