• 한국식품영양학회지
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• 제24권3호
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• pp.291-294
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• 2011

A lipase inhibitor from Desmodium oxyphyllum DC. was purified by methanol extraction, systematic solvent extraction, silica gel column chromatography, $C_{18}$ solid phase extraction chromatography and RP-HPLC. We obtained the purified lipase inhibitor with 182 ng($IC_{50}$) of lipase inhibitory activity for a 0.06% yield. Its molecular weight was estimated to be 655.37 Da from an instrumental analysis of MALDI-TOF-MS and it was identified copper-3,5-dibromo-2-hydroxybenzoic acid ($C_{14}H_8Br_4CuO_6$) by $^1H$, $^{13}C$ NMR analysis.

• 한국식품과학회지
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• 제35권3호
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• pp.519-526
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• 2003

본 연구에서는 한국인에게 적합한 비만 개선책을 찾고자 지방 흡수억제 기천뿐만 아니라 과잉의 탄수화물 흡수억제가 함께 작용하는 분화억제에 대한 지방흡수 억제 물질과 율무 열수에서 ${\alpha}$-amylase 활성 저해에 대한 탄수화물 흡수 억제물질이 첨가된 비만 개선 식이조성물이 동물 실험 시 체중 증가가 억제됨을 확인하였다. 호박의 열수 추출물을 ethyl acetate로 추출하여 췌장 lipase 활성 저해를 확인하였으며, 호박의 ethyl acetate 추출물을 농축하여 silica gel column chromatography와 C-18 column chromatography를 이용하여 활성 저해 물질을 분리하였다. 또한 율무 열수는 chloroform으로 추출하여 호박 열수와 같은 방법으로 분리하여 ${\alpha}$-amylase 활성 저해를 확인하였다. 3T3-L1 분화억제 확인 실험에서는 3T3-L1에 분화 유도물질만 처리한 경우 지방 세포로 분화되어 세포내에 지방축적이 일어났지만, 분화 유도물질과 호박 추출물을 동시처리 결과 지방 세포로의 분화가 호박 추출물 $120\;{\mu}g/mL$ 처리 시약 5% 이하로 일어났으며, 세포내에 지방축적도 거의 일어나지 않았다. 동물실험에서 고지방 식이와 비만개선용 식이조성물을 동시에 섭취한 군의 체중 증가가 고지방 식이만 섭취한 군보다 13% 정도 감소함을 확인할 수 있었고, 비만개선용 식이 조성물을 8주간 경구 투여한 뒤 간 기능 지표와 혈액학적 지표 및 흰쥐의 행동학적 소견에서 차이를 나타내지 않아 비만개선용 식이조성물이 경구 투여에 의한 안전성이 있는 것으로 확인되었다. 이들 결과를 종합하면, 호박의 췌장 lipase 활성 저해물질과 율무의 ${\alpha}$-amylase 활성 저해물질을 첨가한 비만개선용 식이조성물이 동물 실험에서 체중 증가를 효과적으로 억제할 수 있음을 관찰하였으며, 이는 비만 환자들의 다이어트 식이로 유용할 것으로 사료되었다.

• 한국식품저장유통학회지
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• 제24권3호
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• pp.483-489
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• 2017

본 연구에서는 저온 플라즈마를 활용하여 phloridzin을 20, 40 및 60분 동안 처리하여 얻어진 결과물에 대하여 DPPH 라디칼 소거, ${\alpha}$-glucosidase 및 pancreatic lipase 저해활성을 평가하였다. 그 결과, DPPH 라디칼 소거활성은 플라즈마로 처리하지 않은 phloridzin의 $IC_{50}$ 값이 $500{\mu}g/mL$ 이상이었으며, 이와 비교하여 플라즈마 60분 처리 반응물의 경우 $IC_{50}$ 값이 $100.5{\pm}2.9{\mu}g/mL$ 나타내어 항산화 활성이 상승한 것을 확인하였다. 또한 ${\alpha}$-glucosidase 저해활성은 60 분 동안 플라즈마 처리한 phloridzin의 $IC_{50}$ 값이 $43.5{\pm}2.2{\mu}g/mL$의 가장 우수한 저해활성을 나타내었으며 이 활성은 양성대조군인 acarbose의 $IC_{50}$ 값인 $541.3{\pm}1.9{\mu}g/mL$ 보다 10배 이상 강한 활성이었다. Pancreatic lipase 저해활성은 60분 플라즈마를 처리한 phloridzin의 $IC_{50}$ 값이 $29.8{\pm}2.0{\mu}g/mL$로 가장 우수한 활성을 나타내었다. 향후 이들 반응을 통하여 생성된 화합물의 대량생산을 통한 물질 분리 및 구조 동정을 통하여 DPPH 라디칼 소거활성, ${\alpha}$-glucosidase 및 pancreatic lipase 저해활성 메커니즘 검증을 수행할 필요성이 있다고 사료된다.

• Preventive Nutrition and Food Science
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• 제15권4호
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• pp.249-254
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• 2010

We examined the effect of extracts of meju prepared with traditional and standardized methods on pancreatic lipase and the absorption of dietary lipid. Aqueous methanolic (80%, v/v) extracts of meju dose-dependently inhibited the activities of porcine pancreatic lipase. The plasma triglyceride levels in Imprinting Control Region mice after a single oral administration of lipid emulsion containing aqueous methanolic extracts from meju made by the standardized methods were lower than that of the group given a lipid emulsion containing the extracts of meju made by traditional methods. The inhibitory activity of the meju extract on dietary lipid digestion appears to be more closely associated with aglycone forms of phenolic compounds such as free isoflavones than with glycosides, since meju samples with higher total phenolic or free isoflavone content showed the stronger inhibition against pancreatic lipase. Furthermore, the data suggest that meju made using the standardized method, which contains higher levels of total isoflavones relative to traditionally prepared meju, could effectively suppress digestion of dietary lipids and therefore have the potential to help ameliorate hyperlipidemia and obesity.

• 한국식품저장유통학회지
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• 제25권1호
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• pp.124-135
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• 2018

본 연구는 영지버섯(Ganoderma lucidum)의 영양학적 특성과 생리활성을 조사하였다. 탄수화물 함량은 원형 영지버섯이 높았고 조회분, 조지방, 조단백질 및 무기질 함량은 녹각 영지버섯이 높았다. 주요 아미노산, 지방산, 무기질 및 수용성 비타민은 valine(원형: 11.90 mg/g 및 녹각: 17.18 mg/g), linoleic acid(원형: 47.56% 및 녹각: 75.68%), 칼륨(원형: 116.50 mg/100 g 및 녹각: 184.36 mg/100 g) 및 비타민 $B_3$(원형: 1.78 mg/100 g 및 녹각: 1.81 mg/100 g) 있었다. 한편 ${\beta}$-glucan 함량은 각각 34.15 g/100 g(원형)과 30.07 g/100 g(녹각)로 검출되었다. 추출조건 중 $40^{\circ}C-70%$ 에탄올 추출물에서 가장 우수한 라디칼 소거활성과 탄수화물 및 지방분해 효소 저해활성을 나타내었다. 특히, $40^{\circ}C-70%$ 에탄올로 추출한 원형영지버섯의 1 mg/mL 처리 시 DPPH, ABTS 및 hydroxyl 라디칼 소거활성과 ${\alpha}$-glucosidase, ${\alpha}$-amylase 및 pancreatic lipase 저해활성은 각각 92.85, 99.74, 58.09, 89.68, 44.68, 67.56%로 나타났다.

• 한국식품영양과학회지
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• 제28권4호
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• pp.816-821
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• 1999

Cholesterol esterase(pCEH, pancreas cholesterol ester hydrolase, E.C.3.1.1.13) which is secreted from pancreas has been known as an important lipase for cholesterol uptake. cholesteryl acyl esters from a diet must be hydrolyzed to free cholesterol and fatty acid by cholesterol esterase before the absorption in small intestine. For the development of inhibitory substances from natural source, we screened many extracts of oriental herbs for the inhibition of cholesterol esterase in vitro. The ethanol extract of Ephedra herba showed strong inhibitory activity. Solvent fractionation and silica gel column chromatography with the extract lead to the purification of the inhibitory principle in Ephedra herba. Crystallized inhibitor was identified as ( ) ephedrine by using UV, FT IR, 1H NMR, 13C NMR and GC/Mass. These results suggest that ( ) ephedrine can be used as a potential lead compound for the development of inhibitor for cholesterol uptake by cholesterol esterase inhibition.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.458-463
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• 2022

We evaluated the antibiotic susceptibilities, hemolytic activities, and technological properties of 36 Staphylococcus xylosus strains and 49 S. pseudoxylosus strains predominantly isolated from fermented soybean foods from Korea. Most of the strains were sensitive to chloramphenicol, erythromycin, gentamycin, kanamycin, lincomycin, oxacillin, tetracycline, and trimethoprim. However, 23 strains exhibited potential phenotypic acquired resistance to erythromycin, lincomycin, and tetracycline. Based on breakpoint values for staphylococci from the Clinical and Laboratory Standards Institute, >30% of the isolates were resistant to ampicillin and penicillin G, but the population distributions in minimum inhibitory concentration tests were clearly different from those expected for acquired resistance. None of the strains exhibited clear α- or β-hemolytic activity. S. xylosus and S. pseudoxylosus exhibited salt tolerance on agar medium containing 20% and 22% (w/v) NaCl, respectively. S. xylosus and S. pseudoxylosus strains possessed protease and lipase activities, which were affected by the NaCl concentration. Protease activity of S. pseudoxylosus was strain-specific, but lipase activity might be a characteristic of both species. This study confirms the potential of both species for use in high-salt soybean fermentation, but the safety and technological properties of strains must be determined to select suitable starter candidates.

• 한국식생활문화학회지
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• 제36권1호
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• pp.130-142
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• 2021

This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.

• 한방비만학회지
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• 제21권2호
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• pp.69-79
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• 2021

Objectives: This study aimed to compare the anti-obesity effects of raw and pickled garlic in vitro in 3T3-L1 preadipocytes. Methods: The pickled garlic samples comprised the following: garlic aged in vinegar (VG), garlic aged in soy sauce, and vinegar (1:1, v/v) (PG) and raw garlic (RG) as control. Hexane, butanol, and distilled water were used to prepare the fractions. The pancreatic lipase inhibitory activity was used as a measure of anti-obesity effects of the extracts. The lipid droplet accumulation and triglyceride content in the 3T3-L1 cells were measured using Oil red O staining and triglyceride assay kits, respectively. The adipogenesis related protein expression levels were analyzed using the kits and the western blot method. Results: The pancreatic lipase inhibitory activity of the garlic extracts (VG, PG, RG) was the highest in the butanol fraction, and the inhibitory effect was the highest in RG, followed by PG and VG. All garlic butanol extracts suppressed triglyceride accumulation in differentiated adipocytes (P<0.05) through the activation of cyclic adenosine monophosphate (AMP), AMP-activated protein kinase, carnitine palmitoyl transferase-1, and the inhibition of fatty acid synthase. Raw garlic extracts significantly inhibited the expression of proteins involved in adipogenesis as compared to pickled garlic. Conclusions: Raw garlic has the potential to be an effective natural material for reducing obesity compared to pickled garlic with vinegar or soy sauce.

• 한방재활의학과학회지
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• 제30권4호
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• pp.41-53
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• 2020

Objectives The purpose of this study was to investigate the effects of Silbi-san on the antioxidant and fat accumulation inhibition and to analyze the anti-obesity effect by analyzing the changes in serum lipid composition in obese mice. Methods We compared contents of phytochemicals like total polyphenols and total flavonoid and antioxidant activities such as 2,2-dipheny-1-picrylhydrazyl and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity. After Silbi-san in 3T3-L1 cells in vitro and mouse adipose tissue ex vivo, we quantified intracellular triglyceride accumulation and lipolysis. Moreover, the anti-obesity activity though inhibiting pancreatic lipase were analyzed. In 3T3-L1 cells, morphological changes showed that control cells had many lipid while cells treated with Silbi-san had less lipid accumulation. 30% EtOH Silbi-san treatment also suppressed the fat absorption by inhibiting the activity of pancreatic lipase and led to high lipolysis through promoting glycerol release. The experimental group was divided into four groups: Normal group fed normal feed, Control group fed 60% high fat diet (HFD) and distilled water, drug group fed 60% high fat diet and 200 mg/kg of Silbi-san water extract, drug group fed 60% HFD and 200 mg/kg of Silbi-san 30% ethanol extract. Results Serum total cholesterol content and serum low density lipoprotein-cholesterol content were significantly decreased in the Silbi-san extract group compared to the control group, serum high density lipoprotein-cholesterol content was significantly increased in Silbi-san extract group. Conclusions In this study, the antioxidant and fat accumulation inhibitory effects of Silbi-san were confirmed.