• 제목/요약/키워드: measuring activity of DO

검색결과 85건 처리시간 0.024초

DO 미터를 이용한 탐구중심 물 환경교육 프로그램 개발 - 용존산소 측정 활동 - (Development of Inquiry-based Water Environmental Education Program using DO Meter - Measuring Activity of Dissolved Oxygen -)

  • 류재홍;이두곤
    • 한국환경교육학회지:환경교육
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    • 제19권2호
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    • pp.96-107
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    • 2006
  • Inquiry has great value in environmental education(EE). Being able to see the world environmentally through 'inquiry-based environmental education' can be an important value and goal of EE. In this study, we intended to develop an EE program of measuring activity of dissolved oxygen(DO), based on the theory of 'inquiry-based EE'. Especially, we recognized the potential that DO meter can be used in 'inquiry-based EE', and we tried to develop a model of inquiry-based EE using DO meter. As a result of this research, we present specific models of inquiry-based EE about how to perform measuring activity of DO and how to use the DO meter in laboratories and streams from the perspective of inquiry of water environment. In the process of program development, we considered organization of the inquiry process, use of concept and knowledge, scientific inquiry and insightful inquiry, integration, sustain-ability, content components of 'Environmental Studies for EE', developmental level and in-forest of students. The developed EE model is a scientific inquiry model, pursuing 'explanation' based on data collection. Through this model, we tried to make students see water environment more deeply. The developed program can be applied to various water environments, like laboratories, streams, ponds, etc. It can be more effective inquiry activity if we perform measuring activities simultaneously with PH, electrical conductivity, and turbidity meters.

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강원도 자생 산채 추출물의 α-Amylase, α-Glucosidase, Lipase 효소 저해활성 탐색 (Screening of α-Amylase, α-Glucosidase and Lipase Inhibitory Activity with Gangwon-do Wild Plants Extracts)

  • 김희연;임상현;박유화;함헌주;이광재;박동식;김경희;김성문
    • 한국식품영양과학회지
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    • 제40권2호
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    • pp.308-315
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    • 2011
  • 연구는 강원도 자생 산채류의 다양한 약리 기능 중 ${\alpha}$-glucosidase, ${\alpha}$-amylase, lipase 효소 저해활성이 우수한 식물소재를 탐색하는데 있다. 강원도 양구, 평창, 태백에서 자생하고 있는 산채 90종을 채집하여 물과 에탄올로 각각 추출하였다. 항비만 활성 탐색은 ${\alpha}$-amylase, ${\alpha}$-glucosidase, lipase 저해활성을 검정하였다. 그 결과 산채 90종 중 ${\alpha}$-amylase 활성 저해능이 높은 식물로 에탄올 추출물 3종, 물추출물 2종, ${\alpha}$-glucosidase 활성 저해능이 높은 식물로 에탄올 추출물 13종, 물 추출물 3종, lipase 활성 저해능이 높은 식물로 에탄올 추출물 7종, 물 추출물 1종이 선발되었다. 향후 본 연구결과는 비만과 당뇨 등 대사성 질환에 대해 예방 혹은 치료 효과가 있는 기능성식품으로 개발하는데 있어 좋은 기초자료로 활용될 것으로 기대된다.

Free Radical Scavengers from the Fruits of Paeonia suffruticosa

  • Lee, Sa-Im;Yang, Jae-Heon;Baek, Nam-In;Kim, Sung-Hoon;Cho, Chong-Hyeon;Park, Byung-Hyun;Chae, Byeong-Suk;Kim, Dae-Keun
    • Natural Product Sciences
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    • 제14권3호
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    • pp.202-205
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    • 2008
  • The antioxidant activity of Paeonia suffruticosa (Paeoniaceae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract of P. suffruticosa showed the strong antioxidant activity. The antioxidant activity of ethyl acetate soluble fraction was stronger than that of the others ($IC_{50}$, $1.2\;{\mu}g/mL$), and the fraction was subjected to purification by repeated silica gel and Sephadex LH-20 column chromatography. Suffruticosol A, suffruticosol B, methyl gallate, and gallic acid, were isolated from the ethyl acetate soluble fraction. Their structures were elucidated by physico-chemical and spectroscopic studies.

Superoxide Radical Scavengers from the Whole Plant of Veronica peregrina

  • Ahn, Dal-Rae;Lee, Sa-Im;Yang, Jae-Heon;Cho, Chong-Hyeon;Hwang, Yong-Hun;Park, Jong-Han;Kim, Dae-Keun
    • Natural Product Sciences
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    • 제17권2호
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    • pp.142-146
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    • 2011
  • In the course of screening for antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl- 2-picrylhydrazyl (DPPH), a total extract from the whole plant of Veronica peregrina (Scrophulariaceae) was found to show potent antioxidant activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of six phenolic compounds including chrysoeriol (1), diosmetin (2), 4-hydroxybenzoic acid (3), apigenin (4), caffeic acid methylester (5) and protocatechuic acid (6). Their structures were elucidated by spectroscopic studies. Compounds 1-5 were isolated for the first time from this plant. Compounds 5 and 6 showed significant antioxidative effects in DPPH free radical scavenging and superoxide quenching activity assays.

호흡률 측정기를 이용한 제약폐수의 생물학적 활성 측정 (Measurement of Biological Activity in Pharmaceutic Wastewater by Using Respirometer)

  • 이영락;이기용;임지훈;이상훈;문흥만;심상준;이진원
    • KSBB Journal
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    • 제16권2호
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    • pp.183-187
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    • 2001
  • The biological activities of wastewater and sludge taken from the wastewater treatment process of Hyangnam pharmaceutic factories in Hwaseong, Kyeonggi-Do was measured using a respirometer. Oxygen uptake rate (OUR) was used as a tool for measuring biological activity. OUR was measured for varying amounts of sludge and organic chemicals in wastewater, and its toxicity was evaluated. Maximum OUR was observed as 61, 75, and 89 mg O$_2$/L/hr when sludge was added as 3, 5, and 10% of total volume, respectively. When the concentration of organic chemicals was changed to 1,486, 337, and 164 mg COD/L, maximum OUR was 53, 13, 8 mg O$_2$/L/hr, respectively. The toxicity test results showed that there seemed that there seemed to be no observable toxic effect on microbes in pharmaceutic wastewater.

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금강비 측정 교구 개발 및 체험수학활동

  • 김기원;도혜경
    • East Asian mathematical journal
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    • 제26권2호
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    • pp.281-299
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    • 2010
  • In this study we develop mathematical tools for measuring the Geumgang Ratio and we call them Geumgang Ratio Calipers. Also we show a teaching method for ratio through experimental mathematics. With Geumgang Ratio Calipers students measure the Geumgang Ratio in their bodies, materials for everyday life and the Sukgulam. Through this activity, students obtained an interest in mathematics and gained a positive attitude for mathematics.

The caloric expenditure of 1,000 Kcal per week can be a meaningful intervention for controlling coronary artery disease risk factors in older female adults

  • Joo, Kee-Chan
    • 보건교육건강증진학회지
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    • 제32권5호
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    • pp.73-81
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    • 2015
  • Objectives: We tried to confirm physical activity of 1,000 Kcal per week was a meaningful point in controlling coronary artery disease risks in female older adults. Methods: Participants were 66 female older adults recruited from senior welfare center. Participants were provided with accelerometer (e-step, Kenz, Japan) for measuring daily energy expenditure. Graded exercise test was done for measuring aerobic fitness. Blood glucose and lipid were analyzed. Framingham risk score was calculated based on blood glucose, blood lipid, and smoking. These variables were compared between the group expended more than 1,000 Kcal/week and the group with energy expenditure below 1,000 Kcal/week. Results: The group expended over 1,000kcal/week showed to be superior to the counterpart group in following variables; AC(Abdominal Circumference), %BF, $HR_{rest}$(resting heart rate), $VO_{2peak}$, FBG, LDL-C, TG, BDI-II, QOL, AR(Absolute Risk), RR(Relative Risk). Conclusions: The group expended over 1,000 Kcal/week was likely to have less probability in CAD than group expended less than 1,000 Kcal/week. The result of this study suggests the important role of active daily life that can be replaced with that of regular exercise especially for those who are not available to do structured exercise.

채취 시기 및 추출 용매에 따른 환삼덩굴의 항산화 및 기능성 화장품 활성 (Antioxidation and Functional Cosmetics Activity of Humulus japonicus Sieboid & Zucc. According to Collection Time and Extraction Solvent)

  • 채정우;조희선;염현지;이진영
    • 한국산림과학회지
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    • 제110권2호
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    • pp.254-265
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    • 2021
  • 채취 시기 및 추출 용매에 따른 환삼덩굴 추출물의 총 폴리페놀 함량을 측정한 결과 6월에 채취한 환삼덩굴의 열수 추출물의 총 폴리페놀 함량은 41.09 mg/g GAE, 같은 시기에 채취한 70% 에탄올 추출물은 56.64 mg/g GAE를 나타내었으며, 8월에 채취하여 추출한 열수 추출물은 31.56 mg/g GAE, 에탄올 추출물은 31.81 mg/g GAE의 함량을 나타내어, 열수와 에탄올 추출물 모두 6월에 채취하여 추출한 것이 총 폴리페놀 함량이 높게 나타났으며, 채취 시기가 같은 경우 열수 추출물보다 70% 에탄올 추출물의 총 폴리페놀 함량이 높은 것으로 나타났다. 전자공여능, SOD 유사활성, ABTS radical 소거능 측정을 통하여 환삼덩굴 추출물의 항산화 활성을 측정한 결과 모든 실험에서 총 폴리페놀 함량이 가장 높게 나타난 6월 채취 70% 에탄올 추출물의 활성이 가장 높았다. 또한 8월 채취 추출물의 경우 총 폴리페놀 함량은 비슷하였으나 모든 실험에서 에탄올 추출물의 활성이 높게 나타나 항산화에 관여하는 물질은 70% 에탄올로 추출하였을 때 활성이 높게 유지된다고 판단되었다. 미백활성 평가를 위한 tyrosinase 저해활성 측정 결과 환삼덩굴 열수 추출물은 6월 채취 추출물은 활성을 나타내지 않았으며 8월 채취 추출물은 최고농도에서의 활성이 16.18%로 나타났다. 에탄올 추출물의 경우 최고농도의 활성이 6월 채취 추출물은 8.07%, 8월 추출물이 14.7%로 나타나 미백활성을 나타내는 성분은 6월보다는 8월에 함량이 높은 것으로 판단되었다. 피부 주름 생성 방지 활성 평가를 위한 elastase 저해활성의 경우 에탄올 추출물은 매우 미미한 활성이 나타났으며 열수 추출물은 활성을 나타내지 않았다. 또한 모든 추출물에서 수렴활성이 나타나지 않아 환삼덩굴을 주름 생성 방지를 위한 기능성 원료로 사용하는 것은 적절하지 않다고 판단된다. 총 폴리페놀 함량과 항산화 활성이 가장 높게 나타난 6월 채취 환삼덩굴 에탄올 추출물의 세포독성(MTT assay) 결과, 저농도인 5~10 ㎍/mL에서는 세포증식효과를 나타내었으나 50 ㎍/mL에서 64.13%의 세포 생존율을 보여 강한 세포독성을 나타내었다. NO 생성 억제 능력의 경우, 농도가 증가함에 따라 Raw 264.7의 NO 생성이 억제되어 1,000 ㎍/mL에서의 NO 생성량이 40.7%까지 감소하였다. 그러나 이러한 결과가 세포 독성에 의한 것인지, 추출물의 효능에 의한 것인지는 추가 연구가 필요한 부분이다.

LSTM을 이용한 사용자 활동유형 및 인식기술 개발 (Development of user activity type and recognition technology using LSTM)

  • 김영균;김원종;이석원
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2018년도 추계학술대회
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    • pp.360-363
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    • 2018
  • 인간의 활동은 척추 옆굽음증, 골반 뒤틀림과 같은 개개인의 신체적 특징부터 기쁨, 분노, 슬픔 등의 감정들까지 다양한 요인들에 영향을 받는다. 하지만 이러한 동작의 특성은 오랜 시간에 걸쳐서 변화하며, 단기적으로 행동의 특성은 크게 변하지 않는다. 사람의 활동 데이터는 시간 흐름에 따라서 변화하는 시계열 적 특징과 각 행동별로 일정한 규칙성을 갖는다. 본 연구에서는 시계열 적 특징을 다루기 위한 순환신경망의 한 종류인 LSTM을 활동유형을 인식하는 기술에 적용하였으며, 측정시간과 LSTM 모델의 구성요소들에대한 파라미터 최적화로 활동유형의 인식률을 개선하였다.

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상황버섯(Phellinus linteus) 자실체 분비물의 면역활성 및 항암효과 (Immunomodulating and Antitumor Activities of Exo-secretion from Phellinus linteus)

  • 맹은호;이연태;조규봉;홍승희
    • IMMUNE NETWORK
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    • 제2권2호
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    • pp.115-124
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    • 2002
  • Background: The chemical characteristics of the exo-secretion from Phellinus linteus (referred to as exo-secretion) including the compositions of amino acids and monosaccharides were investigated. In addition, cytotoxicity of the exo-secretion on 5 tumor cell lines derived from human cancers and its antitumor activity against ascitic sarcoma-180 cells were examined. Methods: The antitumor activity of exo-secretion from Phellinus linteus was determined by measuring parameters including tumor weight, life span of mice, chemotatic activity of leukocytes, counts of immune cells, and activity of cytokines. Results: The exo-secretion from Phellinus linteus showed no direct cytotoxicity to the five tumor cell lines tested, but it had a strong antitumor activity against sarcoma-180 cells in ICR mice as measured by tumor weight and life span of mice. The exo-secretion stimulated the chemotaxis of leukocytes and production of immune cells and cytokines. Conclusion: These results suggest that the exo-secretion from Phellinus linteus do not act as a direct cytotoxic substance to cancer cells but as an immunomodulator.