Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Free Radical Scavengers from the Fruits of Paeonia suffruticosa

  • Lee, Sa-Im (College of Pharmacy, Woosuk University) ;
  • Yang, Jae-Heon (College of Pharmacy, Woosuk University) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, KyungHee University) ;
  • Kim, Sung-Hoon (College of Oriental Medicine, KyungHee University) ;
  • Cho, Chong-Hyeon (Jeollabuk-do Agricultural Research and Extension Servieces) ;
  • Park, Byung-Hyun (Department of Biochemistry, Medical School, Chonbuk National University) ;
  • Chae, Byeong-Suk (College of Pharmacy, Woosuk University) ;
  • Kim, Dae-Keun (College of Pharmacy, Woosuk University)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidant activity of Paeonia suffruticosa (Paeoniaceae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract of P. suffruticosa showed the strong antioxidant activity. The antioxidant activity of ethyl acetate soluble fraction was stronger than that of the others ($IC_{50}$, $1.2\;{\mu}g/mL$), and the fraction was subjected to purification by repeated silica gel and Sephadex LH-20 column chromatography. Suffruticosol A, suffruticosol B, methyl gallate, and gallic acid, were isolated from the ethyl acetate soluble fraction. Their structures were elucidated by physico-chemical and spectroscopic studies.

Keywords

References

  1. Chung, S.-C., Hwang, B. Y., Oh, G.-J., Kang, S. J., Kim, M.-J., Choi, W.- H., Lee, K. S., and Ro, J. S., Chemical components from the stem bark of Rhus javanica L. Kor. J. Pharmacogn. 30, 295-300 (1999)
  2. Kimura, T., But, P.P.H., Gue, J.-X., and Sung, C.K., International collation of traditional and folk medicine. Vol. 1. World scientific, Singapore, pp. 45-46 (1996)
  3. Li, G., Seo, C.S., Lee, K.S., Kim, H.J., Chang, H.W., Jung, J.S., Song, D.K., and Son, J.K., Protective constituents against sepsis in mice from the root cortex of Paeonia suffruticosa. Arch. Pharm. Res. 27, 1123-1126 (2004) https://doi.org/10.1007/BF02975116
  4. Lin, H.-C., Ding, H.-Y., and Wu, Y.-C., Two novel compounds from Paeonia suffructicosa. J. Nat. Prod. 61, 343-346 (1998) https://doi.org/10.1021/np9704258
  5. Lin, H.-C., Ding, H.-Y., Wu, T.-S., and Wu, P.-L., Monoterpene glycosides from Paeonia suffruticosa. Phytochemistry 41, 237-242 (1996) https://doi.org/10.1016/0031-9422(95)00526-9
  6. Matsuda, H., Ohta, T., Kawaguchi, A., and Yoshikawa, M., Bioactive constituents of chinese natural medicines. VI. Moutan cortex. (2): structures and radical scavenging effects of suffruticosides A, B, C, D, and E and galloyl-oxypaeoniflorin. Chem. Pharm. Bull. 49, 69-72 (2001) https://doi.org/10.1248/cpb.49.69
  7. Ryu, G., Park, E.K., Joo, J.H., Lee, B.H., Choi, B.W., Jung, D.S., and Lee, N.H., A new antioxidant monoterpene glycoside, alphabenzoyloxypaeoniflorin from Paeonia suffruticosa. Arch. Pharm. Res. 24, 105-108 (2001) https://doi.org/10.1007/BF02976476
  8. Sarker, S.D., Whiting, P., and Dinan, L., Identification and ecdysteroid antagonist activity of three resveratrol trimers (Suffruticososols A, B and C) from Paeonia suffruticosa. Tetrahedron 55, 513-524 (1999) https://doi.org/10.1016/S0040-4020(98)01049-7
  9. Takechi, M. and Tanaka, Y., Antiviral substances from the root of paeonia species. Planta Med. 45, 252-253 (1982) https://doi.org/10.1055/s-2007-971387
  10. Wang, S.J., Yang, Y.C., Li, S., and Shi, J.G., A new paeoniflorin derivative isolated from the root bark ethanol extract of Paeonia suffruticosa. Zhongguo Zhong Yao Za Zhi. 30, 759-761 (2005a)
  11. Wang, X., Cheng, C., Sun, Q., Li, F., Liu, J., and Zheng, C., Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography. J. Chromatogr. A. 1075, 127-131 (2005b) https://doi.org/10.1016/j.chroma.2005.04.017
  12. Yoshica, T., Mori, K., Hatano, T., Okumura, T., Uehara, L., Komagoe, K., Fujita, Y., and Okuda, T., Studies on inhibition mechanism of autooxidation by tannins and flavonoids. V. Radical scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 1919-1921 (1989) https://doi.org/10.1248/cpb.37.1919
  13. Yoshikawa, M., Ohta, T., Kawaguchi, A., and Matsuda, H., Bioactive constituents of Chinese natural medicines. V. Radical scavenging effect of Moutan Cortex. (1): Absolute stereostructures of two monoterpenes, paeonisuffrone and paeonisuffral. Chem. Pharm. Bull. 48, 1327-1331 (2000) https://doi.org/10.1248/cpb.48.1327
  14. Yoshikawa, M., Uchida, E., Kawaguchi, A., Kitagawa, I., and Yamahara, J., Galloyl-oxypaeoniflorin, suffruticosides A, B, C, and D, five new antioxidative glycosides, and suffruticoside E, A paeonol glycoside, from Chinese moutan cortex. Chem. Pharm. Bull. 40, 2248-2250 (1992) https://doi.org/10.1248/cpb.40.2248