Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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The Kleisin Subunits of Cohesin Are Involved in the Fate Determination of Embryonic Stem Cells

  • Received : 2021.12.09
  • Accepted : 2022.07.24
  • Published : 2022.11.30
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Abstract

As a potential candidate to generate an everlasting cell source to treat various diseases, embryonic stem cells are regarded as a promising therapeutic tool in the regenerative medicine field. Cohesin, a multi-functional complex that controls various cellular activities, plays roles not only in organizing chromosome dynamics but also in controlling transcriptional activities related to self-renewal and differentiation of stem cells. Here, we report a novel role of the α-kleisin subunits of cohesin (RAD21 and REC8) in the maintenance of the balance between these two stem-cell processes. By knocking down REC8, RAD21, or the non-kleisin cohesin subunit SMC3 in mouse embryonic stem cells, we show that reduction in cohesin level impairs their self-renewal. Interestingly, the transcriptomic analysis revealed that knocking down each cohesin subunit enables the differentiation of embryonic stem cells into specific lineages. Specifically, embryonic stem cells in which cohesin subunit RAD21 were knocked down differentiated into cells expressing neural alongside germline lineage markers. Thus, we conclude that cohesin appears to control the fate determination of embryonic stem cells.

Keywords

Acknowledgement

This work was supported by grants from the National Research Foundation of Korea, funded by the Ministry of Science, ICT & Future Planning (No. 2020R1A2C2011887; 2018R1D1A1B07050755), the Korea Environment Industry & Technology Institute through "Digital Infrastructure Building Project for Monitoring, Surveying and Evaluating the Environmental Health Program (No. 2021003330007)" funded by Korea Ministry of Environment, and the BioGreen 21 Program (No. PJ015708) funded by Rural Development Administration, Republic of Korea.

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